Bacteriology, Inflammation, and Healing

Sibbald, R. Gary; Contreras‐Ruiz, José; Coutts, Patricia; Fierheller, Marjorie; Rothman, Arthur I.; Woo, Kevin

doi:10.1097/01.asw.0000294757.05049.85

Cited by 130 publications

(30 citation statements)

References 52 publications

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“…Previous studies have reported that silver ions and silver nanoparticles exert anti-inflammatory effects, induce lymphoproliferation, and inhibit bacterial and HIV-1 infection. These characteristics make silver nanoparticles of interest in microbicide research [48,49]. …”

Section: Discussionmentioning

confidence: 99%

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PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture

et al. 2010

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BackgroundPrevious in vitro studies have demonstrated that polyvinylpyrrolidone coated silver nanoparticles (PVP-coated AgNPs) have antiviral activity against HIV-1 at non-cytotoxic concentrations. These particles also demonstrate broad spectrum virucidal activity by preventing the interaction of HIV-1 gp120 and cellular CD4, thereby inhibiting fusion or entry of the virus into the host cell. In this study, we evaluated the antiviral activity of PVP-coated AgNPs as a potential topical vaginal microbicide to prevent transmission of HIV-1 infection using human cervical culture, an in vitro model that simulates in vivo conditions.ResultsWhen formulated into a non-spermicidal gel (Replens) at a concentration of 0.15 mg/mL, PVP-coated AgNPs prevented the transmission of cell-associated HIV-1 and cell-free HIV-1 isolates. Importantly, PVP-coated AgNPs were not toxic to the explant, even when the cervical tissues were exposed continuously to 0.15 mg/mL of PVP-coated AgNPs for 48 h. Only 1 min of PVP-coated AgNPs pretreatment to the explant was required to prevent transmission of HIV-1. Pre-treatment of the cervical explant with 0.15 mg/mL PVP-coated AgNPs for 20 min followed by extensive washing prevented the transmission of HIV-1 in this model for 48 h.ConclusionsA formulation of PVP-coated AgNPs homogenized in Replens gel acts rapidly to inhibit HIV-1 transmission after 1 min and offers long-lasting protection of the cervical tissue from infection for 48 h, with no evidence of cytotoxicity observed in the explants.Based on this data, PVP-coated AgNPs are a promising microbicidal candidate for use in topical vaginal/cervical agents to prevent HIV-1 transmission, and further research is warranted.

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Section: Discussionmentioning

confidence: 99%

“…Finally, previous studies report that silver nanoparticles and silver nanocrystals suppress the expression of TNF-α, which is a cytokine that plays a pivotal role in HIV-1 pathogenesis by up-regulating the transcription of HIV-1 [48,49]. It also prevents inflammation and, as such, may enhance wound healing in vivo [54].…”

Section: Discussionmentioning

confidence: 99%

PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture

et al. 2010

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“…In a human clinical study, wound dressing containing NSPs promoted the healing of chronic leg ulcers by not only reducing bacteria numbers in the wound bed, but by decreasing inflammatory response as well [25]. Nymark et al [26] prepared polyvinylpyrrolidone-coated silver nanoparticles and studied Genotoxicity in BEAS 2B cells.…”

Section: Introductionmentioning

confidence: 98%

Chitosan-PVP-nano silver oxide wound dressing: In vitro and in vivo evaluation

Archana

Singh

Dutta

et al. 2015

International Journal of Biological Macromolecules

312

134

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“…It is widely held that nanomaterials induce cytokine signal transduction, leading to a range of in vitro studies focused on the advantages of AgNPs for providing an anti-inflammatory effect and their role in wound healing by lowering the concentrations of inflammatory mediators such as interleukin 1 and interferons [8, 9, and 10]. Several researchers have investigated the effects of AgNPs on the bacterial wall and how AgNPs inhibit bacterial growth [11, 12, and 13].…”

Section: Introductionmentioning

confidence: 99%

Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α

Fehaid

Taniguchi

2018

Science and Technology of Advanced Materials

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Silver nanoparticles (AgNPs) are widely known to have anti-inflammatory properties, but the exact mechanism underlying this anti-inflammatory effect is not clearly understood. Tumor necrosis factor-α (TNFα) is a major pro-inflammatory cytokine that is expressed in the early stage of cell inflammation and induces apoptosis by several known pathways. Our study aimed to investigate the effect of AgNPs on the response of lung epithelial cells to TNFα and the molecular mechanism of this response. Lung epithelial cell line NCI-H292 cells were exposed to AgNPs (5 µg/mL) and/or TNFα (20 ng/mL) for 24 h, then cellular uptake was analyzed using flow cytometry. Our results showed that AgNPs were taken up by cells in a dose-dependent manner and that the cellular uptake ratio of AgNPs was significantly increased in the presence of TNFα. Apoptosis assays indicated that exposure to AgNPs significantly decreased the apoptotic effect of TNFα. Confocal microscopy was used to localize the tumor necrosis factor receptor 1 (TNFR1) and revealed that TNFR1 localized on the surface of cells exposed to TNFα. In contrast, TNFR1 localized inside cells exposed to both AgNPs and TNFα, with very few receptors scattered on the cell membrane. The results indicated that AgNPs reduced the cell surface TNFR1 expression level. The results suggested that the reduction of surface TNFR1 reduced cellular response to TNFα, resulting in an anti-apoptotic effect.

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Bacteriology, Inflammation, and Healing

Cited by 130 publications

References 52 publications

PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture

PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture

Chitosan-PVP-nano silver oxide wound dressing: In vitro and in vivo evaluation

Silver nanoparticles reduce the apoptosis induced by tumor necrosis factor-α

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