Class-specific determination of antibodies against cytomegalo (CMV) and rubella virus by ELISA

Ziegelmaier, R; Behrens, Frank; Enders, Gisela

doi:10.1016/s0092-1157(81)80062-5

Cited by 14 publications

(10 citation statements)

References 12 publications

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“…The earliest of these assays used infected cells as a solid phase and immunofluorescent reagents to detect IgM but were plagued with nonspecific reactions due to rheumatoid factor (RF) and Fc receptors on the surface of virus-infected cells (16). Assays that use rubella virus antigen on a solid phase (capture antigen) have been previously reported (17,24,33,36), using antibodies labeled with radioisotopes or enzymes. Assays that use antibodies to IgM on the solid phase (capture antibody) have been previously described, employing rubella virus antigen which is followed by an indicator system, such as rubella antiserum and erythrocytes (solid-phase immunosorbent technique) (10,18,30) or enzyme or radioactive probes (4,11,15,27,31).…”

mentioning

confidence: 99%

Clinical evaluation of the sensitivity and specificity of a commercially available enzyme immunoassay for detection of rubella virus-specific immunoglobulin M

Chernesky¹,

Wyman²,

Mahony³

et al. 1984

J Clin Microbiol

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A solid-phase capture antigen enzyme immunoassay (Rubazyme-M) was evaluated for sensitivity and specificity on sera from 1,200 blood donors, 51 patients with rubella, 2 infants with congenital rubella, 104 patients with other infections, and 126 patients with immunological abnormalities. The sensitivity was 100% for sera tested between days 3 and 40 after the onset of symptoms of rubella virus infection. Rubella virusspecific immunoglobulin M was detected at birth in sera from congenitally infected infants and persisted for several months. Positive Rubazyme-M responses were observed in some patients in the absence of rubella diagnosis (one blood donor, three other infections, and two immunological abnormalities), providing a test specificity of 99.6%. None of 67 patients with rubella virus-specific immunoglobulin G antibody and high levels of rheumatoid factor were positive in the test.

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mentioning

confidence: 99%

Clinical evaluation of the sensitivity and specificity of a commercially available enzyme immunoassay for detection of rubella virus-specific immunoglobulin M

Chernesky¹,

Wyman²,

Mahony³

et al. 1984

J Clin Microbiol

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“…However, the test is time-consuming, labor intensive, and difficult to standardize between laboratories. Several other antibody assays have recently been developed and tested, including radioimmunoassay (14), passive hemagglutination (3), single radial hemolysis in gel (5, 6), indirect immunofluorescence (5, 7), enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G (IgG) and IgM (8,11,21,22,26), and latex agglutination (LA) (13,24). These tests are rapid, simple, and usually require no pretreatment of sera; some now are available commercially in kit form.…”

mentioning

confidence: 99%

Use of enzyme immunoassays and the latex agglutination test to measure the temporal appearance of immunoglobulin G and M antibodies after natural infection or immunization with rubella virus

Meegan¹,

Evans²,

Horstmann³

1983

J Clin Microbiol

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The time course of appearance of antibodies after infection with rubella virus was determined with an immunoglobulin G (IgG) detection enzyme-linked immunosorbent assay, a latex agglutination test, and an IgM detection enzyme-linked immunosorbent assay. In six naturally infected rubella patients and 26 vaccinees, antibodies measured by either the IgG enzyme-linked immunosorbent assay or the latex agglutination test generally appeared in parallel with those detected by the hemagglutination inhibition test. By 28 days after inoculation of live virus vaccine and by 2 days postonset of clinical rubella symptoms caused by natural infection, antibodies were found by the two tests for all individuals. A commercially available enzyme-linked immunosorbent assay kit was used to detect rubella-specific IgM. After natural infection, IgM appeared earlier than IgG, and although IgM titers decreased rapidly postinfection, in four of five patients antibodies were still detectable 40 to 43 days after the onset of clinical symptoms. After vaccine-induced infection, rubella-specific IgM was lower in titer than after natural infection and was detected in only three of seven vaccinees 70 days post-immunization.

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“…Quantitative determination of complement con sumption (anticomplementary activity, ACA) was per Antibody titers against cytomegalovirus, measles, mumps, rubella, and varicella zoster were measured using an ELISA technique [19,20]. Classical precipitin analysis was carried out as described by Eisen et al [21].…”

Section: Methodsmentioning

confidence: 99%

S-Sulfonation: a Reversible Chemical Modification of Human Immunoglobulins Permitting Intravenous Application

Gronski¹,

Hofstaetter²,

Kanzy³

et al. 1983

Vox Sanguinis

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S-sulfonation represents a reversible chemical modification of disulfide bonds by which under the special conditions chosen only about 2.2 cystine units per IgG molecule are cleaved. Physicochemical and functional evidence for reconstititution is presented. Molecules reconstituted in vitro or in vivo regain, within a few hours, a reactivity (antigen binding, immunoprecipitation, Clq-mediated cross-linking of immune complexes) comparable to equimolar control preparations.

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Class-specific determination of antibodies against cytomegalo (CMV) and rubella virus by ELISA

Cited by 14 publications

References 12 publications

Clinical evaluation of the sensitivity and specificity of a commercially available enzyme immunoassay for detection of rubella virus-specific immunoglobulin M

Clinical evaluation of the sensitivity and specificity of a commercially available enzyme immunoassay for detection of rubella virus-specific immunoglobulin M

Use of enzyme immunoassays and the latex agglutination test to measure the temporal appearance of immunoglobulin G and M antibodies after natural infection or immunization with rubella virus

S-Sulfonation: a Reversible Chemical Modification of Human Immunoglobulins Permitting Intravenous Application

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