In soil, polyacrylamide is a key source of acrylamide because it slowly decomposes into acrylamide. There has been a modest but steady rise in worldwide interest in microbe-mediated acrylamide decomposition as a bioremediation method. A bacterial consortium isolated from the volcanic soil of Mount Marapi, West Sumatra, Indonesia, was able to thrive on acrylamide in this study. Acrylamide-degrading bacteria grew best in the presence of 1 %(w/v) glucose with acrylamide as the sole nitrogen source. Optimum growth occurs in between 300 and 500 mg/L of acrylamide, pH between 6.5 and 8.0, and temperatures between 30 and 35 °C. The consortium can also grow on acetamide as the sole nitrogen source. Toxic heavy metals, such as mercury, silver and copper slowed down the growth of this consortium on acrylamide. This is the first report of an acrylamide-degrading consortium isolated from volcanic soils.
Due to the fact that it breaks down into acrylamide over time, polyacrylamide is one of the most important sources of acrylamide in soil. As a strategy for bioremediation, the breakdown of acrylamide by the action of microbes has seen a gradual but consistent increase in attention all over the world. In this work, a bacterium, tentatively identified as Pseudomonas sp. strain Neni-12 that had been isolated from volcanic soil showed the ability to grow on acrylamide. The acrylamide-degrading bacterium grew best in the presence of glucose with acrylamide as the sole nitrogen source. At concentrations of acrylamide ranging from 400 to 600 mg/L, the organisms saw the greatest amount of growth, where ANOVA analysis shows no difference among these temperatures; however, growth was entirely halted at concentrations of 800 mg/L and above. The optimum pH was at 7.0, and growth was maximum between 25 and 35 °C. The bacterium is also capable of growing while using acetamide as the only source of nitrogen. An acrylamide-degrading bacterium that was isolated from volcanic soil is reported for the very first time here.
The inappropriate removal, manufacturing and prospecting actions and unnecessary use of agricultural chemical compounds have triggered an international issue. Eliminating these kinds of contaminants by means of bioremediation is a less expensive approach in the long term notably at low concentrations, in which various other approaches for instance physical or chemical approaches is probably not useful. In this work we screen the ability of a molybdenum-reducing bacterium isolated from polluted soil to make use of pesticides as electron donor sources for assisting reduction and as carbon sources for growth. The bacterium was not able to use pesticides as electron donors, nonetheless, the bacterium can grow on coumaphos separate from molybdenum reduction. Optimum conditions for Mo-blue production were at pH 6.3 and between 25 and 37 oC, glucose as electron donor, phosphate at 5.0 mM and sodium molybdate between 15 and 20 mM. Reduction was inhibited by Hg, Ag and Cr at 2 ppm by 91.9, 82.7 and 17.4 %, respectively. Biochemical analysis tentatively and partially identified the bacterium as Bacillus sp. strain Neni-12. This is a novel Mo-reducing bacterium with coumaphos degrading capability.
The introduction of tiny amounts of heavy metals into the environment can encourage the growth of a wide variety of microorganisms. The concentration at which enhanced microbial activity is seen, on the other hand, results in a significant decrease in growth rate as well as an increase in lag time (due to the higher lag time). An established link exists between heavy metal toxicity in microorganisms and the process of bioremediation, which has been well-documented. Because heavy metals have an impact on bioremediation, they must be researched, and appropriate countermeasures must be implemented. Copper reduced the growth of the SDS-degrading bacteria Enterobacter sp. strain Neni-13 to a significant extent. Under varying doses of mercury, the SDS-degrading bacteria exhibited a sigmoidal pattern with time periods ranging from 7 to 10 hours. Gompertz's model was used to calculate the growth rates of copper in different concentrations. As the copper concentration rose, the growth of bacteria was suppressed with a concentration of 1.0 g/L, with virtually total stoppage of bacterial development. From the Gompertz model, we got the estimates of growth rates; after which, they were estimated according to the Han-Levenspiel, Shukor, Wang, Liu, Andrews, and Amor models. The modified Han-Levenspiel, Andrews, Liu, and Shukor models could all successfully fit the curve. Results of the statistical analysis showed that the Han-Levenspiel model was the best model based on highest adjusted correlation coefficient (adR2), the lowest values for RMSE and AICc, and values of AF and BF closest to unity. The parameters obtained from the Han-Levenspiel model were Ccrit 0.209 mg/L (95%, C.I., 0.199 to 0.219), μmax 0.209 h-1 (95% C.I., 0.199 to 0.219) and m 0.472 (95% C.I., 0.383 to 0.561. The results obtained in this study indicate the maximum tolerable copper concentration that the conditions for biodegradation should not exceed.
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