Association of Mx Gene Genotype with Antiviral and Production Traits in Tolaki Chicken

Pagala, Muhammad Amrullah; Muladno,; Sumantri, Cece; Murtini, Sri

doi:10.3923/ijps.2013.735.739

Cited by 23 publications

(23 citation statements)

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“…Mx is located in chromosome 1 (GenBank accession number: DQ788615) having a fragment length of 20,767 bp and consisting of 13 exons, 1115 bp coding regions, and 705 remaining amino acids (Permatasari et al 2015). Mx has been linked to chicken immunity levels against viral diseases, such as AI and ND (Ko et al 2002;Ko et al 2004;Pagala et al 2013;Pagala et al 2017). Mx and the large GTPase protein it encodes are among the most studied interferon-stimulated antiviral effector molecules (Verhelst et al 2013;Fulton et al 2014).…”

Section: Discussionmentioning

confidence: 99%

Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung

et al. 2020

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Abstract. Afifah D, Lesmana I, Poerwanto SH, Joko T, Mahardhika IWS, Daryono BS. 2020. Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung. Biodiversitas 21: 1483-1487. The Gama Ayam Research Team has implemented marker-assisted selection (MAS) in selective breeding to provide a faster, more accurate, and more reliable selection of chicken. Mx gene expression has a vital role in chicken disease resistance. This research aimed to investigate the expression of Mx exon-13 single nucleotide polymorphisms (SNPs) in the population of female Layer Lohmann Brown-Classic, male Pelung, and its progenies Kampong-Laying Type (Kamper) chicken. The G1892A mutation in Mx exon-13 resulted in a change in the amino acid 631 of Mx. The substitution of serine to asparagine favored the ability of chickens to acquire immunity against viral diseases, including avian influenza. Asparagine (A allele) at position 631 is specific to Mx+ (resistant), whereas serine (G allele) is specific to Mx− (susceptible). DNA was amplified using the forward primer 5ʹ-GCACTGTCACCTCTTAATAGA-3ʹ and the reverse primer 5ʹ-GTATTGGTAGGCTTTGTTGA-3ʹ and then sequenced using the Sanger sequencing method. Four SNPs were obtained through Mx sequence alignment. They consisted of four substitutions (A20734T, C20737T, A20766G, and A20893G) with one haplotype. Mx exon-13 SNPs were detected in Pelung and Kamper. Therefore, Kamper chicken inherited the disease resistance gene of Pelung and could be a strong candidate for parental generation in the further selective breeding program.

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Section: Discussionmentioning

confidence: 99%

Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung

et al. 2020

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“…Ayam kampung memiliki warna bulu, bobot badan, pertumbuhan dan produksi telur yang masih beragam dan penyebarannya masih tersebar di seluruh Indonesia. Ayam kampung memiliki keunggulan yaitu tahan terhadap serangan bakteri dan virus (Ulupi et al, 2013;Ulupi et al, 2014;Pagala et al, 2013). Ayam kampung dan ayam lokal di Indonesia menyumbang sebesar 10% dengan konsumsi dagingnya sebesar 0,521 kg/tahun (BPS 2015).…”

Section: Pendahuluanunclassified

Untitled

Harahap¹,

Sumantri²,

Ulupi³

et al. 2017

JVet

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Calpastatin (CAST) gene is one of the genes that play a role in the process of tenderization of meat. CAST gene serves as an inhibitor of meat tenderness. The purpose of this study was to identify Single Nucleotide Polymorphisms (SNP) in the CAST gene of kampong chickens. A total of 61 kampong chickens used were used in the study including strain cobb, F1 of strain cobb-kampong, merawang, sentul, nunukan, and pelung, respectively. The method used is extracted DNA from blood samples, then amplified by Polymerase Chain Reaction (PCR) and then genotyping by DNA sequencing. The results showed that SNP was identified at position g.42988G> T in the intron 11 CAST gene of merawang chicken with genotype GG and GT. SNP was not identified in kampong chicken, strain cobb, F1 of strain cobb-kampong, sentul, nunukan, and pelung chicken, respectively. In conclusion, the intron 11 CAST gene region with 482 of product length of merawang chicken is polymorphic, whereas the other chicken species is monomorphic.

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“…Maeda (2005) melakukan penelitian gen Mx pada ayam lokal Indonesia dan melaporkan bahwa sebesar 63% ayam lokal Indonesia tahan terhadap infeksi virus AI sedangkan 37% yang berada pada kategori tidak tahan. Pagala et al (2013) melakukan penelitian pada ayam Tolaki dan melaporkan bahwa gen Mx|Hpy8I dapat digunakan sebagai penciri genetik untuk sifat ketahanan tubuh ayam terhadap infeksi virus Newcastle Disease (ND). Selain itu, dilaporkan pula adanya asosiasi keragaman genotipe gen Mx|Hpy8I terhadap sifat antiviral pada ayam Tolaki.…”

Section: Pendahuluanunclassified

“…Frekuensi ayam kampung yang membawa alel tahan terhadap infeksi virus pada penelitian ini lebih tinggidaripada yang dilaporkan oleh Maeda (2005) pada ayam lokal Indonesia yang menyebutkan bahwa sebesar 63% ayam lokal Indonesia tahan terhadap infeksi virus AI sedangkan 37% yang berada pada kategori tidak tahan. Hasil penelitian Pagala et al (2013) melaporkan bahwa sebesar 72% ayam Tollaki tahan terhadap infeksi virus ND, sedangkan 28% ayam Tollaki tidak tahan. Hal ini menunjukkan bahwa ayam Tollaki lebih tahan terhadap infeksi virus dibandingkan ayam kampung.…”

Section: Restriction Fragment Lenght Polymorphism (Rflp)unclassified

Keragaman Gen Myxovirus (Mx) pada Ayam Kampung Terseleksi Polymorphisms of Myxovirus Gene in Selected Kampung Chicken

Permatasari

Sumantri

Darwati

et al. 2015

JIPTHP

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The Mx gene is wellknown as marker genetic for chicken resistance of virus infection. The aim of this study was to analyze the mx gene polymorphisms in selected kampung chicken. A total of 25 heads of kampung chicken, which had been selected for ND virus resistant, were identified by PCR-RFLP method.The Mx gene used Hpy8I restriction enzyme where digested at GTN|NAC nucleotides. There were two alleles, A and G with three genotypes AA, AG dan GG. The result showed that 69.2% of kampung chicken were resistant toward the virus infection. This population was in Hardy-Weinberg equilibrium (x 2 < x 2 0.05

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Association of Mx Gene Genotype with Antiviral and Production Traits in Tolaki Chicken

Cited by 23 publications

References 5 publications

Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung

Expression of Mx exon-13 SNPs in Kampong-Laying Type (Kamper) chicken crossbreeds of female Lohmann brown-classic and male Pelung

Untitled

Keragaman Gen Myxovirus (Mx) pada Ayam Kampung Terseleksi Polymorphisms of Myxovirus Gene in Selected Kampung Chicken

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