Bacterial Assay of Contact Lens Wearers

Hart, Dean E.; Hosmer, Marion E.; Géorgescu, Mihai; Farris, R. Linsy

doi:10.1097/00006324-199603000-00014

Cited by 14 publications

(10 citation statements)

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“…15 This is in agreement with another study that measured 17–64 cfu per conjunctival swab in 10% of the swabs, 5–16 cfu/swab in 15 % of the swabs, and as few as 0–4 cfu/swab in 75% of the swabs, illustrating the measurable but infrequent bacterial presence in the conjunctival tissues. 16 In contrast, lid swabs yielded a high number of cfu: 101–1000 cfu/swab in 3% of the cases, 11–100 cfu/swab in 38% of the swabs, and finally 0–10 cfu/swab in 59% of the swabs. 17 Cumulatively, these experiments demonstrated that there is a limited abundance of the in vitro sustainable microbial species at the ocular surfaces, including the lid margin areas, which is remarkably different from what is present in the oral mucosa or saliva.18–21 Consistently, 100% of the swabs taken from the oral mucosa and saliva yielded bacterial presence and contained 10 7 –10 8 cfu/ml of sustainable bacterial sp.…”

Section: Is There a Core Ocular Commensal Microbiota?mentioning

confidence: 96%

Impact of Microbiome on Ocular Health

2016

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The ocular surface is continuously exposed to the environment and, therefore, it is surprising that it harbors only few commensals with low degree of diversity. This unique aspect of the ocular surface physiology prompts the question whether there are core ocular commensal communities and how they affect ocular immunity. The purpose of this review is to provide an overview of what is known about the ocular surface commensals in health and disease and what we would like to learn in the near future. In addition, we discuss how microbiota at sites other than the eye may influence ocular immune responses. The information discussed in the review has been gathered using PubMed searches for literature published from January 1982 to December 2015.

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Section: Is There a Core Ocular Commensal Microbiota?mentioning

confidence: 96%

Impact of Microbiome on Ocular Health

2016

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“…There are multiple possibilities that explain these relationships. The conjunctiva or lid may serve as the source of contamination to the lens, Hart et al [11], Wilcox et al [12]. Contact lens wear may disrupt the ecology of the ocular surface by altering the balance of the normal micro biota.…”

Section: Organismmentioning

confidence: 99%

Detection of Biofilm Forming Ability of Bacterial Isolates from Contact Lenses and their Accessories

Zuberi¹,

Nadeem²

2017

J Bacteriol Parasitol

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In modern era the usage of contact lens is increased in youngsters especially in females with a high risk of ocular infections. In current research bacteriological analysis and bio-film forming ability of 22 contact lens accessories samples (Contact lenses, contact lens storage kit and contact lens solution) is studied. The isolated bacteria includes Corynebacterium spp, S. aureus, coagulase negative Staphylococcus, Bacillus spp. and Streptococcus spp. Bio-film are the main cause of bacterial infection. The bio-film forming ability of these bacterial isolates is studied by Congo red agar method and tube method. Best results were observed in Test tube method. The results initiate to continue further researches on the pathogenesis of bacteria as well as guide patients with proper contact lens procedures will hopefully decrease the incidence of ocular infections. Modern approaches for bio-film prevention and control will help in elimination of different diseases. The main objective of the research is to study the contact lens accessories isolated pathogens and their bio-film forming ability.

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“…12–18 To date, microorganisms that colonize lid margins13, 17, hands14, 16, lens cases12, 18, and the domestic water supply17 have been implicated as such sources. Furthermore, other studies have looked for associations between lens contamination and physical lens properties or patient factors 19–24.…”

mentioning

confidence: 99%

Risk Factors for Contact Lens Bacterial Contamination During Continuous Wear

Szczotka-Flynn

Bajaksouzian²,

Jacobs³

et al. 2009

Optometry and Vision Science

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Purpose Microbial contamination of contact lenses is associated with corneal infection and inflammation. This study determined which microbiological, clinical and demographic factors are associated with bacterial contamination of a silicone hydrogel contact lens when worn for continuous wear. Methods Two hundred five normal subjects were enrolled in the Longitudinal Analysis of Silicone Hydrogel (LASH) Contact Lens Study and were fitted with lotrafilcon A lenses for monthly continuous wear and followed for 1 year. Lenses were aseptically removed after 1 week and 4 months of wear and cultured using an agar sandwich technique. Lids and conjunctiva were routinely cultured at baseline, and after 1 week and 4 months of continuous wear. Lenses and ocular sites were considered to have substantial microbial bioburden when they harbored pathogenic organisms or high levels of commensal organisms. Univariate and multivariate logistic regression analyses were conducted to examine whether substantial conjunctival or lid bioburden, subject demographics, lens wearing history, symptoms, and biomicroscopic signs were associated with lens bioburden. Results About one-third (32.4%) of subjects had substantial bacterial bioburden in either eye across multiple visits. Over half (53.2%) and about one-tenth (11.7%) of subjects had substantial lid and conjunctival bioburden, respectively, and 11.2% discontinued due to discomfort. The adjusted odds ratios (and 95% confidence intervals) for presence of substantial lens bioburden were 2.49 (1.17–5.30), 4.24 (1.45–12.40), and 4.11 (1.17–14.46) for substantial lid bioburden, substantial conjunctival bioburden, and lens discomfort, respectively. Conclusions Bacterial contamination of silicone hydrogel contact lenses is common during continuous wear. Substantial lens bioburden is associated with discomfort precluding successful continuous wear. The presence of substantial lid and conjunctival bioburden are associated with a 2.5 fold and over 4 fold greater risk of substantial lens bioburden and are likely the major routes of contamination.

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Bacterial Assay of Contact Lens Wearers

Cited by 14 publications

References 0 publications

Impact of Microbiome on Ocular Health

Impact of Microbiome on Ocular Health

Detection of Biofilm Forming Ability of Bacterial Isolates from Contact Lenses and their Accessories

Risk Factors for Contact Lens Bacterial Contamination During Continuous Wear

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