The potential usefulness of a zwitterionic detergent, 3-[(3-cholamidopropyl)dimethylammonio]-1-propane sulfonate (CHPAS), in the stabilization of acidic and basic fibroblast growth factors (FGFs) was examined. Among several detergents, CHAPS was found to be not only non-cytotoxic but also most useful in handling the diluted preparations of FGFs. The advantages are as follows: 1) at lower concentrations than 0.01% CHAPS did not affect growth factor activity of calf serum (CS) and the growth rate of BALB/c 3T3 cells. The primary culture of rat prostate epithelium and colony formation of NRK-49F cells were hardly influenced by CHAPS lower than 0.003%; 2) the loss of FGFs that usually occurs due to their adherence to the surface of storage containers was effectively prevented by inclusion of 0.1% CHAPS; 3) the recovery of FGFs after storage or dialysis was significantly enhanced by inclusion of 0.1% CHAPS; 4) CHAPS at lower concentrations than 0.1% does not interfere with amino acid analysis, except that Thr may be misled only when the ratio of protein/CHAPS is low; 5) amino acid sequence analysis was hardly disturbed by CHAPS up to 0.5%. These results indicate that CHAPS is useful as a stabilizing agent for various kinds of polypeptides capable of showing biological activity at a low concentration.
Cytosol proteins in the ventral and dorsolateral prostates of rats from four to 31 weeks of age were analyzed by SDS‐electrophoresis. By comparing the relative contents (% of the total protein), it was found that in addition to the known major proteins which became abundant with age, there were several species abundant in the sexually immature prostates: (1) Ventral prostate: two kinds of secretory proteins with apparent molecular weights of about 31,000 (31K) and 67,000 (67K) and five kinds of nonsecretory proteins (42K, 57K, 58K, 80K and 84K) were abundant at weeks 4‐5; the most susceptible species was 80K, whose content at week 4 decreased to about one tenth at week 31. (2) Dorsolateral prostate: a nonsecretory protein (41K) and 14.4K, 34K, 80K, and 85K, which were distributed into the secretory and nonsecretory fractions, were abundant at weeks 4‐7; the most susceptible species was 80K, whose content at week 4 decreased to about one tenth after week 11. Total contents (content/100 g body weight) of all the protein species increased by aging in either prostate; the most susceptible species were 13K in the ventral prostate and 120K in the dorsolateral prostate, whose total contents increased 90‐100 times during sexual maturation.
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