Utilization of Bagasse for Bioethanol Raw Materials Using Crude Cellulase from Phanerochaete Chrysosporium with SSF Method

Abstract

Bagasse is one of the solid wastes from sugar factories which contains lignocellulose. Lignocellulose contains high levels of cellulose so that it has the potential as raw material to produce bioethanol. The crude cellulase produced by Phanerochaete chrysosporium has enzymes capable of degrading lignin, cellulose and hemicellulose simultaneously. The aim of the research is to analyses the effect of adding crude cellulose and Saccharomyces cerevisiae to the yield and concentration of bioethanol. The research was conducted by drying and reducing the size of the bagasse, cultivating of the Phanerocheate chrysoporium, fermenting the bagasse, and filtering. The resulting crude cellulase activity will be tested using the DNS method. The best crude cellulase activity is used to make bioethanol from bagasse as raw material. Simultaneous Saccharification and Fermentation (SSF) method is used in the production of bioethanol. The concentration of the bioethanol product was analysed using Gas Chromatography. The variables used are the addition of 6% bagasse, crude cellulose 40, 50, and 60% (v/v), Saccharomyces cerevisiae 1, 2, 3, 4, and 5% (w/v). In this study, the best conditions were obtained by adding 60% crude cellulase and 4% Saccharomyces cerevisiae, obtained bioethanol with a concentration of 9.18%, and a yield of 16.79%.