Characterization of An Acrylamide-degrading Bacterium Isolated from Volcanic Soil

Abstract

Due to the fact that it breaks down into acrylamide over time, polyacrylamide is one of the most important sources of acrylamide in soil. As a strategy for bioremediation, the breakdown of acrylamide by the action of microbes has seen a gradual but consistent increase in attention all over the world. In this work, a bacterium, tentatively identified as Pseudomonas sp. strain Neni-12 that had been isolated from volcanic soil showed the ability to grow on acrylamide. The acrylamide-degrading bacterium grew best in the presence of glucose with acrylamide as the sole nitrogen source. At concentrations of acrylamide ranging from 400 to 600 mg/L, the organisms saw the greatest amount of growth, where ANOVA analysis shows no difference among these temperatures; however, growth was entirely halted at concentrations of 800 mg/L and above. The optimum pH was at 7.0, and growth was maximum between 25 and 35 °C. The bacterium is also capable of growing while using acetamide as the only source of nitrogen. An acrylamide-degrading bacterium that was isolated from volcanic soil is reported for the very first time here.

Characterization of the Growth of Pseudomonas sp. strain DrY135 on Acrylamide

“…Research examining the impact of pH on acrylamide development has yielded consistent results, as seen in the present study. Many microbes that break down acrylamide prefer an environment with a pH of about 7.0 [14][15][16][17][18][19][20][21][22][23]. Soils in tropical regions often have a lower pH because of the high levels of metabolic activity that produce organic acid and carbon dioxide.…”

“…A key factor in how quickly acrylamide is broken down by bacteria is temperature. Researchers have found that several bacteria capable of digesting acrylamide thrive at temperatures close to 30 degrees Celsius [14][15][16][17][18][19][20][21][22][23]. However, thermoactive bacteria, including Pseudonocardia thermophilic and Brevibacillus borstelensis BCS-1, require higher temperatures for optimum growth, with 50(C and 55(C being required, respectively [29,30].…”

“…Based on previous works, heavy metals have a significant impact on the breakdown of acrylamide, with greater inhibition occurring in the presence of mercury, copper and silver than other metals [15][16][17][18][20][21][22][23].In the published study that is available at this time, there is a dearth of knowledge concerning the effect that heavy metals have on the degradation of acrylamide and even other xenobiotics. This lack of information is due to the fact that the research has not yet been sufficiently conducted.…”

“…As acrylamide concentrations grew, the maximum growth rate also slowed, which suggests an overall tendency toward increasing toxicity. Acrylamide prevents the growth of a wide variety of bacteria, and doses of 1000 mg/L or more typically have this effect [15][16][17][18][20][21][22][23]. The enzyme amidase that is found in certain microorganisms makes it possible for them to grow at these significantly higher concentrations [19,[44][45][46][47][48][49][50].…”

“…Although acrylamide is quickly metabolized and eliminated from the body after exposure, it nevertheless poses a concern to workers and consumers [11][12][13] due to its high protein reactivity. Bacteria are still the most common type of microorganism shown to break down acrylamide [14][15][16][17][18][19][20][21][22][23]. Here we describe the isolation and characterization of another Pseudomonas acrylamide-degrading strain with metal-reducing capability.…”