Bifidobacterium from infant stool: the diversity and potential screening

Kusharyati, Dyah Fitri; Pramono, Hendro; Ryandini, Dini; Manshur, Tsani Abu; Dewi, Meilany Ariati; Khatimah, Khusnul; Rovik, Anwar

doi:10.13057/biodiv/d210623

Cited by 6 publications

(8 citation statements)

References 40 publications

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“…Gram, capsule, flagella and spore staining; also, biochemical tests such as catalase, oxidase, gelatin hydrolysis, gas, indole, motility in sulfide indole motility (SIM) medium, nitrate reduction, oxidative‐fermentative, urease and Simmon citrate assays were used to identify the isolates in the colonies (Kusharyati et al . 2020).…”

Section: Methodsmentioning

confidence: 99%

“…Fermentation of carbohydrates was evaluated with bromothymol blue reagent, and the media were yellow (weakly acidic solution) or blue (weakly base solution) (Kusharyati et al . 2020).…”

Section: Methodsmentioning

confidence: 99%

“…The growth turbidity of Bifidobacterium was measured with a spectrophotometer at 620 nm (Kusharyati et al . 2020; Nath et al . 2020).…”

Section: Methodsmentioning

confidence: 99%

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Synergistic effects of probiotic Bifidobacterium isolated from chicken's intestine in combination with polyvinylpyrrolidone on reduction of aflatoxin B1

Aalipanah

Fazeli

Sepahi

et al. 2022

Letters in Applied Microbiology

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Significance and Impact of the Study: Although various technologies (physical, chemical and biological) have been investigated to reduce the adverse effects of aflatoxins, these efforts have not yet resulted in a cost-effective, general toxin binder to eliminate or reduce aflatoxins. The present study results show that Bifidobacterium bifidum, a native probiotic isolated from chick's intestines in combination with PVP polymer, has a high ability (up to 90%) in removing AFB 1 from MRS medium and, therefore, can be used as a toxin binder for future studies.

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Section: Methodsmentioning

confidence: 99%

“…Fermentation of carbohydrates was evaluated with bromothymol blue reagent, and the media were yellow (weakly acidic solution) or blue (weakly base solution) (Kusharyati et al . 2020).…”

Section: Methodsmentioning

confidence: 99%

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Synergistic effects of probiotic Bifidobacterium isolated from chicken's intestine in combination with polyvinylpyrrolidone on reduction of aflatoxin B1

Aalipanah

Fazeli

Sepahi

et al. 2022

Letters in Applied Microbiology

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“…LAB are commonly isolated from fermented food products, milk, and the intestinal tract of humans or animals (Dobson et al 2012;Kusharyati et al 2020). Meanwhile, only a few studies isolated LAB from soils, water, and plants.…”

Section: Introductionmentioning

confidence: 99%

Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product Preservation

Kusharyati

Satwika

Mariana

et al. 2021

J.Tropical Biodiversity Biotechnology

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The meat and fisheries products have high nutritional content which is highly ideal for bacterial growth. Lactic Acid Bacteria (LAB) have several potential advantages as a bio-preservative agent in the food industry because they produce antimicrobial substances against pathogenic bacteria e.g. bacteriocin. Our previous study has succeeded in isolating and characterizing LAB from the mangrove sediments of Logending Beach, Kebumen. This present study aimed to determine the activity of bacteriocinogenic-LAB against food-borne pathogens and their potential for fisheries product preservation. The study consisted of five serial stages, as follows: screening of LAB isolates, cell-free supernatant production and its inhibition activity, extraction of partially purified bacteriocin, bacteriocin confirmation against proteolytic enzymes, and in-vitro test of partially-purified bacteriocin against Listeria monocytogenes, Shigella flexneri, and Salmonella typhi. A total of 25 out of 99 isolates were able to grow on MRSA+1% CaCO3 medium. Initial screening showed that the cell-free supernatant of 14 LAB isolates was able to inhibit the growth of S. thypi, S. flexneri, and L. monocytogenes. There was an increased inhibitory activity of partially purified bacteriocin when compared with the cell-free supernatant which was statistically different (p<0.01). It indicated that the purification was successfully performed. Bacteriocin expressed a lower inhibition against S. typhi than L. monocytogenes and S. flexneri. The ANOVA test showed that each indicator pathogenic-bacterium expresses a very significant sensitivity to the partially purified bacteriocin.

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“…Lactobacilli and Bifidobacteria have prebiotic properties (Guri et al 2015). They prefer oligosaccharides to be consumed and have immunomodulatory effects (Ding et al 2017;Kusharyati et al 2020). Mare's milk also contains biologically active compounds; lysozyme; lactoferrin; the vitamins A, B1, B2, B6, B9, B12, and C; and the trace elements Na, Ca, K, P, Fe, Mg, Cu, I, S, Si, Zn, and C (Kozhakhmetov et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Effect of mare’s milk prebiotic supplementation on the gut microbiome and the immune system following antibiotic therapy

et al. 2020

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Abstract. Nurgaziyev M, Atenov Y, Khassenbekova Z, Akpanova S, Rysbekov K, Kozhakhmetov S, Nurgozhina A, Sergazy S, Chulenbayeva L, Ospanova Z, Tuyakova A, Mukhambetganov N, Sattybayeva R, Urazova S, Galymgozhina N, Zhumadilova A, Gulyaev A, Kushugulova A. 2020. Effect of mare’s milk prebiotic supplementation on the gut microbiome and the immune system following antibiotic therapy. Biodiversitas 21: 5065-5071. Antibiotic treatment can severely affect the gut microbiome for short-term and long-term consequences. Probiotic and prebiotic supplements are widely prescribed to modulate the composition and function of the human gut microbiome. The current study aims to determine the impacts of mare’s milk prebiotics on the diversity of gut bacterial communities and the local immune system when administered during and after a course of antibiotic therapy. Six children aged 4 to 5 years diagnosed with bilateral bronchopneumonia were prescribed cephalosporin (cefuroxime) antibiotics. During the 60 days of the study, three children consumed mare’s milk prebiotics, while the other three did not. Fecal samples were collected daily during antibiotic therapy and every five days after the last day of antibiotic treatment. Total DNA was isolated, and the taxonomic composition of the gut microbiome was analyzed by sequencing the 16S rRNA gene (V1-V3 region). The MULTIPLEX MAP platform was used to evaluate the local immune status. The relative abundance of 11 genera was reduced and did not recover until the last day of the study. The abundance of Bacteroides was not significantly altered in either group. Christensenella, Rothia, Abiotrophia, Acinetobacter, Anaerotruncus, Holdemania, and Turicibacter numbers were significantly increased on day five and remained at the same level during the study period. Cephalosporin administration also reduced the levels of pro-inflammatory and anti-inflammatory cytokines/chemokines (MIP1α, TNFα, GMCSF, GCSF, sCD40L, FGF2, TGFα, IL1α, and IP10).

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Bifidobacterium from infant stool: the diversity and potential screening

Cited by 6 publications

References 40 publications

Synergistic effects of probiotic Bifidobacterium isolated from chicken's intestine in combination with polyvinylpyrrolidone on reduction of aflatoxin B1

Synergistic effects of probiotic Bifidobacterium isolated from chicken's intestine in combination with polyvinylpyrrolidone on reduction of aflatoxin B1

Potential Screening of Bacteriocinogenic-Lactic Acid Bacteria from Mangrove Sediment of Logending Beach for Fisheries Product Preservation

Effect of mare’s milk prebiotic supplementation on the gut microbiome and the immune system following antibiotic therapy

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