Bioethanol production from sugarcane bagasse by simultaneous sacarification and fermentation using Saccharomyces cerevisiae

Hernawan,; Maryana, Roni; Pratiwi, Diah; Wahono, Satriyo Krido; Darsih, Cici; Hayati, Sri; Poeloengasih, Crescentiana Dewi; Nisa, Khoirun; Indrianingsih, Anastasia Wheni; Prasetyo, Dwi Joko; Jatmiko, Tri Hadi; Kismurtono, M.; Rosyida, Vita Taufika

doi:10.1063/1.4978099

Cited by 17 publications

(7 citation statements)

References 16 publications

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“…Phanerochaete chrysosporium can produce crude cellulase with greater activity than using other microbes. In a study on bioethanol production using bagasse with crude cellulase from Phanerochaete chrysosporium, using dry yeast, the best results were obtained with fermented ethanol with a concentration of 9.22% with a fermentation time of 96 h. The results of this study showed an increase in the concentration of ethanol when compared to previous studies, for example in the research of Bhatia and Johri, 2018, producing ethanol with a concentration of 9.15 g/L at 72 h incubation, with Pachysolen tannophilus MTCC 1077, and producing ethanol with a concentration of 6.83 ± 0.07% with commercial enzymes [9].…”

Section: Introductionsupporting

confidence: 50%

“…The highest ethanol concentration achieved in this study was 9.18%, where the bioethanol content produced was higher than previous studies because each researcher uses different types of enzymes for the hydrolysis process, also the addition of Saccharomyces cerevisiae is different, both in the phase and concentration of addition, also because other operating conditions are different [9,15,22].…”

Section: Effect Of the Amount Of Crude Cellulase And Added Saccharomy...mentioning

confidence: 71%

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Utilization of Bagasse for Bioethanol Raw Materials Using Crude Cellulase from Phanerochaete Chrysosporium with SSF Method

Rulianah¹,

Prayitno²,

Maulidina³

2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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Bagasse is one of the solid wastes from sugar factories which contains lignocellulose. Lignocellulose contains high levels of cellulose so that it has the potential as raw material to produce bioethanol. The crude cellulase produced by Phanerochaete chrysosporium has enzymes capable of degrading lignin, cellulose and hemicellulose simultaneously. The aim of the research is to analyses the effect of adding crude cellulose and Saccharomyces cerevisiae to the yield and concentration of bioethanol. The research was conducted by drying and reducing the size of the bagasse, cultivating of the Phanerocheate chrysoporium, fermenting the bagasse, and filtering. The resulting crude cellulase activity will be tested using the DNS method. The best crude cellulase activity is used to make bioethanol from bagasse as raw material. Simultaneous Saccharification and Fermentation (SSF) method is used in the production of bioethanol. The concentration of the bioethanol product was analysed using Gas Chromatography. The variables used are the addition of 6% bagasse, crude cellulose 40, 50, and 60% (v/v), Saccharomyces cerevisiae 1, 2, 3, 4, and 5% (w/v). In this study, the best conditions were obtained by adding 60% crude cellulase and 4% Saccharomyces cerevisiae, obtained bioethanol with a concentration of 9.18%, and a yield of 16.79%.

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Section: Introductionsupporting

confidence: 50%

Section: Effect Of the Amount Of Crude Cellulase And Added Saccharomy...mentioning

confidence: 71%

Utilization of Bagasse for Bioethanol Raw Materials Using Crude Cellulase from Phanerochaete Chrysosporium with SSF Method

Rulianah¹,

Prayitno²,

Maulidina³

2022

Proceedings of the 4th International Conference on Life Sciences and Biotechnology (ICOLIB 2021)

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“…Chemical composition of yam peel (lignin, hemicelluloses and cellulose) was determined before and after pretreatment according to the method of Hernawan et al, (2017). 1g (a) of sample was added to 150ml of water and boiled for 1hour at 100 0 C. It was washed with 300ml of hot water, dried and weighed (b).…”

Section: Compositional Analysis Of Biomassmentioning

confidence: 99%

Pretreatment of Millet Husk Using Alkaline Hydrogen Peroxide to Enhance Enzymatic Hydrolysis for Reducing Sugar Production

Saulawa¹,

Nura²,

Bala³

et al. 2021

FJS

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The effectiveness of alkaline hydrogen peroxide as a suitable choice of pretreatment for the conversion of millet husk to reducing sugars using cellulase enzyme for hydrolysis and subsequent ethanol production was determined. The effects of three variables on reducing sugar production from millet husk were determined using one factor at a time (OFAT) method namely; peroxide concentration, pretreatment time and pretreatment temperature. From the results, it was observed that a significant (P<0.05) amount of reducing sugars were lost during pretreatment of millet husk. The untreated group which was only physically pretreated (milled) however yielded a significantly higher (P<0.05) reducing sugar concentration of 10.67mg/ml after enzymatic hydrolysis while the highest reducing sugar concentration of 4.82mg/ml was obtained using 0.375%v/v peroxide concentration for 60minutes at 250C. Therefore, pretreatment of biomass with alkaline hydrogen peroxide may be more suitable for feedstock with high lignin contents than millet husk.

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“…The lignin, cellulose and hemicelluloses content of both rice bran were analyzed using Chesson method as described by Hernawan et al [14]. To one g (a) of dry sample, 150 ml water was added and refluxed at 100 °C in water bath for 1 hour.…”

Section: Complex Carbohydrate Composition Of Rice Branmentioning

confidence: 99%

Potential of Bran from Two Varieties of Rice (Oryza) Spp for Bioethanol Production

Musa

Abdullahi

El-Yakub

et al. 2020

JABB

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Second generation ethanol is produced from non-food based including waste from food crops, wood chips and agricultural residue. Lignocellulosic and starchy materials in them are converted to fermentable sugars which are further processed to produce bioethanol. Rice bran is an agricultural residue with abundant carbohydrate for bioconversion into ethanol. This study was designed to evaluate the potential of two varieties of rice bran (Sipi and Wita) to produce bioethanol. Compositional analysis of Wita rice bran showed 40% cellulose, 23% hemicellulose and 16% lignin content. Sipi variety contains 35% cellulose, 27% hemicellulose and 13% lignin content. Sodium hydroxide pretreatment was carried out at different concentrations (0.5%, 1%, 2% and 3%) and residence time of (15, 30, 60, and 90min). It was observed from the present study, pretreatment of rice bran with 2% NaOH for 90min is considered as effective pretreatment condition for bioethanol production from rice bran. Simultaneous saccharification and fermentation of cellulosic biomass was carried out for 72h with Saccharomyces cerevisae and Mucor indicus. Fermentation of Wita variety with S.cerevisiae produced highest bioethanol yield of 1.36% while Mucor indicus produced 0.75% bioethanol yield. From the result of these findings, it can be concluded that rice bran could be considered as a promising substrate for the fermentation of second generation ethanol.

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Bioethanol production from sugarcane bagasse by simultaneous sacarification and fermentation using Saccharomyces cerevisiae

Cited by 17 publications

References 16 publications

Utilization of Bagasse for Bioethanol Raw Materials Using Crude Cellulase from Phanerochaete Chrysosporium with SSF Method

Utilization of Bagasse for Bioethanol Raw Materials Using Crude Cellulase from Phanerochaete Chrysosporium with SSF Method

Pretreatment of Millet Husk Using Alkaline Hydrogen Peroxide to Enhance Enzymatic Hydrolysis for Reducing Sugar Production

Potential of Bran from Two Varieties of Rice (Oryza) Spp for Bioethanol Production

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