Bestimmung von Antikörpern gegen Cytomegalievirus (CMV), Varizella/Zoster-Virus (VZV) und Herpes simplex-Virus Typ 1 (HSV-1) im Enzyme-Linked-Immunosorbent-Assay (ELISA)

Ziegelmaier, R; Schneider, H; Hilfenhaus, J.; Thierfelder, H.; Behrens, Frank; Luthardt, Th.

doi:10.1007/bf00482264

Cited by 3 publications

(2 citation statements)

References 2 publications

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“…importance. Antigens prepared from CMV-infected cell suspensions, the most commonly used antigen in CF tests and in previously reported RIA [Knez et al, 19761 and ELISA tests [Gerna et al, 1977[Gerna et al, , 1978Cappel et al, 1978;Ziegelmaier et al, 1978; P h a and Pejtsik, 19791 produced high background values, unsatisfactory specific binding, and titration curves with inverted slope in IgG assays. A possible explanation for this may be CMV-induced Fc-binding, which is supported by the facts that the control antigen gave markedly less cpm than cell-associated CMV antigen when used in titrations of negative reference sera in IgG assays and that the inverted slope was not seen with the control antigen.…”

Section: Dlscusslonmentioning

confidence: 99%

“…Detection of cytomegalovirus (CMV)-specific antibodies by enzyme-linked immunosorbent assay (ELISA) and solid-phase radioimmunoassay (RIA) has been described by several authors [Knez et al, 1976;Gerna et al, 1977Gerna et al, , 1978Cappel et al, 1978;Ziegelmaier [Sarov et al, 19801 of these studies, antigens prepared from infected cell suspensions were used. Among possible pitfalls in CMV serology are false-positives due t o CMV-induced IgG-(Fc) receptors [Furukawa et al, 1975;Betts et al, 1976;Keller et al, 1976;Rahman et al, 1976;Griffiths et al, 19781, anticell antibodies [The and Langenhuysen, 1972;Anderson and Andersen, 19781 and rheumatoid factors [Gispen et al, 1975;Knez et al, 1976;Meurman et al, 1977;Cremer et al, 1978;Krishna et al, 19801, The present report describes a radioimmunoassay in which extracellular CMV harvested by ultracentrifugation was adsorbed on polystyrene beads and antibodies that reacted with the virus antigen were assayed by iodinated anti-human IgG, IgM, and IgA.…”

Section: Introductionmentioning

confidence: 99%

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Solid‐Phase Radioimmunoassay of Serum IgG, IgM, and IgA Antibodies to Cytomegalovirus

Torfason

Källander

Halonen

1981

Journal of Medical Virology

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A radioimmunoassay (RIA) using polystyrene beads as the solid phase for cytomegalovirus (CMV) antigen and iodinated immunosorbent purified anti-human IgG, IgM, and IgA as indicator antibodies was developed for the detection of immunoglobulin class-specific antibodies to CMV. An antigen prepared from extracellular virus was essential for reliable results, and a preparation ultracentrifuged and sonicated twice was better than a crude antigen. The optimal antigen gave low cpm values with a negative reference serum, resulting in cpm ratios of 10 or higher between early convalescent phase serum and negative reference serum. Of six patients with an increase in CMV CF titres, all six had an increase in RIA IgG titres, four had an increase in IgA titres, and all had IgM antibodies. The IgG titres were high, up to 1/64,000. In a group of 17 infants negative in CMV CF test, 14 had CMV IgG antibodies in RIA test, indicating mainly low levels of maternal antibodies. In six of seven patients with CMV isolations from urine specimens, an increase in IgG or IgA titres or the presence of IgM antibodies was found, and only one of these patients had an increase in CMV CF titre. The specificity of the developed CMV RIA test was further demonstrated by detecting no significant increase in RIA titres in serum specimens of patients with primary herpes simplex infection, chickenpox, herpes zoster, or infectious mononucleosis.

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Section: Dlscusslonmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Solid‐Phase Radioimmunoassay of Serum IgG, IgM, and IgA Antibodies to Cytomegalovirus

Torfason

Källander

Halonen

1981

Journal of Medical Virology

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Class-specific determination of antibodies against cytomegalo (CMV) and rubella virus by ELISA

Ziegelmaier

Behrens

Enders³

1981

Journal of Biological Standardization

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Correlation of herpes simplex virus antibody titers and specific lymphocyte stimulation in adult blood donors

Moser¹,

Behrens²,

Ziegelmaier³

et al. 1981

J Clin Microbiol

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Antibody titers to herpes simplex virus type 1 in sera from healthy adult donors were assayed by complement fixation, microneutralization, and an enzyme immunoassay (ELISA). This last test proved to be the most sensitive method for antibody detection. It was estimated that ELISA antibody titers were up to 40fold higher than neutralizing antibody titers and up to 100-fold higher than complement fixation antibody titers. Due to the higher sensitivity of ELISA, only 3 of 36 blood donors tested in this assay were shown to be seronegative, whereas 6 additional persons of the same group were termed seronegative by the microneutralization assay. Furthermore, four of the latter also did not respond in the complement fixation test. In vitro stimulation of peripheral lymphocytes by using a partially purified herpes simplex virus type 1 particle antigen was achieved for all seropositive blood donors. Only those three donors who were ELISA negative reacted negatively in this stimulation assay. From these results it may be concluded that ELISA is an appropriate method not only for rapid and sensitive antibody determination but also for selecting herpes simplex virus-negative patients.

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Bestimmung von Antikörpern gegen Cytomegalievirus (CMV), Varizella/Zoster-Virus (VZV) und Herpes simplex-Virus Typ 1 (HSV-1) im Enzyme-Linked-Immunosorbent-Assay (ELISA)

Cited by 3 publications

References 2 publications

Solid‐Phase Radioimmunoassay of Serum IgG, IgM, and IgA Antibodies to Cytomegalovirus

Solid‐Phase Radioimmunoassay of Serum IgG, IgM, and IgA Antibodies to Cytomegalovirus

Class-specific determination of antibodies against cytomegalo (CMV) and rubella virus by ELISA

Correlation of herpes simplex virus antibody titers and specific lymphocyte stimulation in adult blood donors

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