Detection of Biofilm Forming Ability of Bacterial Isolates from Contact Lenses and their Accessories

Zuberi, Sameen Naz; Nadeem, Sayyeda Ghufrana

doi:10.4172/2155-9597.1000321

Cited by 3 publications

(4 citation statements)

References 9 publications

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“…Since all the three isolates originated from the (controlled) biofouling model, this signifies that biofilm formation could depend strongly on environmental conditions such as availability of nutrients, moisture and temperature. The result of Zuberi and Nadeem (2017) corroborates the finding of this study as they similarly reported as high as 63.64% biofilm formation in bacteria isolated from contact lenses and their accessories in Karachi, Pakistan.…”

Section: Resultssupporting

confidence: 91%

Rosmarinus officinalis Might be Exploited as a Natural Antifouling Agent: A Potentially Promising Strategy for Curbing Membrane Biofouling

Salisu

Umar

Kawo

et al. 2022

JSPAE

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Membrane biofouling is the coverage of membrane surfaces due to undesirable development of biofilms causing a decrease and subsequent loss of productivity in water treatment settings. Continuous use of synthetic chemicals against biofouling is inept as it leads to the emergence of multi-antibiotic resistance. Application of natural products such as plants can be apt in curbing biofouling while checking the resistance challenge. This study aimed to evaluate the potential of Rosmarinus officinalis in the control of membrane biofouling. Bacteria from biofouling environments were subjected to a biofilm confirmation test and identified at cultural, morphological, biochemical and molecular levels. Leaves of R.officinalis were extracted in solvents of varying polarity and activities. These extracts were evaluated against bacterial biofilm formation via minimum biofilm inhibitory concentration (MBIC), minimum biofilm eradication concentration (MBEC) and mesocosm bioassays. Biofilm formation was confirmed in 68% of the isolates identified as Pseudomonas aeruginosa, Klebsiella pneumoniae and Staphylococcus aureus. The methanol and ethyl acetate extracts of R.officinalis indicated the least MICs (0.313mg/L and 1.25mg/L) against Pseudomonas aeruginosa and Staphylococcus aureus, respectively. Both extracts recorded the highest MBIC (50.00%) against Pseudomonas aeruginosa. The peak MBEC (57.88%) was obtained from the methanol extract against Staphylococcus aureus and this same extract inhibited 56.23% density of bacterial biofilms on glass slides.The methanol and ethyl acetate crude extracts of R. officinalis appreciably reduced bacterial biofilms; hence, this plant can be exploited as a natural antifouling agent, with reduced toxicity and low risk of resistance.

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Section: Resultssupporting

confidence: 91%

Rosmarinus officinalis Might be Exploited as a Natural Antifouling Agent: A Potentially Promising Strategy for Curbing Membrane Biofouling

Salisu

Umar

Kawo

et al. 2022

JSPAE

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“…Zuberi* and Nadeem [42] reported in their study when compared this method in compare with other results the CRA plate method for detecting biofilm production, reported that only four isolates produced black crystalline morphology, while 111 showed orange colonies…”

Section: Congo Red Results For S Enterica Serovar Typhi Figure (2)mentioning

confidence: 91%

Comparative study of antibiofilm activity of lime juice and lithium dioxide nanoparticles against Salmonella isolated from local cheese

Khalaf¹

2018

JoBRC

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Thirty specimens of fresh white cheeses, presented for sale in different markets at different cities of Iraq were analyzed microbiologically in this study. Isolates of Salmonella, included in the specimens collected from November 2017 to January 2018, were investigated. Capacity for biofilm producing was demonstrated by two method, Tissue culture plate method (TCP) and Congo red agar (CRA).After that the antibiofilm activity of lime extract and LiO2NPs was studied as each one of them alone and then the synergistic effect was done by TCP method. The results showed that all Salmonella isolates produce biofilm but in different degrees. The results also displayed that Lime extract and LiO2NPs had antibiofilm effect against Salmonella when used alone and when the combination done between each one of these materials. In conclusion, it was observed that the specimens of fresh white cheese included in this study contained microbial contamination at a health-threatening level but we can eliminate this contamination by using lime extract and LiO2NPs.

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“…The biofilm forming ability of these bacterial isolates is studied by CRA method and tube method. Best results were observed by TM [10].…”

Section: Discussionmentioning

confidence: 93%

“…A total of 298 CLs samples were collected with sterile cotton swab moistened with sterile normal saline solution [10], then delivered with proper transport media to the medical microbiology department at Faculty of Medicine and Health Science, University of Science and Technology and processed.…”

Section: Samples Collectionmentioning

confidence: 99%

Assessment of Antibiotics Resistance and Biofilm Production Among Bacterial Species Isolated From Contact Lenses

Bin-Hameed,

Bin-Beishr,

Shames

et al. 2023

Electron. J. Univ. Aden Basic Appl. Sci.

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Contact lenses (CLs) wearing has been increased globally during recent decades, which is one of the main risk factors for developing several ocular infections. Resistant CLs bacterial infections are mainly due to the CLs contamination by bacteria producing biofilm. This study was aimed to assessment of antibiotics resistance and biofilm production among bacterial species isolated from contact lenses in Mukalla city, Hadhramout, Yemen. This cross-sectional study was carried out on 298 participants women during a period from October 2022 to January 2023. The CLs swab samples were collected, then inoculated onto culture media and incubated aerobically at 37°C for 24 hrs. The bacterial isolates were identified by conventional bacteriological methods of cultural characteristics, Gram staining and biochemical test. Antibiotics susceptibility testing was performed by disc diffusion method. Bacterial biofilm production on CLs was detected by tube method (TM) and Congo red agar (CRA) method. The prevalence of CLs bacterial infection was 54.4%. Enterobacter spp. 37.1%, followed by Escherichia coli 28.4%, Pseudomonas aeruginosa 11.7%, Klebsiella pneumoniae 6.8% were the most common Gram-negative isolated from CLs. Staphylococcus epidermides 3.7% and other coagulase negative staphylococci (CoNS) 12.3% were the most common species of Gram-positive bacteria isolated from CLs. The CRA method was found to be effective phenotypic screening method for detection of biofilm production of bacterial isolates from CLs. Prevalence of antibiotics resistance and multi-drug resistance (MDR) biofilm producing strains was found. In conclusion, there is a high prevalence of CLs use by females in Mukalla city, Hadhramout especially for cosmetic purposes. CLs infection due to improper care practices leads to eye complications. Increasing awareness is crucial to avoid identified risk factors for ocular infection.

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Detection of Biofilm Forming Ability of Bacterial Isolates from Contact Lenses and their Accessories

Cited by 3 publications

References 9 publications

Rosmarinus officinalis Might be Exploited as a Natural Antifouling Agent: A Potentially Promising Strategy for Curbing Membrane Biofouling

Rosmarinus officinalis Might be Exploited as a Natural Antifouling Agent: A Potentially Promising Strategy for Curbing Membrane Biofouling

Comparative study of antibiofilm activity of lime juice and lithium dioxide nanoparticles against Salmonella isolated from local cheese

Assessment of Antibiotics Resistance and Biofilm Production Among Bacterial Species Isolated From Contact Lenses

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