Association studies on the porcine RETN, UCP1, UCP3 and ADRB3 genes polymorphism with fatness traits

Cieślak, J.; Nowacka‐Woszuk, Joanna; Bartz, M.; Fijak-Nowak, Honorata; Grzes, Maria; Szydłowski, M.; Świtoński, M.

doi:10.1016/j.meatsci.2009.07.001

Cited by 16 publications

(15 citation statements)

References 24 publications

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“…The second one (c.448A > G, relative to the start codon) located in exon 3 was also a missense substitution (Arg150Gly). The two SNPs found in our study were earlier discovered by Cieslak et al (2009) and Li et al (2005), respectively.…”

Section: Detection Of Single Nucleotide Polymorphismssupporting

confidence: 82%

“…Although Cieslak et al (2009) found a significant association between UCP3 gene variants and abdominal fat weight in a mixed group (Polish Landrace and Polish synthetic line), no significant association was observed in a separate set of Polish Landrace group and Polish synthetic line. On the other hand, in our study, the UCP3 gene was associated with intramuscular fat content, but no association was observed with subcutaneous fat deposition.…”

Section: Discussionmentioning

confidence: 57%

“…Earlier experiments approved that UCP3 gene influenced muscle growth and fat convertion (Fang et al, 2002). Cieslak et al (2009) analyzed the association between T221C polymorphism and fatness trait in a mixed group (Polish Landrace and Polish synthetic line), and a significant correlation with abdominal fat weight was observed. In this current study, although the T221C and A448G variants were earlier reported by Cieslak et al (2009) andLi et al (2005), respectively, unique T221C genotype distribution and novel correlations with IMF trait were observed.…”

Section: Discussionmentioning

confidence: 99%

“…Cieslak et al (2009) analyzed the association between T221C polymorphism and fatness trait in a mixed group (Polish Landrace and Polish synthetic line), and a significant correlation with abdominal fat weight was observed. In this current study, although the T221C and A448G variants were earlier reported by Cieslak et al (2009) andLi et al (2005), respectively, unique T221C genotype distribution and novel correlations with IMF trait were observed. Botstein and Risch (2003) concluded that the majority of identified disease-associated variants were regulatory or missense polymorphisms.…”

Section: Discussionmentioning

confidence: 99%

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Association of porcine UCP3 gene polymorphisms with fatness traits in a PietrainJinhua F2 population

Chen¹,

Zhao²,

Zhu³

et al. 2011

Afr. J. Biotechnol.

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Uncoupling protein 3 (UCP3) is a mitochondrial transmembrane carrier which uncouples oxidative phosphorylation and plays an important role in energy homeostasis and fatty acid transporting. In this study, two missense mutations (T221C and A448G) were detected in porcine UCP3 gene. Genotype frequencies were analyzed using polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) in eight pig breeds (n = 384). The results revealed that Chinese native breeds had fixed genotype CC at T221C polymorphic loci. Allele G at the A448G loci has higher frequency than allele A in all pure breeds, except for Pietrain. Association analysis between variants and fatness traits was carried out in a Pietrain×Jinhua F2 population (n = 274). The results showed that two single nucleotide polymorphism (SNPs) were significantly associated with intramuscular fat content (p = 0.023 and p = 0.000, respectively). These results indicated that single nucleotide polymorphism of UCP3 gene is potentially associated with fatness traits in pig.

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Section: Detection Of Single Nucleotide Polymorphismssupporting

confidence: 82%

Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Association of porcine UCP3 gene polymorphisms with fatness traits in a PietrainJinhua F2 population

Chen¹,

Zhao²,

Zhu³

et al. 2011

Afr. J. Biotechnol.

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“…The saturated fatty acid (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) were calculated. Fatty acid composition contents were expressed as (Gerbens et al 1997;Knoll et al 1998;Cieslak et al 2009) were genotyped using the PCR-RFLP method. LPL gene polymorphisms (Wang et al 2009) were genotyped using the PCR-SSCP method described by Gasser et al (2006).…”

Section: Methodsmentioning

confidence: 99%

Association of the ADRB3, FABP3, LIPE, and LPL gene polymorphisms with pig intramuscular fat content and fatty acid composition

Xue¹,

Wang²,

Bang-quan³

et al. 2015

CZECH J ANIM SCI

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ABSTRACT:The aim of the present study was to investigate the associations of single nucleotide polymorphisms (SNPs) in candidate genes with fatness traits in the Longissimus dorsi muscle of pigs. The polymorphisms of genes were investigated, which included beta-3-adrenergic receptor gene (ADRB3), heart fatty acid-binding protein gene (FABP3), and hormone-sensitive lipase gene (LIPE) as well as lipoprotein lipase gene (LPL). The intramuscular fat (IMF) content and fatty acid composition contents in Longissimus dorsi muscle samples were measured. Results showed that ADRB3, LIPE, and LPL SNPs were associated with IMF content (P < 0.05). ADRB3 AG heterozygotes exhibited higher IMF content. LIPE A allele was associated with greater IMF content. LPL CT heterozygotes exhibited the lowest IMF content. ADRB3 c.1192G>A had highly significant association with the total monounsaturated fatty acid (MUFA) (P < 0.01) and the total polyunsaturated fatty acid (PUFA) (P < 0.01). LIPE c.442G>A was significantly associated with the contents of C12:0 and C14:0 (P < 0.05). LPL c.624C>T was significantly associated with the percentage of C16:1 (P < 0.05) and the percentage of total saturated fatty acid (SFA) (P < 0.05). The pigs with ADRB3 G allele had more MUFA, and the pigs with LPL T allele had less SFA, implying that the ADRB3 G and LPL T in pigs may be beneficial to human health. In conclusion, the results suggest that these genetic markers are important sources of the variations for the pork selection to obtain favourable meat with higher IMF levels and appropriate fatty acid composition.

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Genetics of fat tissue accumulation in pigs: a comparative approach

et al. 2010

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Fatness traits are important in pig production since they influence meat quality and fattening efficiency. On the other hand, excessive fat accumulation in humans has become a serious health problem due to worldwide spread of obesity. Since the pig is also considered as an animal model for numerous human diseases, including obesity and metabolic syndrome, comparative genomic studies may bring new insights into genetics of fatness/obesity. Input of genetic factors into phenotypic variability of these traits is rather high and the heritability coefficient (h(2)) of these traits oscillates around 0.5. Genome scanning revealed the presence of more than 500 QTLs for fatness in the pig genome. In addition to QTL studies, many candidate gene polymorphisms have been analyzed in terms of their associations with pig fatness, including genes encoding leptin (LEP) and its receptor (LEPR), insulin-like growth factor 2 (IGF-2), fatty acid-binding proteins (FABP3 and FABP4), melanocortin receptor type 4 (MC4R), and the FTO (fat mass and obesity-associated) gene. Among them, a confirmed effect on pig fatness was found for a well-known polymorphism of the IGF-2 gene. In humans the strongest association with predisposition to obesity was shown for polymorphism of the FTO gene, while in pigs such an association seems to be doubtful. The development of functional genomics has revealed a large number of genes whose expression is associated with fat accumulation and lipid metabolism, so far not studied extensively in terms of the association of their polymorphism with pig fatness. Recently, epigenomic mechanisms, mainly RNA interference, have been considered as a potential source of information on genetic input into the fat accumulation process. The rather limited progress in studies focused on the identification of gene polymorphism related with fatness traits shows that their genetic background is highly complex.

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Association studies on the porcine RETN, UCP1, UCP3 and ADRB3 genes polymorphism with fatness traits

Cited by 16 publications

References 24 publications

Association of porcine UCP3 gene polymorphisms with fatness traits in a PietrainJinhua F2 population

Association of porcine UCP3 gene polymorphisms with fatness traits in a PietrainJinhua F2 population

Association of the ADRB3, FABP3, LIPE, and LPL gene polymorphisms with pig intramuscular fat content and fatty acid composition

Genetics of fat tissue accumulation in pigs: a comparative approach

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