Biocompatibility of a new nanomaterial based on calcium silicate implanted in subcutaneous connective tissue of rats

Petrović, Vera; Opacic-Galic, Vanja; Jokanović, Vukoman; Jovanović, M.; Basta-Jovanović, Gordana; Živković, Slavoljub

doi:10.2298/avb1206697p

Cited by 8 publications

(20 citation statements)

References 15 publications

(23 reference statements)

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“…e biocompatibility of ALBO-MPSA has been thoroughly studied and confirmed at in vitro and in vivo conditions and compared to MTA [14][15][16][17][18][19][20][21]. e aim of this paper was to further examine ALBO-MPSA biocompatibility, over the subchronic toxicity study.…”

Section: Introductionmentioning

confidence: 99%

Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates

Jokanovic

Čolović

Prokić

et al. 2018

Advances in Materials Science and Engineering

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As an alternative to MTA, a new endodontic material based on hydroxyapatite and calcium silicates (ALBO-MPSA) has been synthesized, and its biocompatibility has been studied in manyin vitroandin vivostudies. The current study aims to evaluate a subchronic systemic toxicity of ALBO-MPSA on the rat animal model, as a continuation of the previous studies. Biochemical parameters of blood and histological parameters of the liver, kidneys, and spleen of the rats were analyzed after 120 days of consumption of the aqueous extract of ALBO-MPSA. The results showed no myelotoxic effect or autoimmune effect on peripheral blood cells and no pathological effect on the liver, kidney, and spleen tissues. Besides, no changes in the skin and hair of the rats, neither the change in the consumption of food and water, nor the change in their usual behavior were noticed during the experiment.

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Section: Introductionmentioning

confidence: 99%

Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates

Jokanovic

Čolović

Prokić

et al. 2018

Advances in Materials Science and Engineering

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“…Finally, water was added to the mixture in a 1:2 ratio, to create cement paste. The second material used was calcium silicate (CS): 60% of the total quantity was β-C2S and C3S phase, with added components: 20% calcium carbonate (CaCO 3 ) and 20% BaSO 4 (Merck, Germany) [5,6,17] .…”

Section: Testing Materialsmentioning

confidence: 99%

“…In contact with biomaterial, tissue should not exhibit cytotoxic, genotoxic, mutagenic or allergic reactions [3][4][5] . If changes occur on the surrounding tissue, they are in the form of inflammation, necrosis, infiltration, encapsulation or some other reaction by the tissue [6] .…”

Section: Introductionmentioning

confidence: 99%

“…Some implanted biomaterials have a partially adverse impact on leukocytes in peripheral blood, in the form of the occurrence of toxic granules in the cytoplasm [7] . Many researchers have studied the biocompatibility of calcium silicate cements and, in the last decade, calcium aluminate cements [5,6,8,9] . There is a growing interest in nanomaterials since, among other things, they have the possibility to overcome the problems associated with solubility and stability, and to enable medicine to be released into the desired tissue [10] .…”

Section: Introductionmentioning

confidence: 99%

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Kalsiyum Alüminat ve Kalsiyum Silikat Sement İmplantasyonunun Sıçanlarda Hematolojik Profil Üzerine Etkileri

Katica¹,

Janković²,

Tandir³

et al. 2020

Kafkas Univ Vet Fak Derg

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The objective of this study was to evaluate potential unfavourable side effects, especially on the blood corpuscles, of experimental calcium aluminate and calcium silicate cements that are applied subdermally or directly to tooth pulp. In the study, fifty-four Wistar rats were separated into two study groups (n=27 in each group). The effects of the dental cements on haematological parameters were observed three times (after 7, 15 and 30 days): erythrocytes, haemoglobin, haematocrit, mean corpuscular volume, mean corpuscular haemoglobin, mean corpuscular haemoglobin concentration, leukocytes, leucogram and poikilocytic erythrocyte forms. There were no statistically significant differences in the total number of leukocytes and percentage results of lymphocytes, and neutrophils. The study recorded lower numbers of erythrocytes, with no connection with the type of material applied to the dental pulp (First study group). Significant differences in results for the second study group were recorded on days 7-15 and 15-30 of the experiment in relation to both types of implanted cements. In the second study group there were significant differences related to both types of implanted cements, in the same periods. Lower values of erythrocytes, haemoglobin and haematocrit indicated the existence of hypochromic anaemia caused by the damaging influence of calcium aluminate and calcium silicate. Research showed that in both study groups, normocytic hypochromic anaemia existed, along with modest to expressed distribution of anulocytes and stomatocytes. According to the results of the research, the negative impact of application of Ca-silicate was recorded and was related to the appearance of hypochromic anaemia with poikilocytic forms of erythrocytes.

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“…Die Herstellung der Huhnerembryofibroblastenund der Schweinenierenkulturen erfolgte nach den bereits mitgeteilten Methoden (1,2 T a b e l l e I V e r g l e i c h e n d e U n t e r s u c h u n g e n i i b e r d e n N a c h w e i s d e s A u j e s z k y -V i r u s a u s T o n s i l l e n , L u n g e n u n d G e h i r n e n n a t i i r l i c h i n f i z i e r t e r S c h w e i n e i i b e r Z e l l k u l t u r e n T a b e l l e 2 Q u a n t i t a t i v e r A u j e s z k y -V i r u s -N a c h w e i s a u s T o n s i l l e n , G e h i r n e n u n d L u n g e n n a t u r l i c h e r k r a n k t e r F e r k e l u n d L a u f c r u b e r Z e l l k u l t u r e n…”

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Die Bedeutung der Tonsillen für die Pathogenese und Diagnose der Aujeszkyschen Krankheit bei Schweinen

Masic

Ercegan

Petrović

1965

Zentralblatt für Veterinärmedizin Reihe B

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Zusammenfassung Durch vergleichende Untersuchungen prüften wir das Auftreten und die Intensität des zytopathogenen Effektes in Zellkulturen, die mit Tonsillen‐, Gehirn‐ und Lungenmaterial natürlich mit Aujeszky‐Viren infizierter Schweine beimpft worden waren. In 21 von 24 Fällen trat der zytopathogene Effekt in denjenigen Zellkulturen, die mit Tonsillensuspensionen beimpft worden waren, 24 Stunden p.i. auf. Die Zellzerstörung war hier eher und deutlicher sichtbar als in Kulturen, die mit Gehirn‐ bzw. Lungensuspensionen inokuliert worden waren. Nur in einem Fall zeigte sich der zytopathogene Effekt nach Verimpfung von Gehirnsuspension eher als nach Inokulation von Tonsillenmaterial. Bei 13 von 15 untersuchten Tieren lagen die Virustiter in den Tonsillen höher als in den Gehirnen bzw. Lungen der gleichen Schweine. In den Tonsillen schwankte der Virustiter zwischen 10−40 und 10−70 KID50, in den Gehirnen bzw. Lungen zwischen 0 und 10−5,50 KID50. Nur in einem Fall war der Titer in Tonsillen und Lungen gleich hoch. Unsere Untersuchungsergebnisse weisen darauf hin, daß sich das Virus bei natürlich infizierten Schweinen primär in den Tonsillen vermehrt und von dort aus in die Blutbahn eindringt. Im Verlauf einer Virämie gelangt es in das Zentralnervensystem, in die Lungen, den Uterus gravidis etc. In diesen Organen kann es pathologische Veränderungen hervorrufen. Wir halten es allerdings auch für möglich, daß das Virus nach intranasaler Infektion direkt auf dem Nervenweg das Zentralnervensystem erreicht. Für eine Virusvermehrung in den Tonsillen sprechen pathologische Veränderungen in Form verschieden großer nekrotischer Herde in den lymphatischen Follikeln. Unsere Befunde zeigen, daß zur Isolierung des Aujeszky‐Virus bei Schweinen neben Gehirn und Lungen besonders die Tonsillen herangezogen werden müssen. Dadurch gelangt man schneller zu einer sicheren Diagnose. Summary The significance of the tonsils for the pathogenesis and diagnosis of Aujeszky's disease in pigs In comparative studies the authors studied the development and intensity of the cytopathogenic effects (CPE) in cell cultures inoculated with tonsil, brain and lung material from pigs infected with Aujeszky virus. In 21 of 24 samples the CPE appeared in cultures inoculated with tonsil suspensions 24 hours previously. Cell damage was earlier and clearer than in cultures inoculated with brain or lung suspensions. Only on one occasion did the CPE occur earlier with brain suspension than with tonsil material. In 13 of 15 animals the viral titre in the tonsils was higher than in the brain or lung, the tonsil titres ranging between 10−4.0 and 10−7.0 KID50 and the levels in brain and lungs between 0 and 10−5,50 KID50. Only in one animal was the titre in the lungs as high as in the tonsils. These studies show that in naturally‐infected swine the virus multiplies primarily in the tonsils and from there reaches the bloodstream from which it invades the central nervous system, the lungs, and the gravid uterus. In these organs it can produce pathological changes. It is a...

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Biocompatibility of a new nanomaterial based on calcium silicate implanted in subcutaneous connective tissue of rats

Cited by 8 publications

References 15 publications

Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates

Subchronic Systemic Toxicity of New Endodontic Material Based on Calcium Hydroxyapatite and Calcium Silicates

Kalsiyum Alüminat ve Kalsiyum Silikat Sement İmplantasyonunun Sıçanlarda Hematolojik Profil Üzerine Etkileri

Die Bedeutung der Tonsillen für die Pathogenese und Diagnose der Aujeszkyschen Krankheit bei Schweinen

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