Bioethanol Production from Cellulose-Rich Corncob Residue by the Thermotolerant Saccharomyces cerevisiae TC-5

Boonchuay, Pinpanit; Techapun, Charin; Leksawasdi, Noppol; Seesuriyachan, Phisit; Hanmoungjai, Prasert; Watanabe, Masanori; Srisupa, Siraprapa; Chaiyaso, Thanongsak

doi:10.3390/jof7070547

Cited by 26 publications

(15 citation statements)

References 58 publications

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“…The l -lactate and d -lactate concentrations in the supernatants were analysed using an l , d -lactate Assay Kit (Nanjing Jiancheng Institute of Biological Engineering, Nanjing, China). The ethanol concentration was determined using gas chromatography according to the modified method of Boonchuay et al ( 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Roughage biodegradation by natural co-cultures of rumen fungi and methanogens from Qinghai yaks

et al. 2022

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Anaerobic fungus–methanogen co-cultures from rumen liquids and faeces can degrade lignocellulose efficiently. In this study, 31 fungus–methanogen co-cultures were first obtained from the rumen of yaks grazing in Qinghai Province, China, using the Hungate roll-tube technique. The fungi were identified according to morphological characteristics and internal transcribed spacer (ITS) sequences. The methanogens associated with each fungus were identified by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) and 16S rRNA gene sequencing. They were five co-culture types: Neocallimastix frontalis + Methanobrevibacter ruminantium, Neocallimastix frontalis + Methanobrevibacter gottschalkii, Orpinomyces joyonii + Methanobrevibacter ruminantium, Caecomyces communis + Methanobrevibacter ruminantium, and Caecomyces communis + Methanobrevibacter millerae. Among the 31 co-cultures, during the 5-day incubation, the N. frontalis + M. gottschalkii co-culture YakQH5 degraded 59.0%–68.1% of the dry matter (DM) and 49.5%–59.7% of the neutral detergent fiber (NDF) of wheat straw, corn stalk, rice straw, oat straw and sorghum straw to produce CH4 (3.0–4.6 mmol/g DM) and acetate (7.3–8.6 mmol/g DM) as end-products. Ferulic acid (FA) released at 4.8 mg/g DM on corn stalk and p-coumaric acid (PCA) released at 11.7 mg/g DM on sorghum straw showed the highest values, with the following peak values of enzyme activities: xylanase at 12,910 mU/mL on wheat straw, ferulic acid esterase (FAE) at 10.5 mU/mL on corn stalk, and p-coumaric acid esterase (CAE) at 20.5 mU/mL on sorghum straw. The N. frontalis + M. gottschalkii co-culture YakQH5 from Qinghai yaks represents a new efficient combination for lignocellulose biodegradation, performing better than previously reported fungus–methanogen co-cultures from the digestive tract of ruminants.

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Section: Methodsmentioning

confidence: 99%

Roughage biodegradation by natural co-cultures of rumen fungi and methanogens from Qinghai yaks

et al. 2022

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“…The replication plasmid YRp of Saccharomyces cerevisiae is unstable (Xie et al, 2018) It can be used as an expression system for the inhibition of human dipeptidyl peptidase IV/ CD26 (hDPPIV) (Zhang et al, 2016). It can produce hormones (Chigira et al, 2008), functional foods (Lahue et al, 2020), and biofuels (Boonchuay et al, 2021;Hong and Nielsen, 2012), and itself can also be used as probiotics…”

Section: Saccharomyces Cerevisiaementioning

confidence: 99%

1Progress, applications, challenges and prospects of protein purification technology

Hou

Liu

et al. 2022

Front. Bioeng. Biotechnol.

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Protein is one of the most important biological macromolecules in life, which plays a vital role in cell growth, development, movement, heredity, reproduction and other life activities. High quality isolation and purification is an essential step in the study of the structure and function of target proteins. Therefore, the development of protein purification technologies has great theoretical and practical significance in exploring the laws of life activities and guiding production practice. Up to now, there is no forthcoming method to extract any proteins from a complex system, and the field of protein purification still faces significant opportunities and challenges. Conventional protein purification generally includes three steps: pretreatment, rough fractionation, and fine fractionation. Each of the steps will significantly affect the purity, yield and the activity of target proteins. The present review focuses on the principle and process of protein purification, recent advances, and the applications of these technologies in the life and health industry as well as their far-reaching impact, so as to promote the research of protein structure and function, drug development and precision medicine, and bring new insights to researchers in related fields.

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“…Additionally, quite a few of the S. cerevisiae strains characterized by resistance to high temperature were isolated and reported; thermotolerant IR2-9a (Edgardo et al, 2008), PTCC 5269 M 3 and Areni M 7 (Mehdikhani et al, 2011), mbc 2 (Cha et al, 2015), DBKKU Y-53 (Nuanpeng et al, 2016), KKU-VN8 (Techaparin et al, 2017), DMKU 3-S087 (Pattanakittivorakul et al, 2019), and TC-5 (Boonchuay et al, 2021) exhibited remarkable advantages for ethanol production, particularly from sweet sorghum, molasses, and corncob residue, at temperatures that are all equal to or greater than 40 °C; further research efforts with OMICs sequencing and analysis of the above mutants are required for strain improvement toward application.…”

Section: Thermal Tolerancementioning

confidence: 99%

OMICs-Based Strategies to Explore Stress Tolerance Mechanisms of Saccharomyces cerevisiae for Efficient Fuel Ethanol Production

Mehmood

Wang

et al. 2022

Front. Energy Res.

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Efficient biotransformation of lignocellulosic biomass to second-generation (2G) bioethanol requires promising strains harboring built-in resistance against limitations imposed by pretreated lignocellulose-derived compounds. Ethanol fermentation and stress tolerance of yeast cells are almost simultaneously exposed to sequence variations and multiple inhibitory factors during the phases of proliferation, metabolism, and productivity. Several studies have extensively concentrated on identification or characterization of genes which confer resistance to various stresses and yeast tolerance enhancement through genetic breeding. However, the investigation of individual genes is inadequate to explain the global molecular mechanism. Herewith, “OMICs-approaches,” including genomics, transcriptomics, proteomics, and metabolomics, which are comprehensively aimed at comparative, functional profiling of the whole metabolic network, have elucidated complex cellular reactions under stressful conditions. This review briefly discusses the research progress in the field of multi-OMICs with a special focus on stress-responsive factors in frequently used S. cerevisiae. It also highlights how to promote metabolic-engineered strains for increased tolerance and higher production yield, which should be deeply exploited to achieve robustness during the lignocellulose-to-ethanol conversion process.

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Bioethanol Production from Cellulose-Rich Corncob Residue by the Thermotolerant Saccharomyces cerevisiae TC-5

Cited by 26 publications

References 58 publications

Roughage biodegradation by natural co-cultures of rumen fungi and methanogens from Qinghai yaks

Roughage biodegradation by natural co-cultures of rumen fungi and methanogens from Qinghai yaks

1Progress, applications, challenges and prospects of protein purification technology

OMICs-Based Strategies to Explore Stress Tolerance Mechanisms of Saccharomyces cerevisiae for Efficient Fuel Ethanol Production

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