Association of the ADRB3, FABP3, LIPE, and LPL gene polymorphisms with pig intramuscular fat content and fatty acid composition

Abstract

ABSTRACT:The aim of the present study was to investigate the associations of single nucleotide polymorphisms (SNPs) in candidate genes with fatness traits in the Longissimus dorsi muscle of pigs. The polymorphisms of genes were investigated, which included beta-3-adrenergic receptor gene (ADRB3), heart fatty acid-binding protein gene (FABP3), and hormone-sensitive lipase gene (LIPE) as well as lipoprotein lipase gene (LPL). The intramuscular fat (IMF) content and fatty acid composition contents in Longissimus dorsi muscle samples were measured. Results showed that ADRB3, LIPE, and LPL SNPs were associated with IMF content (P < 0.05). ADRB3 AG heterozygotes exhibited higher IMF content. LIPE A allele was associated with greater IMF content. LPL CT heterozygotes exhibited the lowest IMF content. ADRB3 c.1192G>A had highly significant association with the total monounsaturated fatty acid (MUFA) (P < 0.01) and the total polyunsaturated fatty acid (PUFA) (P < 0.01). LIPE c.442G>A was significantly associated with the contents of C12:0 and C14:0 (P < 0.05). LPL c.624C>T was significantly associated with the percentage of C16:1 (P < 0.05) and the percentage of total saturated fatty acid (SFA) (P < 0.05). The pigs with ADRB3 G allele had more MUFA, and the pigs with LPL T allele had less SFA, implying that the ADRB3 G and LPL T in pigs may be beneficial to human health. In conclusion, the results suggest that these genetic markers are important sources of the variations for the pork selection to obtain favourable meat with higher IMF levels and appropriate fatty acid composition.

A comprehensive transcriptome analysis of skeletal muscles in two Polish pig breeds differing in fat and meat quality traits

“…The LIPE gene was previously analyzed regarding fatty acid composition in pigs. Xue et al (2015) showed that LIPE ( HSL ) c.442 G > A polymorphism affected the content of C12:0 and C14:0 fatty acids ( P <0.05). The correlation analysis carried out in the present study identified trends indicating that LIPE expression in the longissimus dorsi muscle is positively related to IMF and backfat thickness values (but after Bonferroni correction the results were not significant).…”

“…When analyzing intramuscular fat (IMF), which is considered as the main factor determining the flavour of meat, it was observed that mutations in H-FABP , ( Pang et al. , 2006 ), LPL and LIPE genes ( Xue et al , 2015 ) influenced IMF content. In turn, Zhang et al.…”

Genome-wide analysis of DNA methylation in obese, lean and miniature pig breeds

“…CAMK2D was specifically expressed in pig skeletal muscle and regulated by miR-1207-5p 71 ; it was expressed at a much lower level in Wuzhishan pigs than in Landrace and Tongcheng pigs 28 . The ADRB3 gene, a major mediator of lipolytic and thermogenic effects in adipose tissue, was reported to associate with fatness traits by several studies 72 73 74 . IGF1R was identified as a potential candidate gene\for postnatal growth and carcass composition traits in pigs 75 and plays a crucial role in skeletal muscle development and differentiation 76 .…”

Decreased testosterone levels after caponization leads to abdominal fat deposition in chickens

“…GO and KEGG analyses based on 872 known DEGs were performed, and 90 DEGs related to lipid metabolism were further screened, including RXRG , FABP3 , and FABP7 [ 26 – 29 ]. Among the nine enriched pathways, classical pathways related to lipid metabolism (PPAR and fatty acid metabolism) were screened, and six representative DEGs related to lipid metabolism ( APOA1 , SCD , FABP3 , FABP7 , RXRG, and FADS2 ) were enriched and identified in the PPAR pathway.…”