Detergents (surface active agents) are chemical compounds which are largely used for washing purpose. In present time the use of detergent has increased in household and industries, which causes numerous problems in sewage treatment facilities and causes toxic effect on organisms and eco system. Present study reports the isolation, morphological and estimation of detergent degrading bacteria. Soil samples collected from various detergent contaminated region ( Vapi and Valsad Region). Detergent was used as a sole source of carbon to enumerate detergent degrader. Total 16 isolates (S1..... S16) were obtained from soil sample, using basal salt medium. S7 isolate as shows maximum percentage of degradation at 1% concentration of detergent. The analysis shows that isolated organism belongs to Klebsiella species.

Pseudomonas spp. produces large quantities of water soluble blue green phenazine pigment pyocyanin. The blue green chloroform soluble phenazine pigment extracted from Pseudomonas spp has antimicrobial activity. In present study a total of nine bacterial isolated screened from the different environment sample. The isolates obtained were gram negative, non-spore forming and exhibited fluorescence under UV light. The isolate showing blue green pigment on king's B agar medium at a temperature of 35ᵒC after 48-72 hr. of incubation and considered as having capabilities to produce pyocyanin.

The aim of this work was to study the pyocyanin pigment from Pseudomonas aeruginosa DN9. The work involves optimization of process parameters for enhanced production of pyocyanin pigment under submerged fermentation condition. During optimization process, maximum pyocyanin production (92.12 µg/ml) was obtained with carbon source mannitole, nitrogen source peptone, inorganic salt NaCl and metal ion FeSO 4 . Plackett Burman design and Response Surface Methodology (RSM) showed peptone, NaCl and KH 2 PO 4 are significant variable in the production of pyocyanin pigment. The FTIR and GC-MS study was done to evaluate structural properties of pyocyanin pigment. The purified pigment was further analyzed as colouring agent and for inhibitory action against pathogenic microorganisms. Thus, present study showed Pseudomonas aeruginosa DN9 as promising culture for pigment production with potential biotechnological application.

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Blue Green Algae are Prokaryotic, Oxygenic photosynthetic, Autotrophic and some of them are able to fix atmospheric nitrogen. Cyanobacteria used in aquaculture, wastewater treatment, food, fertilizers. Its play an important role in maintainance and build-up of soil fertility, consequently increasing cereals growth and yield as a natural biofertilizer. In the present study, total 25 water sample were taken from of various ponds, lakes and rivers in valsad, vapi and surat region. A total of 8 cyanobacteria among 25 sample were isolated. Cyanobacteria grows well on algal culture broth and urea broth. The incubation period taken was 15-20 days for the growth of the cyanobacteria by using light energy. In microscopic examination cyanobacterial strain like oscillatoriasps, Nostocsps, Anabaena sps, ,Gloeocapsasps, PlectonemaspsandGloeothecespsetc were identified. The isolated cyanobacteria were used for further study of bioremediation of waste water and chromium sorption.