Assessment of the Combined Effects of Acetamiprid and Propineb in Vivo

Abstract

Pesticides are among commonly used chemicals in agriculture and are one of major environmental pollutants. Acetamiprid and Propineb are widely used to control sucking insects and fungal infections on crops, respectively. The study presented aimed to research genotoxic effects of mixture of Acetamiprid and Propineb, in vivo. It was observed that mixture of Acetamiprid+Propineb increases the frequency of micronucleated polychromatic erythrocytes (MNPCE) at all concentrations for 24 and 48 h depending on concentrations. But these increases were not significant. The combined effect of the Acetamiprid and Propineb on bone marrow cells of mice in vivo was found to be antagonistic in terms of percentage of MNPCE. In addition, mixture of Acetamiprid and Propineb significantly decreased polychromatic erythrocytes/normochromatic erythrocytes (PCE/NCE) ratio at all concentrations. The results of the present investigation revealed that Acetamiprid was non-genotoxic, while mixture of the Acetamiprid and Propineb may have cytotoxic effects for mice bone marrow cells. But, additional in vivo and in vitro mutagenicity studies measuring different levels of DNA damage are still necessary.

Toxicities of Neonicotinoid‐Containing Pesticide Mixtures on Nontarget Organisms

“…Imidacloprid and metalaxyl influenced micronucleus formation and sister chromatid exchanges in polychromatic erythrocytes of rat bone marrow (Demsia et al 2007). A similar joint genotoxic toxicity was observed for an acetamiprid–propineb mixture, both in vivo in mouse bone marrow cells and in vitro in human lymphocytes (Muranli et al 2015; Rasgele 2017). This might be partly attributable to the decrease in the ratio of polychromatic erythrocytes to normochromatic erythrocytes, manifesting as an increase in the frequency of micronucleus formation.…”

“…This might be partly attributable to the decrease in the ratio of polychromatic erythrocytes to normochromatic erythrocytes, manifesting as an increase in the frequency of micronucleus formation. However, acetamiprid alone was not genotoxic (Rasgele 2017). These reports indicate that genotoxic effects of neonicotinoid and fungicide mixtures are possible, although the actual mechanism is still unknown.…”

Asetamipridin Fare Kemik İliği Hücrelerinde KA (Kromozomal Aberasyon) ve MN (Mikronükleus) Test Yöntemleri ile Genotoksik Etkisinin Araştırılması

“…Some studies have shown that ACE causes acute toxicity and also damages the nervous, reproductive and immune systems (5). It has been found to have genotoxic effects alone or in combination with other pesticides (15)(16)(17)(18). In this study, we aimed to determine whether ACE in the neonicotinoid group shows genotoxicity in mouse bone marrow cells by using CA and MN analysis system.…”

Genotoxicity of mixture of imidacloprid, imazalil and tebuconazole

“…The effects of the mixtures were explained by the concentration addition model [ 24 ]. Acetamiprid and propineb separately were genotoxic in mice bone marrow in vivo, but in combination these pesticides caused the antagonistic effect, decreasing the incidence of micronucleated PCE in comparison to separate treatments [ 25 ]. It is clear that mixtures of pesticides can induce genotoxic effects even if each of them separately does not manifest such activity in testing.…”