Changes of cytosol proteins in the ventral and dorsolateral prostates of rats during sexual maturation: Immature prostate‐abundant proteins as detected by polyacrylamide gel electrophoreses

Abstract

Cytosol proteins in the ventral and dorsolateral prostates of rats from four to 31 weeks of age were analyzed by SDS‐electrophoresis. By comparing the relative contents (% of the total protein), it was found that in addition to the known major proteins which became abundant with age, there were several species abundant in the sexually immature prostates: (1) Ventral prostate: two kinds of secretory proteins with apparent molecular weights of about 31,000 (31K) and 67,000 (67K) and five kinds of nonsecretory proteins (42K, 57K, 58K, 80K and 84K) were abundant at weeks 4‐5; the most susceptible species was 80K, whose content at week 4 decreased to about one tenth at week 31. (2) Dorsolateral prostate: a nonsecretory protein (41K) and 14.4K, 34K, 80K, and 85K, which were distributed into the secretory and nonsecretory fractions, were abundant at weeks 4‐7; the most susceptible species was 80K, whose content at week 4 decreased to about one tenth after week 11. Total contents (content/100 g body weight) of all the protein species increased by aging in either prostate; the most susceptible species were 13K in the ventral prostate and 120K in the dorsolateral prostate, whose total contents increased 90‐100 times during sexual maturation.

Radioimmunoassay of mouse vas deferens protein: developmental profile and hormonal regulation

“…*P<005 compared with controls of the same age (Student's Mest). & Higgins, 1986) and prostate (Heyns et al 1978;Higgins et al 1982;Wada et al 1985) show similar marked changes as a function of age. In mouse pro¬ state (Mills et al 1987 ), seminal vesicle (Normand et al 1989) and epididymis (Lefrançois et al 1992), specific proteins are detectable in young animals (10-20 days) and begin to accumulate in large amounts between 20 and 40 days.…”

“…The synthesis and secretion of tissue-specific marker pro¬ teins during development have received limited atten¬ tion. The appearance of androgen-dependent proteins during the development of the seminal vesicle (Kistler et al 1981 ;Fawell & Higgins, 1986;Normand et al 1989), prostate (Heyns et al 1978 ;Higgins et al 1982 ;Wada et al 1985;Mills et al 1987è) and epididymis (Faye et al 1980;Kohane et al 1983;Brooks, 1987;Charest et al 1989;Lefrançois et al 1992) seems to be quite variable, and no report exists on the role of androgens in the expression of these proteins during sexual maturation.…”

Isolation of a 41 kilodalton cytosol protein from the dunning rat prostatic adenocarcinoma: Characterization as depolymerized actin isomers

“…Wilson and colleagues [4-61 reported that transferrin and albumin appear to be major cytosol proteins of the Dunning tumor, from which they succeeded in purifying transferrin. We have recently reported that the Dunning tumor resembles the dorsolateral prostate of immature rats in its low contents of zinc and "probasin, " an androgen-dependent protein abundantly localized in the dorsolateral prostate of mature rats [7,8], and that a 41,000-dalton protein was abundant in both the immature dorsolateral prostate and the Dunning tumor [7,9]. On the other hand, comparison of two-dimensional electrophoretic patterns between normal prostate and Dunning tumors suggests an irreversible change in the cytoskeletal protein in the tumor [3].…”