A new strategy for the efficient production of pyocyanin, a versatile pigment, in Pseudomonas aeruginosa OG1 via toluene addition

Abstract

Pseudomonas aeruginosa produce pyocyanin, which is an extracellular secondary metabolite and multifunctional pigment. In this study, the effects of several surfactants (Tween 20, Tween 80 and Triton X-100) and organic solvents (toluene and chloroform) on pyocyanin production and cell growth were investigated in submerged culture of P. aeruginosa OG1. Organic solvents were found to be more effective in the production of pyocyanin. The maximum production of pyocyanin (33 mg/L) was achieved when 0.2% toluene was added at the stationary growth phase (30 h), corresponding to significant increase of 312% compared with the control (8 mg/L). With the addition of toluene, pyocyanin production was significantly increased, but bacterial biomass reduced. Production of alkaline protease was also affected by toluene addition. It was found that the ratio of saturated/unsaturated fatty acids in the bacterial biomass significantly increased when toluene addition to the medium. This study revealed that with a novel strategy, the addition of toluene to the fermentation medium significantly increased pyocyanin production. These findings suggest that solvent-assisted fermentation strategy can be used in microbial fermentations to increase the production of biotechnological products such as industrially important pigment and enzyme. This study is a first investigation on the stimulation of pyocyanin release in the medium of P. aeruginosa cultures by the addition of toluene.

The influence of nanomaterials on pyocyanin production by Pseudomonas aeruginosa

“…However, pyocyanin remains a costly reagent (e.g., €118 for 5 mg on Merck website). Therefore, the production of pyocyanin is optimized by many research groups by adjusting process conditions, genetic engineering of the microorganisms, or the addition of various substances to the medium, including nanomaterials (Sismaet et al 2014;El-Fouly et al 2015;Ozdal 2019;Ozdal et al 2019;Bacame-Valenzuela et al 2020;da Silva et al 2021;Elbargisy 2021). The analysis of the literature shows that most of the data concerning the influence of nanomaterials on pyocyanin production indicate the inhibition of pigment production.…”

Isolation and identification of the pyrethroid insecticide deltamethrin degrading bacteria from insects

“…Serratia marcescens MO-1 isolated from grasshopper (Poecilimon tauricola) has both chitinase activity (Okay et al, 2013) and the ability to produce prodigiosin pigment (Kurbanoglu et al, 2015) which has antimicrobial and anticancer properties. Also, Pseudomonas aeruginosa OG1 isolated from cockroaches (Blatta orientalis) can produce pyocyanin, a pigment of biotechnological importance (Ozdal, 2019). Insects can change their ecological and physiological properties thanks to symbiotic bacteria .…”

Bivariate OSMAC Designs Expand the Secondary Metabolite Production Space in Corallococcus Coralloides

“…The addition of organic solvents seems to be a field where a broader screening is reasonable. It has been reported that an addition of toluene increases the saturated fatty acid content in the bacterial membranes and, thus, leads to increased membrane rigidity [29]. Possibly, an altered membrane composition influenced the secretion of metabolites in C. coralloides.…”