Association of TLR4 Gene Genotype and Resistance Against Salmonella enteritidis Natural Infection in Kampung Chicken

Ulupi, Niken; Muladno,; Sumantri, Cece; Wibawan, I Wayan Teguh

doi:10.3923/ijps.2013.445.450

Cited by 11 publications

(8 citation statements)

References 9 publications

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“…While, the others breeds presented three genotypes of AA, BB, and AB. Ulupi et al (2013) identified three genotypes (AA, AG and GG) in TLR4-exon 2 and recorded that resistant to salmonella enteritidis natural infection in the kampung chicken in all genotypes.…”

Section: Genotype Frequencymentioning

confidence: 99%

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Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt

Khatab¹,

Hemeda²,

El‐Nahas³

et al. 2017

AJVS

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Key words:Toll-like receptor, polymorphism, genetic resistance, salmonella enteritidis, chickenThe aim of this study is to investigate the polymorphism in exon1 and 2 of TLR4 gene and its possible association with resistance to salmonella infection in the Fayoumi breed and Hyline strain of chickens and its possible association with immune response of birds under study. Two experiments were done, in which the chicks were infected intraesophageal with two different doses of S. enteritidis LD 50 (10 8 cfu) in experiment I and 1/2 LD 50 (5× 10 7 cfu) in experiment II. The birds (100 chicks from each breed) allocated into different groups according to the clinical signs in experiment I to susceptible and resistant and in experiment II, to high and low immune response groups based on ELISA test. TLR4 gene was genotyped by PCR-RFLP of exon 1 (596 bp) and exon 2 (793 bp) using different restriction enzymes. Comparison between the different genotypes generated by Taq1 of Fayoumi breed and Hy-line strain in experiment I and II give valuable result. Exon 1-Taq1 AA genotype can be used as a marker for culling the birds of Hy-line strain as it appears in low immune response and also, BB genotype (0.7) in susceptible birds. Moreover, using exon 2-EcoR-1 BB genotype (0.9) in susceptible birds as a marker for culling these birds. We concluded that the Polymorphisms of TLR4 -exon 1 and 2 can be used as a marker-assisted selection for resistant and/or susceptibility to salmonella infection in Hy-line strain.

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Section: Genotype Frequencymentioning

confidence: 99%

“…It is the most important markers showing a resistance of chicken against salmonella (Ulupi et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt

Khatab¹,

Hemeda²,

El‐Nahas³

et al. 2017

AJVS

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“…S. enteritidis is one of the zoonotic bacteria that can be transmitted through food (food-borne disease). Ulupi et al (2013) found that 3.12% of chicken eggs were positively contaminated with S. enteritidis in Bogor. The incidence of salmonellosis in humans is caused by con-suming chicken eggs containing the bacteria S. enteritidis (Velge et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Polymorphisms and Associations of the NRAMP-1 and iNOS Genes on Newcastle Disease and Salmonella enteritidis Resistances in SenSi-1 Agrinak Chickens

Ardiyana

Gunawan

Murtini

et al. 2020

Trop. Anim. Sci. J.

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NRAMP-1 and iNOS genes were reported to be associated with a defense mechanism against bacteria and virus infections. This study aimed to identify NRAMP-1 and iNOS genes polymorphisms and their associations with the defense mechanisms against Salmonella enteritidis and Newcastle Disease (ND) in SenSi-1 Agrinak chicken. The present study used a total number of 172 SenSi-1 Agrinak chicken. Identifications of NRAMP-1 and iNOS genes polymorphisms were performed by PCR-RFLP method. NRAMP-1 and iNOS genotypes were associated with immunoglobulin Y (IgY) concentration, specific antibodies against S. enteritidis and ND using General Linear Model (GLM). Immunity characteristics were further grouped into high, medium, and low categories. NRAMP-1|SacI exon 11 and iNOS|AluI intron 24 in SenSi-1 Agrinak chickens were polymorphic. TC genotype has a higher immune response to infectious agents compared to TT and CC genotypes. The frequency of C allele was higher than the T allele in the concentration of immunoglobulin Y (IgY), antibodies titers against S enteritidis and ND. The TC genotype of NRAMP-1 gene was significantly associated with ND antibody titers, and the TT genotype of iNOS was significantly associated with S. enteritidis specific antibody. NRAMP-1 and iNOS genes can be used as potential candidate genes for immune traits in SenSi-1 Agrinak chickens.

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“…Similarly, genotype GG has been associated in Sentul chicken against salmonella and Newcastle disease (Mamutse et al, 2018). However, all genotypes (AA, AG, and GG) were also found with a similar resistance pattern against salmonella in Kampung chicken (Ulupi et al, 2013). A group finds polymorphism in TLR4 gene's exon 2 in 14 Chinese chicken including Red Jungle fowl and Tibetan chicken.…”

Section: Polymorphism In Different Tlrs In Chickenmentioning

confidence: 99%

The Potential of Toll-Like Receptors to Modulate Avian Immune System: Exploring the Effects of Genetic Variants and Phytonutrients

et al. 2021

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Toll-like receptors (TLRs) are pathogen recognition receptors, and primitive sources of innate immune response that also play key roles in the defense mechanism against infectious diseases. About 10 different TLRs have been discovered in chicken that recognize ligands and participate in TLR signaling pathways. Research findings related to TLRs revealed new approaches to understand the fundamental mechanisms of the immune system, patterns of resistance against diseases, and the role of TLR-specific pathways in nutrient metabolism in chicken. In particular, the uses of specific feed ingredients encourage molecular biologists to exploit the relationship between nutrients (including different phytochemicals) and TLRs to modulate immunity in chicken. Phytonutrients and prebiotics are noteworthy dietary components to promote immunity and the production of disease-resistant chicken. Supplementations of yeast-derived products have also been extensively studied to enhance innate immunity during the last decade. Such interventions pave the way to explore nutrigenomic approaches for healthy and profitable chicken production. Additionally, single-nucleotide polymorphisms in TLRs have shown potential association with few disease outbreaks in chickens. This review aimed to provide insights into the key roles of TLRs in the immune response and discuss the potential applications of these TLRs for genomic and nutritional interventions to improve health, and resistance against different fatal diseases in chicken.

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Association of TLR4 Gene Genotype and Resistance Against Salmonella enteritidis Natural Infection in Kampung Chicken

Cited by 11 publications

References 9 publications

Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt

Polymorphisms of TLR4 Gene and Its Association With Genetic Resistance to Salmonella Enteritidis Infection in Fayoumi Breed and Hy-line Strain in Egypt

Polymorphisms and Associations of the NRAMP-1 and iNOS Genes on Newcastle Disease and Salmonella enteritidis Resistances in SenSi-1 Agrinak Chickens

The Potential of Toll-Like Receptors to Modulate Avian Immune System: Exploring the Effects of Genetic Variants and Phytonutrients

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