Bioactive pigment production by Pseudomonas spp. MCC 3145: Statistical media optimization, biochemical characterization, fungicidal and DNA intercalation-based cytostatic activity

Patil, Sandeep; Nikam, Monali; Patil, Hemangi; Anokhina, Tatyana S.; Кочетков, В. В.; Chaudhari, Ambalal

doi:10.1016/j.procbio.2017.05.003

Cited by 15 publications

(15 citation statements)

References 39 publications

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“…Pyocyanin production by P. aeruginosa is affected by environmental factors including carbon and nitrogen sources, incubation time, concentration of salts, availability of oxygen, temperature, and pH of the medium (Jayaseelan et al 2014;Patil et al 2017). It also determined that alkaline protease enhanced pyocyanin production (Liyama et al 2017).…”

Section: Introductionmentioning

confidence: 99%

A new strategy for the efficient production of pyocyanin, a versatile pigment, in Pseudomonas aeruginosa OG1 via toluene addition

Özdal

2019

3 Biotech

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Pseudomonas aeruginosa produce pyocyanin, which is an extracellular secondary metabolite and multifunctional pigment. In this study, the effects of several surfactants (Tween 20, Tween 80 and Triton X-100) and organic solvents (toluene and chloroform) on pyocyanin production and cell growth were investigated in submerged culture of P. aeruginosa OG1. Organic solvents were found to be more effective in the production of pyocyanin. The maximum production of pyocyanin (33 mg/L) was achieved when 0.2% toluene was added at the stationary growth phase (30 h), corresponding to significant increase of 312% compared with the control (8 mg/L). With the addition of toluene, pyocyanin production was significantly increased, but bacterial biomass reduced. Production of alkaline protease was also affected by toluene addition. It was found that the ratio of saturated/unsaturated fatty acids in the bacterial biomass significantly increased when toluene addition to the medium. This study revealed that with a novel strategy, the addition of toluene to the fermentation medium significantly increased pyocyanin production. These findings suggest that solvent-assisted fermentation strategy can be used in microbial fermentations to increase the production of biotechnological products such as industrially important pigment and enzyme. This study is a first investigation on the stimulation of pyocyanin release in the medium of P. aeruginosa cultures by the addition of toluene.

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Section: Introductionmentioning

confidence: 99%

A new strategy for the efficient production of pyocyanin, a versatile pigment, in Pseudomonas aeruginosa OG1 via toluene addition

Özdal

2019

3 Biotech

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“…According to these data, the pyocyanin pigment used in our study was shown to possess a similar activity potential with the substances already used as AChE-i. The pyocyanin pigment's low molecular weight and zwitterion property facilitate its passage through cell membranes in eukaryotic systems [70]. It is thought that the inhibitory activity of the pyocyanin pigment having 210 Da molecular weight on AChE enzyme activity results from a sufficiently small size to pass through the cell membrane and its redox active compound property [45].…”

Section: Discussionmentioning

confidence: 99%

Pyocyanin Isolated from Pseudomonas aeruginosa: Characterization, Biological Activity and Its Role in Cancer and Neurodegenerative Diseases

Koyun

Şirin

Erdem

et al. 2022

Braz. arch. biol. technol.

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This study aimed to find a pigment of bacterial origin with protective/preventive activity against neurological injury and carcinogenesis, which may be used as a natural food additive in food activity or an active drug ingredient of an anti-neural injury/anticancer agent in the pharmaceutical industry. Within this scope, the Pseudomonas aeruginosa MB713 strain was used to produce pyocyanin. Characterization of the pyocyanin was carried out using UV-VIS, FT-IR, and GC-MS analyses. In addition, its inhibitory action on AChE and antioxidant activities were determined. The cytotoxic activities of the pyocyanin as well as its antiproliferative activities were detected. It was determined that the maximum production of the pyocyanin (51 μg/mL). The pyocyanin did not cause a cell death rate above 50% in L929 cells. The pyocyanin was found to exert an inhibitory effect on the AChE. The pyocyanin was found effective on all antioxidant parameters tested. The IC50 values of the pyocyanin on SK-MEL-30 and HT-29 cells were calculated as 72 and 179 μM, respectively. And due to the pyocyanin's antioxidant and inhibitory effects on AChE, antiproliferative effects, and protective effects against neurodegenerative injury, the use of the pyocyanin as HIGHLIGHTS• The maximum production of the pyocyanin was found to occur in MFDM and with shaking.• UV-VIS spectroscopy, GC-MS, and FT-IR analyses showed the presence of pyocyanin.• Pyocyanin had no cytotoxic impact on L929 cells (except 50-100 μM concentrations).• Pyocyanin had an antiproliferative impact on SK-MEL-30 cells.• Pyocyanin had in vitro and cellular AChE inhibitory and antioxidant properties.• The use of the pyocyanin as a neuroprotective agent or an anticancer agent is advised.

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“…M-18Q and showed 65.3% increase in phenazine pigment yield as compare to the original medium. Patil et al [24] reported peptone as a significant variable in PBD for the production of bioactive pigment (pyocyanin) by Pseudomonas spp. MCC 3145, which is in agreement with our results.…”

Section: Optimization Of Significant Medium Components Using Response Surface Methodology (Rsm)mentioning

confidence: 99%

Characterization, application and statistical optimization approach for enhanced production of pyocyanin pigment by Pseudomonas aeruginosa DN9

Gahlout

Chauhan

Prajapati

et al. 2021

Syst Microbiol and Biomanuf

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The aim of this work was to study the pyocyanin pigment from Pseudomonas aeruginosa DN9. The work involves optimization of process parameters for enhanced production of pyocyanin pigment under submerged fermentation condition. During optimization process, maximum pyocyanin production (92.12 µg/ml) was obtained with carbon source mannitole, nitrogen source peptone, inorganic salt NaCl and metal ion FeSO 4 . Plackett Burman design and Response Surface Methodology (RSM) showed peptone, NaCl and KH 2 PO 4 are significant variable in the production of pyocyanin pigment. The FTIR and GC-MS study was done to evaluate structural properties of pyocyanin pigment. The purified pigment was further analyzed as colouring agent and for inhibitory action against pathogenic microorganisms. Thus, present study showed Pseudomonas aeruginosa DN9 as promising culture for pigment production with potential biotechnological application.

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Bioactive pigment production by Pseudomonas spp. MCC 3145: Statistical media optimization, biochemical characterization, fungicidal and DNA intercalation-based cytostatic activity

Cited by 15 publications

References 39 publications

A new strategy for the efficient production of pyocyanin, a versatile pigment, in Pseudomonas aeruginosa OG1 via toluene addition

A new strategy for the efficient production of pyocyanin, a versatile pigment, in Pseudomonas aeruginosa OG1 via toluene addition

Pyocyanin Isolated from Pseudomonas aeruginosa: Characterization, Biological Activity and Its Role in Cancer and Neurodegenerative Diseases

Characterization, application and statistical optimization approach for enhanced production of pyocyanin pigment by Pseudomonas aeruginosa DN9

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