Correlation of herpes simplex virus antibody titers and specific lymphocyte stimulation in adult blood donors

Moser, Hans; Behrens, Frank; Ziegelmaier, R; Hilfenhaus, J.; Mauler, R.

doi:10.1128/jcm.13.1.36-41.1981

Cited by 5 publications

References 26 publications

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Escherichia coli-derived envelope protein gD but not gC antigens of herpes simplex virus protect mice against a lethal challenge with HSV-1 and HSV-2

Bröker

Abel

Köhler

et al. 1990

Med Microbiol Immunol

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Immunization studies with HSV-1 and HSV-2 envelope proteins expressed in Escherichia coli were performed. After active immunization of mice with a gD-1 antigen (Leu53-Ala312) expressed as a fusion protein, the animals were protected from a lethal challenge with HSV-1 and HSV-2. In addition, antisera from rabbits immunized with the same gD-1 antigen also conferred passive immunity to mice against a challenge infection with either HSV-1 or HSV-2. In contrast to these successful gD-1 protection experiments, various gC-1 and gC-2 fusion proteins from E. coli failed to induce protective immunity. Moreover, the mice sera from immunized animals were not able to react with the authentic, glycosylated gC-1 and gC-2 envelope proteins, whereas sera raised against authentic gC-1 and gC-2 glycoproteins do recognize the gC fusion proteins from E. coli. These results indicate, that E. coli might represent an ideal system for expressing gD antigens as a possible component of a HSV vaccine, whereas gC antigen cannot be produced in an immunocompetent form in E. coli.

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Escherichia coli-derived envelope protein gD but not gC antigens of herpes simplex virus protect mice against a lethal challenge with HSV-1 and HSV-2

Bröker

Abel

Köhler

et al. 1990

Med Microbiol Immunol

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Protectivity of herpes simplex virus antigens: Studies in mice on the adjuvant effect of PICLC and on the dependence of protection on T cell competence

Hilfenhaus

Christ

Köhler

et al. 1981

Med Microbiol Immunol

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The efficacy of a herpes simplex virus type 1 (HSV-1) envelope antigen (EAG) preparation against HSV infection was studied in T cell competent and T cell deficient mice. Immuno-competent mice were successfully protected against herpes simplex virus type 1 (HSV-1) or type 2 (HSV-2) infection when immunized 2 weeks prior to this infection with a heat-inactivated whole virus preparation or a HSV-1 envelope antigen (HSV-1 EAG) preparation. Since HSV-1 EAG was considerably less effective than the whole virus preparation, a poly.riboinosinic-poly.ribocytidylic acid complex with poly-L-lysine and carboxymethylcellulose (PICLC) was used as adjuvant. Immunization with HSV-1 EAG plus PICLC resulted in a pronounced increase of this protection rate as compared with that obtained after immunization solely with HSV-1 EAG. PICLC alone, however, offered no protection when given 2 weeks before challenge. In T cell deficient nu/nu mice no protection was achieved with HSV-1 EAG while their T cell competent, heterozygous littermates were protected. From these results it may be concluded that T cell competence is a prerequisite for establishing a protective immunity against HSV infection after immunization with HSV-1 EAG.

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Immunobiology of Infection with Human Cytomegalovirus

Kirchner¹

1983

Advances in Cancer Research

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Correlation of herpes simplex virus antibody titers and specific lymphocyte stimulation in adult blood donors

Cited by 5 publications

References 26 publications

Escherichia coli-derived envelope protein gD but not gC antigens of herpes simplex virus protect mice against a lethal challenge with HSV-1 and HSV-2

Escherichia coli-derived envelope protein gD but not gC antigens of herpes simplex virus protect mice against a lethal challenge with HSV-1 and HSV-2

Protectivity of herpes simplex virus antigens: Studies in mice on the adjuvant effect of PICLC and on the dependence of protection on T cell competence

Immunobiology of Infection with Human Cytomegalovirus

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