Biodegradation of dairy wastes using crude enzymatic extract of Yarrowia lipolytica ATCC 9773

Abstract

Effluents generated by the food industry have become a serious environmental concern. Bioremediation is a biological process developed as an alternative for the treatment of contaminated areas. In current research, the biodegradation of fat, Biochemical Oxygen Demand (BOD5), Chemical Oxygen Demand (COD) and total solids were evaluated in dairy waste employing enzymatic extract of Yarrowia lipolytica ATCC 9773 as biological agents. All the variables were determined following the specifications of the Standard Methods of the American Water Works Association. Enzymatic extract of Y. lipolytica at different concentrations (8, 12 and 16.0%) was used in a fermentative medium at two pHs (5.0 and 6.5) for 32 h. The highest percentages (%) of fat (82.88), BOD (43.32), COD (44.3) and total solids (13.58) removal were obtained using an inoculum concentration of 16% at pH 5.0 for 32 h of fermentation. These results may have industrial relevance for the reduction of contamination of industrial effluents with high levels of fat and other contaminants.

Use of Non-Conventional Yeast Yarrowia lipolytica in Treatment or Upgradation of Hydrophobic Industry Wastes

“…According to the removal percentage COD and BOD as parameters that prove the effectiveness of treatment. The best results correspond to: inoculum concentration 16%, pH: 5 (BOD removal-43.07%) and inoculum concentration 8%, pH: 6.5 (BOD removal-37.93%) [55].…”

“…Yarrowia lipolytica can not only use the waste to grow and produce valuable metabolites, but also contribute to reducing the BOD (biochemical oxygen demand) of such a waste. Dunoyer et al [55] made some intriguing observations, when fatty effluent from dairy industry with high BOD, COD and high fat and dry matter content was treated by crude enzymatic extract (CEE) from Y. lipolytica ATCC 9773 cultivation. The strain decreased the lipid content in the waste by 82.88% and reduced the levels of BOD5 until reaching the values of 43.32% respectively.…”

Production of sustainable biodiesel from alkaline pretreated dairy effluent using Yarrowia lipolytica

“…Due to Y . lipolytica 's capacity to decrease BOD5 and COD to 43.32% and 44.30%, respectively, at pH 5 for 32 hours, dairy wastes are thought to be highly biodegradable 40 . COD and BOD are criteria that demonstrate treatment efficacy based on elimination percentage.…”

“…The greatest outcomes were obtained with inoculum concentrations of 16% at pH 5 (reduction of BOD was 43.07%) and 8% at pH 6.5 (reduction of BOD was 37.93%). 40 My research demonstrated that the decrease of BOD and COD is greater in comparison.…”

A predictive growth model for Yarrowia lipolytica ATCC 9773 in wastewater

“…The principal substances in wastewater are oils, fats and long-chain fatty acids, which are contaminants of aquatic ecosystems (Becerra-Gutiérrez et al, 2015). Biological treatments have been employed as an alternative to decontaminate wastewater (González et al, 2012;Tarón-Dunoyer et al, 2020). Some yeasts are well-known for their ability to grow and decompose post-industrial wastes.…”