Axial-beam absorbance detection for capillary electrophoresis

Taylor, John A.; Yeung, Edward S.

doi:10.1016/s0021-9673(01)88586-1

Cited by 40 publications

(15 citation statements)

References 14 publications

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“…As noted above, since the longer light path can be taken along the capillary, higher sensitivity is expectable for axial absorption measurements than for usual crossbeam detection. Axial absorption detection was first attempted by Yeung and coworkers [36][37][38] for capillary chromatography and then for CE. They used two different capillaries to accomplish their purpose: (i) partial inner reflection with a silica capillary and (ii) total reflection with a PTFE capillary.…”

Section: Axial Absorption Detection In Cementioning

confidence: 99%

Liquid‐core waveguide in CE

Okada

2007

Electrophoresis

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Liquid-core waveguide (LCW) brings about several advantages in CE. This review discusses some aspects of fundamental and practical importance involved in this method. Sensitivity in absorption and fluorescence detection is in general improved by more than one order of magnitude over usual crossbeam detection arrangements; the improvements come from the long light path in absorption detection and low light scattering in fluorescence detection. Versatile instrumental arrangements are another advantage of LCW in CE, leading to several detection schemes, some of which provide information that is not gained by usual capillary-end crossbeam detection, e.g. whole-capillary imaging, simultaneous monitoring of multicapillary separation, and kinetic evaluation. The high potential and perspectives of LCW in CE are discussed based on the state-of-the-art developments.

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Section: Axial Absorption Detection In Cementioning

confidence: 99%

Liquid‐core waveguide in CE

Okada

2007

Electrophoresis

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“…Poly(tetrafluoroethylene) (PTFE) has been the polymer used most frequently [13][14][15][16][17][18][19][20][21][22] and it seems that its use as large diameter capillaries (∼200 m) in the early papers may have originated from the previous use of about 200 m i.d. PTFE capillaries in capillary isotachophoresis (cITP) [23].…”

Section: Introductionmentioning

confidence: 99%

“…Several polymers, such as PTFE, are transparent at short-wavelength-UV, even though impurities and crystallinity may cause scattering of UV light [32]. It should be also noted that the unusually low refractive index of PTFE makes it a unique material for construction of totally reflecting photometric detection cells, or liquid core waveguides [18,33]. Polymer capillaries are typically also very good electrical insulators and are inert and thermally stable [34].…”

Section: Introductionmentioning

confidence: 99%

Poly(tetrafluoroethylene) separation capillaries for capillary electrophoresis

Macka

Yang

Zakaria

et al. 2004

Journal of Chromatography A

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“…Techniques based on extending the optical path length such as Z-shaped and bubble cells have been developed for the analysis of small analytes, with several tens-fold improvements in sensitivity [18,19,20,21,22,23,24,25,26,27]. Although it is easy to make Z-shaped cells, loss of resolution is problematic when analytes migrate too closely.…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoretic separation of dsDNA under nonuniform electric fields

Lin

Huang

2003

Anal Bioanal Chem

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Improved sensitivity for the analysis of DNA by capillary electrophoresis has been achieved, based on simultaneous increases in optical path length and injection volume. To increase the optical path length, bubble cells with diameters ranging from 150 to 450 microm have been fabricated and tested. In terms of resolution and sensitivity, a bubble cell of 300 microm diameter is appropriate when using 75-microm capillaries. To allow greater injection volumes, we performed on-line concentration of DNA in the presence of electroosmotic flow (EOF) using 2.0% poly(ethylene oxide) (PEO). With a 300-microm bubble cell, a 170-fold improvement in the sensitivity for the 89-bp fragment has been accomplished when injecting about 0.33 microL DNA. In the presence of the bubble cell, the resolution for the large fragments improves while that for the small ones (<124 base pair) decreases. The effect of bubble cells was further investigated by conducting DNA separation in the absence of EOF, showing that improvements in resolution are mainly due to increased migration differences when DNA migrated at low electric field strengths in the bubble region. We have suggested that such an effect is more profound using shorter capillaries, leading to complete separation of phiX 174 RF DNA-Hae III digest in 2 min.

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Axial-beam absorbance detection for capillary electrophoresis

Cited by 40 publications

References 14 publications

Liquid‐core waveguide in CE

Liquid‐core waveguide in CE

Poly(tetrafluoroethylene) separation capillaries for capillary electrophoresis

Capillary electrophoretic separation of dsDNA under nonuniform electric fields

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