Binding of caffeine, theophylline, and theobromine with human serum albumin: A spectroscopic study

Zhang, Hongmei; Chen, Tingting; Zhou, Qinqin; Wang, Yanqing

doi:10.1016/j.molstruc.2009.09.032

Cited by 56 publications

(24 citation statements)

References 49 publications

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“…19E) showed the conformation of the BSA changes in the presence of extracts. There are few publications on applications of 3D fluorescence spectra Zhang, Chen, Zhou, & Wang, 2009), therefore the conclusions that 3-D fluorescence can be used as an additional tool for the characterization of the polyphenol extracts during different stages of ripening and different exotic fruits cultivars correspond with the previous data (Gorinstein et al, 2011-this issue;Gorinstein et al, 2010). The results revealed that polyphenol extracts caused the fluorescence quenching of BSA by the formation of polyphenol-BSA complex.…”

Section: Snake Fruitsupporting

confidence: 82%

The multiple nutrition properties of some exotic fruits: Biological activity and active metabolites

Dembitsky

Poovarodom

Leontowicz

et al. 2011

Food Research International

280

130

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Section: Snake Fruitsupporting

confidence: 82%

The multiple nutrition properties of some exotic fruits: Biological activity and active metabolites

Dembitsky

Poovarodom

Leontowicz

et al. 2011

Food Research International

280

130

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“…Figure represents the far‐UV CD spectra BSA (5μM) in the absence and presence of 1:5 and 1:10 (Protein:Drug) molar ratio of TFB (25μM and 50μM). The CD spectrum of native protein, ie, BSA, shows 2 characteristic peaks at or around 222 (due to n → pi * transition) and 208 nm (due to pi → pi * transition), signifying the presence of α‐helical structure of BSA . Figure shows some alteration in the secondary structure of albumin upon interacting with TFB.…”

Section: Resultsmentioning

confidence: 92%

A biophysical and computational study unraveling the molecular interaction mechanism of a new Janus kinase inhibitor Tofacitinib with bovine serum albumin

Nusrat

Ajmal

Zakariya

et al. 2016

J of Molecular Recognition

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The interaction of a recently certified kinase inhibitor Tofacitinib (TFB) with bovine serum albumin (BSA) has been studied, by spectroscopic and molecular docking studies. Spectrofluorimetric measurements at 3 different temperatures (288, 298, and 310 K) showed that TFB quench the intrinsic fluorescence of BSA upon forming a nonfluorescent complex. The intrinsic fluorescence data showed that TFB binds to BSA with binding constant (K ) of approximately 10 M , affirming a significant affinity of TFB with BSA. The decrease in Stern-Volmer quenching constant with increasing temperature exhibited the static mechanism of quenching. Negative value of ΔG (-6.94 ± 0.32 kcal·mol ), ΔH (-7.87 ± 0.52 kcal·mol ), and ΔS (-3.14 ± 0.42 cal·mol ·K ) at all 3 temperatures declared the reaction between BSA and TFB to be spontaneous and exothermic. Far-UV circular dichroism spectroscopy results demonstrated an increase in helical content of BSA in the presence of TFB. Moreover, dynamic light scattering measurements showed that TFB resulted into a decrease in the hydrodynamic radii (from 3.6 ± 0.053 to 2.9 ± 0.02 nm) of BSA. Molecular docking studies confirmed that TFB binds near site II on BSA, hydrogen bonding, and hydrophobic interaction were involved in the BSA-TFB complex formation. The present study characterizing the BSA-TFB interaction could be significant towards gaining an insight into the drug pharmacokinetics and pharmacodynamics and also in the direction of rational drug designing with better competence, against emerging immune-mediated diseases, ie, alopecia and rheumatoid arthritis.

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“…Other results (Xiao et al ., ,b; Zhang et al ., ) differ from that reported by us, probably because of the variety of antioxidant abilities of pure flavonoids and different ranges of fluorometry scanning used in a similar study. Our in vitro results of interaction between HSA and quercetin can be compared with other reports (Zhang et al ., ). There are not too many applications of 3D fluorescence spectra; therefore, our present conclusions – that 3D fluorescence can be used as an additional tool for the characterisation of the polyphenol extracts of berries cultivars – correspond with the previous data (Gorinstein et al ., ) and can be applied to any food analysis.…”

Section: Resultsmentioning

confidence: 99%

“…This interaction between quercetin and HSA was investigated using tryptophan fluorescence quenching. Other results (Xiao et al ., ,b; Zhang et al ., ) differ from that reported by us, probably because of the variety of antioxidant abilities of pure flavonoids and different ranges of fluorometry scanning used in a similar study. Our in vitro results of interaction between HSA and quercetin can be compared with other reports (Zhang et al ., ).…”

Section: Resultsmentioning

confidence: 99%

Comparative assessment of two extraction procedures for determination of bioactive compounds in some berries used for daily food consumption

Namieśnik

Vearasilp

Leontowicz

et al. 2013

Int J of Food Sci Tech

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Summary Two extractions with methanol and water were used to determine the antioxidant and binding properties of some berries as a supplement to food. Fluorometry, FTIR spectra and radical scavenging assays were used for characterisation of bioactive compounds (polyphenols, flavonoids, flavanols and tannins) and the levels of their antioxidant activities (AAs). The contents of bioactive compounds and AAs in water and methanol polyphenol extracts in gooseberries, blueberries and cranberries differed, but not always significantly. Water extracts of gooseberries showed the lowest amounts of polyphenols (mg GAE g−1), 6.24 ± 0.6, and flavonoids (mg CE g−1), 0.29 ± 0.01, and AAs (μMTE g−1) determined by DPPH, FRAP, ABTS and CUPRAC assays such as 6.05 ± 0.6, 8.07 ± 0.9, 18.70 ± 1.8 and 13.44 ± 1.2, respectively, in comparison with blueberries and cranberries. Polyphenol content highly correlated with antioxidant activity (R2 from 0.94 to 0.81). The quenching properties of berries were studied by the interaction of water and methanol polyphenol extracts with HSA by 3D fluorescence. In conclusion, the bioactivity of gooseberries was lower than in blueberries and cranberries. Gooseberries can be used as a new source for food consumption and supplementation based on their antioxidant and binding properties. 3D fluorescence spectroscopy and FTIR spectroscopy can be applied as additional analytical tools for rapid estimation of the quality of different food products.

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Binding of caffeine, theophylline, and theobromine with human serum albumin: A spectroscopic study

Cited by 56 publications

References 49 publications

The multiple nutrition properties of some exotic fruits: Biological activity and active metabolites

The multiple nutrition properties of some exotic fruits: Biological activity and active metabolites

A biophysical and computational study unraveling the molecular interaction mechanism of a new Janus kinase inhibitor Tofacitinib with bovine serum albumin

Comparative assessment of two extraction procedures for determination of bioactive compounds in some berries used for daily food consumption

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