Biodegradation of acrylamide by a novel isolate, Cupriavidus oxalaticus ICTDB921: Identification and characterization of the acrylamidase produced

Bedade, Dattatray K.

doi:10.1016/j.biortech.2018.04.012

Cited by 37 publications

(51 citation statements)

References 38 publications

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“…The results of this study show that the bacterium responsible for the degradation of acrylamide was able to make use of basic aliphatic amides, as was previously documented by other research [28,[41][42][43][44][45][46][47]. It is also unable of degrading 2chloroacetamide, an amide molecule that many degraders are unable to use.…”

Section: Discussionsupporting

confidence: 79%

Characterization of An Acrylamide-degrading Bacterium Isolated from Volcanic Soil

Rusnam

Gusmanizar

2022

J Environ Bioremed Toxicol

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Due to the fact that it breaks down into acrylamide over time, polyacrylamide is one of the most important sources of acrylamide in soil. As a strategy for bioremediation, the breakdown of acrylamide by the action of microbes has seen a gradual but consistent increase in attention all over the world. In this work, a bacterium, tentatively identified as Pseudomonas sp. strain Neni-12 that had been isolated from volcanic soil showed the ability to grow on acrylamide. The acrylamide-degrading bacterium grew best in the presence of glucose with acrylamide as the sole nitrogen source. At concentrations of acrylamide ranging from 400 to 600 mg/L, the organisms saw the greatest amount of growth, where ANOVA analysis shows no difference among these temperatures; however, growth was entirely halted at concentrations of 800 mg/L and above. The optimum pH was at 7.0, and growth was maximum between 25 and 35 °C. The bacterium is also capable of growing while using acetamide as the only source of nitrogen. An acrylamide-degrading bacterium that was isolated from volcanic soil is reported for the very first time here.

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Section: Discussionsupporting

confidence: 79%

Characterization of An Acrylamide-degrading Bacterium Isolated from Volcanic Soil

Rusnam

Gusmanizar

2022

J Environ Bioremed Toxicol

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“…This work demonstrates that the acrylamide-degrading consortium was able to utilise basic aliphatic amides, as previously described by other investigations [40][41][42][43][44][45][46][47]. Instead of several short-chain amides, it can also degrade 2chloroacetamide, an amide compound that cannot be utilized by Pseudomonas sp.…”

Section: Discussionsupporting

confidence: 78%

An Acrylamide-degrading Bacterial Consortium Isolated from Volcanic Soi

Rusnam

Gusmanizar

2021

J Biochem Microbiol Biotechnol

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In soil, polyacrylamide is a key source of acrylamide because it slowly decomposes into acrylamide. There has been a modest but steady rise in worldwide interest in microbe-mediated acrylamide decomposition as a bioremediation method. A bacterial consortium isolated from the volcanic soil of Mount Marapi, West Sumatra, Indonesia, was able to thrive on acrylamide in this study. Acrylamide-degrading bacteria grew best in the presence of 1 %(w/v) glucose with acrylamide as the sole nitrogen source. Optimum growth occurs in between 300 and 500 mg/L of acrylamide, pH between 6.5 and 8.0, and temperatures between 30 and 35 °C. The consortium can also grow on acetamide as the sole nitrogen source. Toxic heavy metals, such as mercury, silver and copper slowed down the growth of this consortium on acrylamide. This is the first report of an acrylamide-degrading consortium isolated from volcanic soils.

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“…The major foods contributors to acrylamide dietary exposure proved to be breakfast cereals and French fries. Acrylamide is formed by the Maillard reaction occurring between reducing sugars and proteins/amino acids at elevated temperatures [166,167]. Several factors related to food products composition have been shown to influence the acrylamide formation levels and temperatures [168,169].…”

Section: Food Applicationsmentioning

confidence: 99%

Recent Strategies and Applications for l-Asparaginase Confinement

et al. 2020

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l-asparaginase (ASNase, EC 3.5.1.1) is an aminohydrolase enzyme with important uses in the therapeutic/pharmaceutical and food industries. Its main applications are as an anticancer drug, mostly for acute lymphoblastic leukaemia (ALL) treatment, and in acrylamide reduction when starch-rich foods are cooked at temperatures above 100 °C. Its use as a biosensor for asparagine in both industries has also been reported. However, there are certain challenges associated with ASNase applications. Depending on the ASNase source, the major challenges of its pharmaceutical application are the hypersensitivity reactions that it causes in ALL patients and its short half-life and fast plasma clearance in the blood system by native proteases. In addition, ASNase is generally unstable and it is a thermolabile enzyme, which also hinders its application in the food sector. These drawbacks have been overcome by the ASNase confinement in different (nano)materials through distinct techniques, such as physical adsorption, covalent attachment and entrapment. Overall, this review describes the most recent strategies reported for ASNase confinement in numerous (nano)materials, highlighting its improved properties, especially specificity, half-life enhancement and thermal and operational stability improvement, allowing its reuse, increased proteolysis resistance and immunogenicity elimination. The most recent applications of confined ASNase in nanomaterials are reviewed for the first time, simultaneously providing prospects in the described fields of application.

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Biodegradation of acrylamide by a novel isolate, Cupriavidus oxalaticus ICTDB921: Identification and characterization of the acrylamidase produced

Cited by 37 publications

References 38 publications

Characterization of An Acrylamide-degrading Bacterium Isolated from Volcanic Soil

Characterization of An Acrylamide-degrading Bacterium Isolated from Volcanic Soil

An Acrylamide-degrading Bacterial Consortium Isolated from Volcanic Soi

Recent Strategies and Applications for l-Asparaginase Confinement

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