Comparative assessment of two extraction procedures for determination of bioactive compounds in some berries used for daily food consumption

Abstract

Summary Two extractions with methanol and water were used to determine the antioxidant and binding properties of some berries as a supplement to food. Fluorometry, FTIR spectra and radical scavenging assays were used for characterisation of bioactive compounds (polyphenols, flavonoids, flavanols and tannins) and the levels of their antioxidant activities (AAs). The contents of bioactive compounds and AAs in water and methanol polyphenol extracts in gooseberries, blueberries and cranberries differed, but not always significantly. Water extracts of gooseberries showed the lowest amounts of polyphenols (mg GAE g−1), 6.24 ± 0.6, and flavonoids (mg CE g−1), 0.29 ± 0.01, and AAs (μMTE g−1) determined by DPPH, FRAP, ABTS and CUPRAC assays such as 6.05 ± 0.6, 8.07 ± 0.9, 18.70 ± 1.8 and 13.44 ± 1.2, respectively, in comparison with blueberries and cranberries. Polyphenol content highly correlated with antioxidant activity (R2 from 0.94 to 0.81). The quenching properties of berries were studied by the interaction of water and methanol polyphenol extracts with HSA by 3D fluorescence. In conclusion, the bioactivity of gooseberries was lower than in blueberries and cranberries. Gooseberries can be used as a new source for food consumption and supplementation based on their antioxidant and binding properties. 3D fluorescence spectroscopy and FTIR spectroscopy can be applied as additional analytical tools for rapid estimation of the quality of different food products.

Comprehensive two-dimensional gas chromatography and three-dimensional fluorometry for detection of volatile and bioactive substances in some berries

“…Polyphenols (mg GAE g À 1 DW) in water extracts of gooseberries and blueberries were 6.2470.6 and 57.4774.2 and the TAC (mM TE g À 1 DW) determined by ABTS and CUPRAC assays were for gooseberries and blueberries, respectively [12]. The results shown in Table 2 slightly differ from the results reported previously [2,12], depending on the solvent used, its ratio to the dry material and also on the time and type of extraction. The optimization of ultrasound operational parameters (solvent polarity, sample particle size), according to a specific plant matrix (leaves, flowers, stems) is also of main importance in order to achieve high extraction efficiency [23].…”

“…The efficacy of the individual antioxidants and the efficacy of a combination of two antioxidants were measured using fluorescence spectroscopy and showed a synergistic effect. Our very recent results [1,2,12,13] showed that the fluorescence is significantly quenched, because of the conformation of proteins, phenolic acids and flavonoids. Our result is in agreement with others that quercetin, as an aglycon, is more hydrophobic and demonstrates strong affinity toward HSA.…”

“…Berries are consumed because of their attractive color, special taste and aroma, and are considered one of the richest sources of natural antioxidants [1,2]. Aroma is one of the most important attributes that affects the flavor of a fruit and thus its acceptability and consumption [3].…”

Recent patents on the application of bioactive compounds in food: a short review

“…Important factors in the process are solvent type, extraction time and temperature [9]. Solvents or co solvents with different polarities such as ethanol and water [5,10,11] and/or organic solvents [4,6,7] can be employed for solid-liquid extractions in heat-reflux systems. Furthermore, non-conventional methods (ultrasound, pulsed electric field, enzyme digestion, extrusion, microwave heating, ohmic heating, supercritical fluids) are more environment friendly due to decreased use of synthetic and organic chemicals and reduced operational time, enhancing extract yield and quality [9].…”

Exploring the potential of an Andean fruit : an interdisciplinary study on the cape gooseberry (Physalis peruviana L.) value chain

“…However, from samples after heat treatment , we could only quantify catechin and epicatechin. Previous research identified quercetin, rutin, myricetin, epicatechin, catechin and kaempferol in cape gooseberry [121,122,[116][117][118]124] and although we replicated the procedures of extraction, hydrolysis (if required) and analysis, we could not come up with the same results. We therefore limit the discussion to catechin and epicatechin; the fate of these compounds is presented in figure 4.5.…”