Fresh juice from C grade longan (mg/g fresh fruit; total sugars 51.6 ± 0.5, nitrogen 0.021 ± 0.004; gallic acid 0.025 ± 0.001; ellagic acid 0.016 ± 0.001) with addition of 8.52 g ammonium sulfate/l was the optimal medium for cultivation processes of Saccharomyces cerevisiae TISTR 5606 and Candida tropicalis TISTR 5306 capable of producing ethanol and phenylacetylcarbinol (PAC) among five longan grades. S. cerevisiae TISTR 5606 produced ethanol (33.4 ± 3.2 g/L) at a significantly higher level (p ≤ 0.05) than C. tropicalis TISTR 5306 (22.3 ± 1.1 g/L) with similar ethanol yields (Yp/s) between 0.21 and 0.22 g ethanol produced/g sugars consumed. Whole cells of C. tropicalis TISTR 5306 produced a significantly higher (p ≤ 0.05) PAC level (27.2 ± 0.7 mM) than S. cerevisiae TISTR 5606 (3.59 ± 1.33 mM) after 6 hr in an equivalent volume biphasic biotransformation system.
Practical applications
Longan is one of the important economic fruit of Thailand with production volume in 2017 reaching one million tons. Less than 10% of these are domestically consumed while more than 90% are exported to several countries whose population frequently consume longan as a nutritious food supplement. This study can help solving overproduction problem of longan fruit by processing C grade fresh longan, which is accounted for 5% of the overall production volume, in the form of juice extract with the relatively high sugars content. Microbial fermentation of this juice and subsequent whole cells biotransformation process result in ethanol and PAC, respectively. Ethanol can be used as an alternative biofuel or important industrial solvent while PAC is a precursor for production of commercial nasal decongestant (ephedrine) or anti‐asthmatic compound (pseudoephedrine).
Cellulose from Asparagus officinalis stalk end was extracted and synthesized to carboxymethyl cellulose (CMCas) using monochloroacetic acid (MCA) via carboxymethylation reaction with various sodium hydroxide (NaOH) concentrations starting from 20% to 60%. The cellulose and CMCas were characterized by the physical properties, Fourier Transform Infrared spectroscopy (FTIR), Differential scanning calorimetry (DSC), Scanning electron microscopy (SEM) and X-ray diffraction (XRD). In addition, mechanical properties of CMCas films were also investigated. The optimum condition for producing CMCas was found to be 30% of NaOH concentration for the carboxymethylation reaction, which provided the highest percent yield of CMCas at 44.04% with the highest degree of substitution (DS) at 0.98. The melting point of CMCas decreased with increasing NaOH concentrations. Crystallinity of CMCas was significantly deformed (p < 0.05) after synthesis at a high concentration. The L* value of the CMCas was significantly lower at a high NaOH concentration compared to the cellulose. The highest tensile strength (44.59 MPa) was found in CMCas film synthesized with 40% of NaOH concentration and the highest percent elongation at break (24.99%) was obtained in CMCas film treated with 30% of NaOH concentration. The applications of asparagus stalk end are as biomaterials in drug delivery system, tissue engineering, coating, and food packaging.
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