Poly(tetrafluoroethylene) separation capillaries for capillary electrophoresis

Macka, Mirek; Yang, Wen-Chu; Zakaria, Philip; Shitangkoon, Aroonsiri; Hilder, Emily F.; Andersson, P.; Nesterenko, Pavel N.; Haddad, Paul R.

doi:10.1016/j.chroma.2004.03.074

Cited by 22 publications

(24 citation statements)

References 40 publications

(48 reference statements)

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“…Finally, in recent work, Macka et al [171] tested PTFE capillaries as an interesting alternative to the typical fused-silica capillaries for CE. This type of capillary provides major advantages such as excellent electrical properties, transparency in the UV region, and reasonable thermal conductivity.…”

Section: Using Coated Capillariesmentioning

confidence: 97%

MEKC: An update focusing on practical aspects

Silva

2007

Electrophoresis

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This paper reviews recent methodological and instrumental advances in MEKC. Improvements in sensitivity arising from the use of on-line sample concentration (sweeping, stacking, and combination of both protocols) and derivatization (in-capillary reactions and coupling with flow-injection systems) and improvements in resolution obtained by changing the composition of the BGE (e.g., with organic modifiers, ionic liquids, nonionic and zwitterionic surfactants, mixed micelles, and vesicles) or using coated capillaries are discussed in detail. In addition, MS and LIF spectroscopy are examined in relation to their advantages and restrictions as applied to MEKC analysis. Some thoughts on potential future directions are also expressed.

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Section: Using Coated Capillariesmentioning

confidence: 97%

MEKC: An update focusing on practical aspects

Silva

2007

Electrophoresis

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“…Fujimoto et al [20] reported the irreversible adsorption of thiourea in PEEK columns. On the other hand, the electroosmosis in nonionizable unmodified plastic columns has been related with the absorption of buffer ions on the capillary surface or the presence of additives and trace components in the column material [18][19][20][22][23][24][25][26]. In order to evaluate the stability of PEEK columns at pH 11, repeatability and reproducibility were studied.…”

Section: Peek Capillarymentioning

confidence: 99%

CE‐ESI‐MS of biological anions in plastic capillaries at high pH

et al. 2007

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In this study, the potential of poly(methylmethacrylate) (PMMA, Plexiglas) and polyether ether ketone (PEEK) tubing for CE-ESI-MS separations of anions at high pH values was examined. A set of model compounds of biological interest was used to investigate the main operational parameters for CE-ESI-MS, such as the sheath-flow interface design, the polarity of the ionization voltage, the use of ammonia-based separation electrolytes, and the sheath liquid composition. Optimum separations and detection sensitivities in negative ESI mode were obtained using a running electrolyte of 75 mM of ammonia at pH 11 and a sheath liquid of 60:40 v/v or 75:25 v/v isopropanol/water with 0.5% v/v of ammonia. At these experimental conditions, PMMA and PEEK capillaries show good hydrolytic stabilities and lower EOF values than fused-silica columns. Better separation resolutions were obtained with PMMA capillary, but this plastic rapidly swelled and bled because of its limited chemical resistance to the sheath liquid. PMMA columns equipped with a fused-silica tip were used for a safer exposure to the sheath liquid, but the inner surface of the fused-silica tips had limited stability at pH 11. On the other hand, good separations and reproducibility on migration times and peak areas were obtained using PEEK capillaries without capillary column deterioration.

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“…The capillary material should be an excellent electrical insulator to withstand high electrical field strength, possess a high thermal conductivity to allow effective heat dissipation, have suitable mechanical properties (flexible but not too soft), and be optically transparent in a wide spectral range and extending to the low-UV region. The material should also be chemically inert so that it will not react with electrolyte components or analytes, it should have a well-defined surface allowing reproducible EOF, and should have well-characterised surface chemistry to aid practical control of the EOF [1]. Since the advent of polymer-coated fused-silica (FS) technology for capillary GC [2,3], this material has been used almost exclusively as the material of choice for the separation capillary in CE.…”

Section: Introductionmentioning

confidence: 99%

“…In principle there can be other suitable capillary materials, especially synthetic polymers, and the potential of polymeric capillaries has stimulated the interest of this [1,4,5] and other groups [6][7][8][9][10][11][12][13][14][15][16]. Polymer capillaries have the advantages that they can be produced by extrusion at relatively small cost and no additional protective coating is required as is the case for FS.…”

Section: Introductionmentioning

confidence: 99%

Fluorinated ethylenepropylene copolymer as a potential capillary material in CE

et al. 2007

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In this work, a new generation UV-transparent polymer, fluorinated ethylenepropylene copolymer (FEP) exhibiting a low degree of crystallinity, extruded in dimensions similar to the most commonly used CE capillaries of approximately 80 mum id and about 360 mum od was investigated for its use as a CE capillary. FEP is transparent down to the low-UV region, and as fluorinated polymers in general are good electrical insulators and exhibit reasonable heat conductivity, it has considerable potential as a material for electrodriven analysis in capillary or microchip formats. The FEP capillary has been characterised with regard to some important aspects for its use as a CE capillary, including its profile of EOF versus pH, as well as procedures for manipulating EOF by coating the inner capillary wall with various semipermanent and dynamic layers. The FEP capillaries were tested and compared with fused-silica capillary for the separation of inorganic and small organic ions using conditions involving direct and indirect detection in the low-UV region. Finally, advantages of the use of the FEP capillary for simultaneous detection of a mixture containing nine inorganic cations and anions using indirect photometric detection with a movable light-emitting diode (LED) detector and a novel electrolyte are demonstrated.

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Poly(tetrafluoroethylene) separation capillaries for capillary electrophoresis

Cited by 22 publications

References 40 publications

MEKC: An update focusing on practical aspects

MEKC: An update focusing on practical aspects

CE‐ESI‐MS of biological anions in plastic capillaries at high pH

Fluorinated ethylenepropylene copolymer as a potential capillary material in CE

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