PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture

Lara, Humberto H; Ixtepan-Turrent, Liliana; Garza-Treviño, Elsa N.; Rodríguez‐Padilla, Cristina

doi:10.1186/1477-3155-8-15

Cited by 162 publications

(114 citation statements)

References 70 publications

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“…Notably, Lara et al demonstrated that a high concentration of polyvinyl pyrolidone-coated Ag-NPs (0.6 mg/mL) were only cytotoxic to a small population of cells, affecting the viability of 20% of the cells in the cervical explants. 30 In addition, our data show that Ag-NPs were stably bound to the polyurethane condom and were not significantly leached from the condom due to the interaction of the Ag-NPs with the nitrogen atom of the PUC. This feature could provide additional advantage for the product used at the mucosal surface since the Ag-NPs will be removed with the PUC after use.…”

Section: Discussionmentioning

confidence: 68%

“…The same amounts of human cervical cancer cells (HeLa cells) were exposed to the Ag-NPs-coated PUC or noncoated PUC for different times (10,30, and 180 minutes). Then, the Ag-NPs-coated PUC or noncoated PUC was removed and the growth of HeLa cells was monitored at different days postexposure by using a cell proliferation (WST-1) assay.…”

Section: Toxicity Of Ag-nps-coated Pucmentioning

confidence: 99%

See 1 more Smart Citation

Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection

Fayaz¹,

Ao²,

Girilal³

et al. 2012

IJN

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Recent research suggests that today's condoms are only 85% effective in preventing human immunodeficiency virus (HIV) and other sexually transmitted diseases. In response, there has been a push to develop more effective ways of decreasing the spread of the disease. The new nanotechnology-based condom holds the promise of being more potent than the first-generation products. The preliminary goal of this study was to develop a silver nanoparticles (Ag-NPs)-coated polyurethane condom (PUC) and to investigate its antimicrobial potential including the inactivation of HIV and herpes simplex virus (HSV) infectiousness. The Ag-NPs-coated PUC was characterized by using ultraviolet-visible spectrophotometry, Fourier transform-infrared spectroscopy, high-resolution scanning electron microscopy, and energy-dispersive analysis of X-ray spectroscopy. Nanoparticles were stable on the PUC and not washed away by water. Morphology of the PUC was retained after coating. The NP binding is due to its interaction with the nitrogen atom of the PUC. No significant toxic effects was observed when human HeLa cells, 293T and C8166 T cells were contacted to Ag-NPs-coated PUC for three hours. Interestingly, our results demonstrated that the contact of the Ag-NPs-coated PUC with HIV-1 and HSV-1/2 was able to efficiently inactivate their infectiousness. In an attempt to elucidate the antiviral action of the Ag-NPs, we have demonstrated that the anti-HIV activity was primarily mediated by the Ag-NPs, which are associated with the PUC. In addition, the data showed that both macrophage (M)-tropic and T lymphocyte (T)-tropic strains of HIV-1 were highly sensitive to the Ag-NPs-coated PUC. Furthermore, we also showed that the Ag-NPs-coated PUC was able to inhibit the growth of bacteria and fungi. These results demonstrated that the Ag-NPs-coated PUC is able to directly inactivate the microbe's infectious ability and provides another defense line against these sexually transmitted microbial infections.

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Section: Discussionmentioning

confidence: 68%

Section: Toxicity Of Ag-nps-coated Pucmentioning

confidence: 99%

Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection

Fayaz¹,

Ao²,

Girilal³

et al. 2012

IJN

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“…Hence, the silver-ZnO NC have acted upon the bacterial cell wall of E. coli, inhibiting its synthesis. According to Lara et al (2010a), bactericidal action based on the inhibition of cell wall synthesis could be another possible mechanism. This was further supported by the hypothesis that the bacteria following nano treatments become non-culturable due to inhibition of further cell division.…”

Section: Discussionmentioning

confidence: 99%

“…Nanoscale materials have emerged * Corresponding author. Email: a_b_das@hotmail.com as novel antimicrobial agents owing to their effectiveness in small doses, minimal toxicity and lack of side effects (Lara et al 2010(Lara et al , 2010a. Ag ions and Ag-based compounds are highly toxic to microorganisms, showing strong biocidal effects on as many as 12 species of bacteria including Escherichia coli (Zhao & Stevens 1998).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial effect of silver zinc oxide (Ag-ZnO) nanocomposite particles

Ghosh

Das

Jena

et al. 2014

Frontiers in Life Science

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The antimicrobial effects of silver nanocomposite particles (Ag-ZnO NC) on microorganisms, the antimicrobial mechanism and applications in medical devices are not yet clear. Stable Ag-ZnO NC were prepared and their morphological sizes and shapes were characterized by scanning electron microscopy. The effect of Ag-ZnO NC was tested on Bacillus thuringiensis, Escherichia coli and Pseudomonas aeruginosa in antibacterial tests including growth kinetics, antimicrobial susceptibility (disc diffusion) and minimal inhibitory concentration (MIC). Different concentrations of nanocomposites (i.e. 10, 20, 50,100, and 200 µg) showed concentration-dependant efficacy on all three tested microorganisms. E. coli was fairly sensitive in 200 µg of NC, forming a ∼15 mm inhibition zone; followed by B. thuringiensis, having ∼9 mm of inhibition zone, while P. aeruginosa, a pathogenic bacterium, showed negligible inhibition zone with Ag-ZnO NC. Growth of E. coli under Ag-ZnO NC treatment was significantly delayed with an extended lag phase of 2 hrs and 30 mins. Scanning electron microscopy confirmed the bacteriostatic effect of Ag-ZnO NC, which was manifested in cell division arrest with significant cell elongations compared to the control. The free radical generation effect of Ag-ZnO NC was tested against all these organisms. The results suggest that Ag-ZnO NC can be used effectively against microbial growth. This may be of use in diverse medical devices for antimicrobial control and can be a proper substitute for antibiotics in curing human health.

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“…This is an important finding because the use of siRNA has been effective in treating ocular diseases 18 and nanoparticles have been determined to be safe for mucosal tissue delivery Moreover, the use of nanoparticles for microbicidal delivery to both the female reproductive tract and the rectal mucosa indicates the potential for this approach to target primary HIV-1 infection in mucosal tissues. 12,[50][51][52] The potential to knock down gene expression within the female reproductive tract and induce protection against a sexually transmitted disease could be developed not only for HIV-1, but also for other pathogens that utilize cell-specific receptors for infection. Thus, it is possible to engineer nanoparticles for the delivery of siRNA, either alone or in combination with anti-HIV drugs, to achieve protection against sexual HIV-1 infection.…”

Section: Articlementioning

confidence: 99%

Nanoparticles Containing siRNA to Silence CD4 and CCR5 Reduce Expression of These Receptors and Inhibit HIV-1 Infection in Human Female Reproductive Tract Tissue Explants

Eszterhas

Ilonzo

Crozier

et al. 2011

Infectious Disease Reports

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Human Immunodeficiency Virus-type 1 (HIV-1) binds to CD4 and CCR5 receptors on target cells in the human female reproductive tract. We sought to determine whether reducing levels of messenger RNA (mRNA) transcripts that encode these receptors in female reproductive tract cells could protect mucosal tissue explants from HIV-1 infection. Explants prepared from the endometrium, endocervix, and ectocervix of hysterectomy tissues from HIV-1 sero-negative women were exposed to nanoparticles containing CD4-and CCR5-specific short-interfering RNA (siRNA) sequences. Explants were then exposed two days later to HIV-1, and HIV-1 reverse transcripts were measured five days post-infection. Explants treated with nanoparticles containing CD4-and CCR5-specific siRNA showed reduced levels of CD4 and CCR5 transcripts, and significantly lower levels of HIV-1 reverse transcripts compared to those treated with an irrelevant siRNA. In female reproductive tract explants and in peripheral blood cell cultures, siRNA transfection induced the secretion of IFN-alpha (IFN-α), a potent antiviral cytokine. In female mice, murine-specific Cd4-siRNA nanoparticles instilled within the uterus significantly reduced murine Cd4 transcripts by day 3. Our findings demonstrate that siRNA nanoparticles reduce expression of HIV-1 infectivity receptors in human female reproductive tract tissues and also inhibit HIV-1 infection. Murine studies demonstrate that nanoparticles can penetrate the reproductive tract tissues in vivo and silence gene expression. The induction of IFN-α after siRNA transfection can potentially contribute to the antiviral effect. These findings support the therapeutic development of nanoparticles to deliver siRNA molecules to silence host cell receptors in the female reproductive tract as a novel microbicide to inhibit mucosal HIV-1 transmission.

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PVP-coated silver nanoparticles block the transmission of cell-free and cell-associated HIV-1 in human cervical culture

Cited by 162 publications

References 70 publications

Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection

Inactivation of microbial infectiousness by silver nanoparticles-coated condom: a new approach to inhibit HIV- and HSV-transmitted infection

Antimicrobial effect of silver zinc oxide (Ag-ZnO) nanocomposite particles

Nanoparticles Containing siRNA to Silence CD4 and CCR5 Reduce Expression of These Receptors and Inhibit HIV-1 Infection in Human Female Reproductive Tract Tissue Explants

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