The antioxidant activity of the water extract of Tilia argentea Desf ex DC was determined by the thiocyanate method. The antioxidant activity of the water extract increased with the increasing amount of lyophilized extract (50-400 microg) added into the linoleic acid emulsion. Statistically significant effect was determined in 100 microg and higher amounts. Antioxidant activities of water extracts of tilia (Tilia argentea Desf ex DC), sage (Salvia triloba L.), and two Turkish black teas commercially called Rize tea and young shoot tea (Camellia sinensis) were compared. For comparison studies, 100 microg portions of extracts were added into test samples. All samples were able to show statistically significant antioxidant effect. Both of the tea extracts showed highest antioxidant activities, nevertheless, differences between tilia and sage and tilia and tea were not statistically significant (for both cases p > 0.05). Like antioxidant activity, the reducing power of water extract of Tilia argentea Desf ex DC was also concentration dependent. Even in the presence of 50 microg of extract, the reducing power was significantly higher than that of the control (p < 0.05) in which there was no extract. Unlike antioxidant activity, the highest reducing power activity was shown by sage extract. Among the tea extracts, young shoot extract was the most effective one, however, it had significantly lower activity than sage (p < 0.05). Although tea flower had the lowest reducing power activity, it was higher than that of tilia. But this difference was not statistically significant (p > 0.05). From these results, we could suggest that although the reducing power of a substance may be an indicator of its potential antioxidant activity, there may not always be a linear correlation between these two activities. In addition, antimicrobial activities of each of the above extracts were studied by disk diffusion methods on different test microorganisms. None of the extracts showed antibacterial activity on the studied microorganisms.
This work addresses the production of prodigiosin from ram horn peptone (RHP)
using MO-1, a local isolate in submerged culture. First, a novel gram-negative
and rod-shaped bacterial strain, MO-1, was isolated from the body of the
grasshopper (Poecilemon tauricola Ramme 1951), which was
collected from pesticide-contaminated fields. Sequence analysis of 16S rDNA
classified the microbe as Serratia marcescens. The substrate
utilization potential (BIOLOG) and fatty acid methyl ester profile (FAME) of
S. marcescens were also determined. The effect of RHP on
the production of prodigiosin by S. marcescens MO-1 was
investigated, and the results showed that RHP supplementation promoted the
growth of MO-1 and increased the production of prodigiosin. A concentration of
0.4% (w/v) RHP resulted in the greatest yield of prodigiosin (277.74 mg/L) after
48 h when mannitol was used as the sole source of carbon. The pigment yield was
also influenced by the types of carbon sources and peptones. As a result, RHP
was demonstrated to be a suitable substrate for prodigiosin production. These
results revealed that prodigiosin could be produced efficiently by S.
marcescens using RHP.
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