Biochemical difference between ventral and dorsolateral prostates: Effects of castration and administration of the antiandrogen 16β‐ethyl‐17β‐hydroxy‐4‐oestren‐3‐one on SDS‐electrophoretic patterns of subcellular proteins from rat prostates

Wada, Fumio; Nishi, Nozomu; Matuo, Yuhsi

doi:10.1002/pros.2990050306

Cited by 6 publications

(1 citation statement)

References 8 publications

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“…Effects of androgen with-drawal after orchiectomy or application of antiandrogens, and subsequent replacement of androgens, have been reviewed by Brandes (1974) and Aumuller (1979). The effects of androgens and withdrawal of androgens have been evaluated by means of histological and ultrastructural parameters (Kawamura and Ichihara, 1987;Timms and Chandler, 1983) and immunohistochemical (Aumuller and Seitz, 1990;Fawell and Higgins, 1986) and biochemical methods (Aumuller and Seitz, 1990;Fjosne et al, 1988;Harkonen, 1981Harkonen, , 1982Harkonen and Vaananen, 1988;Wada et al, 1984). Additionally to androgens, both estrogens and proteohormones have been shown to modulate the regulation of rat prostatic function.…”

Section: Effects Of Experimentalmentioning

confidence: 99%

Effects of hormones on the prostate in adult and aging men and animals

Sinowatz

Amselgruber

Plendl

et al. 1995

Microscopy Res & Technique

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Literature on the effect of steroid hormones (androgens, estrogens, and other steroids), of peptide hormones (e.g., prolactin), and growth factors (e.g., EGF, FGF, TGF-beta), on the effect of castration and of experimental hormone application on the prostate is reviewed. Androgens have inductive, repressive, and interactive effects. They counterbalance an agonistic effect on proliferation and an antagonistic effect on cell death; they may influence DNA synthesis and induce the synthesis of substances with mitogenic effects on the prostate. Estrogens exert direct and indirect effects on the prostate. They suppress the secretion of gonatropins, thus repressing testicular androgen secretion. They stimulate the fibromuscular stroma and induce squamous metaplasia of the epithelium. Estrogens may also be involved in the onset of benign prostatic hyperplasia. Prolactin is preferentially bound in the diseased human prostate. An abundance of information has been gained on EGF, FGF, TGF-beta, and other growth factors. They may be involved in the development of prostatic hyperplasia. Castration leads to a striking reduction in prostatic size in a short period of time due to autophagic and heterophagic processes. In castrated individuals, the prostate is enriched in androgen-independent cells. Experimental hormone application involves the substitution of androgens as well as anti-androgens, long-term application of different hormones, and application of combinations of drugs. The results of several studies are described. Further directions in the field of prostate research should concentrate on the role of growth factors in prostate development and pathology and on the effect of certain lectins on prostate diseases. We think that the investigation of interactions between steroid hormones and growth factors in normal and pathological neovascularization of the prostate is important.

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Section: Effects Of Experimentalmentioning

confidence: 99%

Effects of hormones on the prostate in adult and aging men and animals

Sinowatz

Amselgruber

Plendl

et al. 1995

Microscopy Res & Technique

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Comparison of subcellular proteins of normal prostate, benign prostatic hypertrophy, and prostatic cancer: Presence of BPH‐associated nonhistone proteins

et al. 1985

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Proteins in the cytosol, postnuclear particulate, and nuclear fractions from seven specimens of normal prostate from bladder cancer patients, 14 specimens of benign hypertrophic prostate (BPH), and three specimens of cancerous prostate were analyzed and compared by SDS-polyacrylamide slab gel electrophoresis. Abundant protein species in the cytosol fractions were 60K (species having a molecular weight of about 60,000) and 42K; their relative contents were about 35% for 60K and about 12% for 42K. In the postnuclear particulate fraction, 42K was the most abundant (about 10% of the total). The contents of these major protein species were similar in specimens of normal and diseased prostates. In addition, there are marked similarities in the electrophoretic patterns for all the protein (24-29 species) in the cytosol and postnuclear particulate fractions of the human prostate, except for four minor species in the cytosol fraction. Of the nuclear proteins, the content of core histones (H2A, H2B, H3, and H4) was fundamentally similar among all the specimens, whereas the content of H1 histone was different from one specimen to another. The most remarkable and significant difference was that the 42K-NHP (nonhistone protein having a molecular weight of about 42,000), 55K-NHP, and 190K-NHP concentrations were significantly higher in BPH than in normal and cancerous prostates.

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Changes of cytosol proteins in the ventral and dorsolateral prostates of rats during sexual maturation: Immature prostate‐abundant proteins as detected by polyacrylamide gel electrophoreses

Wada¹,

Nishi²,

Muguruma³

et al. 1985

The Prostate

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Cytosol proteins in the ventral and dorsolateral prostates of rats from four to 31 weeks of age were analyzed by SDS‐electrophoresis. By comparing the relative contents (% of the total protein), it was found that in addition to the known major proteins which became abundant with age, there were several species abundant in the sexually immature prostates: (1) Ventral prostate: two kinds of secretory proteins with apparent molecular weights of about 31,000 (31K) and 67,000 (67K) and five kinds of nonsecretory proteins (42K, 57K, 58K, 80K and 84K) were abundant at weeks 4‐5; the most susceptible species was 80K, whose content at week 4 decreased to about one tenth at week 31. (2) Dorsolateral prostate: a nonsecretory protein (41K) and 14.4K, 34K, 80K, and 85K, which were distributed into the secretory and nonsecretory fractions, were abundant at weeks 4‐7; the most susceptible species was 80K, whose content at week 4 decreased to about one tenth after week 11. Total contents (content/100 g body weight) of all the protein species increased by aging in either prostate; the most susceptible species were 13K in the ventral prostate and 120K in the dorsolateral prostate, whose total contents increased 90‐100 times during sexual maturation.

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Biochemical difference between ventral and dorsolateral prostates: Effects of castration and administration of the antiandrogen 16β‐ethyl‐17β‐hydroxy‐4‐oestren‐3‐one on SDS‐electrophoretic patterns of subcellular proteins from rat prostates

Cited by 6 publications

References 8 publications

Effects of hormones on the prostate in adult and aging men and animals

Effects of hormones on the prostate in adult and aging men and animals

Comparison of subcellular proteins of normal prostate, benign prostatic hypertrophy, and prostatic cancer: Presence of BPH‐associated nonhistone proteins

Changes of cytosol proteins in the ventral and dorsolateral prostates of rats during sexual maturation: Immature prostate‐abundant proteins as detected by polyacrylamide gel electrophoreses

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