Comparison of subcellular proteins of normal prostate, benign prostatic hypertrophy, and prostatic cancer: Presence of BPH‐associated nonhistone proteins

Abstract

Proteins in the cytosol, postnuclear particulate, and nuclear fractions from seven specimens of normal prostate from bladder cancer patients, 14 specimens of benign hypertrophic prostate (BPH), and three specimens of cancerous prostate were analyzed and compared by SDS-polyacrylamide slab gel electrophoresis. Abundant protein species in the cytosol fractions were 60K (species having a molecular weight of about 60,000) and 42K; their relative contents were about 35% for 60K and about 12% for 42K. In the postnuclear particulate fraction, 42K was the most abundant (about 10% of the total). The contents of these major protein species were similar in specimens of normal and diseased prostates. In addition, there are marked similarities in the electrophoretic patterns for all the protein (24-29 species) in the cytosol and postnuclear particulate fractions of the human prostate, except for four minor species in the cytosol fraction. Of the nuclear proteins, the content of core histones (H2A, H2B, H3, and H4) was fundamentally similar among all the specimens, whereas the content of H1 histone was different from one specimen to another. The most remarkable and significant difference was that the 42K-NHP (nonhistone protein having a molecular weight of about 42,000), 55K-NHP, and 190K-NHP concentrations were significantly higher in BPH than in normal and cancerous prostates.

Protein Profiles of Benign Hypertrophic Prostate: Stroma-Abundant Distribution of Bph-Associated Nonhistone Proteins

“…We have been studying differences between androgen-dependent subcellular proteins in the ventral and dorsolateral prostates of rats using sodium dodecyl sulfate (SDS)-electrophoresis [ 12-17, 22, 261. Recently, we found the presence of BPH-associated nonhistone proteins by comparative analysis of subcellular proteins among normal, benign hypertrophic, and cancerous prostates using SDS-polyacrylamide slab gel electrophoresis [27]. The present paper deals with (1) protein profiles of epithelium and stroma mechanically isolated from benign hypertrophic prostates and (2) distribution of BPH-associated nonhistone proteins in the epithelium and stroma, using polyacrylamide gel electrophoresis.…”

“…We previously found three kinds of BPH-associated nonhistone proteins by comparative analysis of proteins in subcellular components isolated from whole tissues among normal, benign hypertrophic, and cancerous prostates using SDS-polyacrylamide gel electrophoresis [27]. To examine the distribution of the three kinds of BPH-associated nonhistone proteins, BPH-associated Nonhistone Proteins 51 nuclear fractions isolated from the epithelium and stroma were analyzed by SDSpolyacrylamide gel electrophoresis.…”