In modern era the usage of contact lens is increased in youngsters especially in females with a high risk of ocular infections. In current research bacteriological analysis and bio-film forming ability of 22 contact lens accessories samples (Contact lenses, contact lens storage kit and contact lens solution) is studied. The isolated bacteria includes Corynebacterium spp, S. aureus, coagulase negative Staphylococcus, Bacillus spp. and Streptococcus spp. Bio-film are the main cause of bacterial infection. The bio-film forming ability of these bacterial isolates is studied by Congo red agar method and tube method. Best results were observed in Test tube method. The results initiate to continue further researches on the pathogenesis of bacteria as well as guide patients with proper contact lens procedures will hopefully decrease the incidence of ocular infections. Modern approaches for bio-film prevention and control will help in elimination of different diseases. The main objective of the research is to study the contact lens accessories isolated pathogens and their bio-film forming ability.
Dromedary camel's milk is a natural source of probiotics; enzymes and secondary metabolites that have the ability to combat many pathogens. The aim of this study was to compare the effectiveness of filtered and non-filtered dromedary camel's milk activity against various pathogens. Filtered and nonfiltered (raw and boiled) dromedary milk was assessed against different pathogens by using agar well diffusion on Muller Hinton agar (MHA) and Blood agar assays. The sensitivity pattern against all pathogens was determined on MHA plate, by incubating for 24 h at 37°C. Streptococcus and Lactobacillus have antagonistic activity against various pathogens. The results showed that effectiveness of non-filtered milk was about 40 and 60% of boiled and raw milk respectively. Filtered milk had a 50% of effectiveness for both raw and boil milk. The antibacterial activity of filtered milk indicates the presence of such Immunoglobulins and enzymes that help in providing immunity. The streptococci inhibit 64% of the test organisms, while Lactobacillus suppresses 54% of pathogens. Acinetobacter baumannii is more susceptible to 37mm zone while Lactobacillus suppressed the growth of Micrococcus luteus with 45 mm zone.
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