A predictive growth model for Yarrowia lipolytica ATCC 9773 in wastewater

Abstract

This study focuses on the development of a secondary model for Yarrowia lipolytica in a sewage treatment process. The raw data of Y. lipolytica growth were adjusted to the Buchanan model in order to obtain growth parameters such as initial count cells (Y0), maximum specific growth rate (μmax), latency phase (λ) and maximum cell population (Ymax). The µ values obtained at different pH levels (5.0 to 8.0) were used to build the secondary model based on a linear equation. The results showed a significant effect of pH on µmax values. The validation process of the developed models displays accuracy (Af) and bias factor (Bf) values close to one, while the values of root mean square error (RMSE) were low, confirming that such models can predict the growth of Y. lipolytica in dairy wastewater. This can be interesting to optimize sewage treatments that involve this kind of microorganism. Moreover, the dairy wastewater was a good substrate to support the Yarrowia lipolytica's growth and could be used to produce enzymes.

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“…The growth curve of P. aeruginosa growing in dairy wastewater shows three phases: (1) lag phase, (2) logarithmic phase and (3) stationary phase (Figure 1). Similar behavior has been observed for Y. lipolytica growing in synthetic and dairy wastewater (Tarón-Dunoyer et al, (2021). These findings show the capability of the microorganisms to use some compounds present in the wastewater as a source of carbon, nitrogen and energy.…”