Asetamipridin Fare Kemik İliği Hücrelerinde KA (Kromozomal Aberasyon) ve MN (Mikronükleus) Test Yöntemleri ile Genotoksik Etkisinin Araştırılması

Abstract

In this study, we aimed to determine acetamipridine's (ACE) genotoxic effect in mus musculus var. albinos bone marrow cells by chromosomal aberration (CA) and micronucleus (MN) analysis. Mice were divided into four groups. Group 1 determined as negative control (NC), group 2, 3 and 4 were determined as 5 mg/kg ACE, 10 mg/kg ACE, and 15 mg/kg ACE groups respectively. ACE was given orally for 14 days. CA, mitotic index (MI) and MN frequencies were determined in femoral cells. As a result, no genotoxic effects were observed in groups treated with 5-10 mg/kg ACE compared to negative control. CA rates of 15 mg/kg ACE group was determined to be high (P<0.001). In addition, MI ratio in the 15 mg/kg ACE group was lower than the control group. When the ratios of the polychromatic erythrocyte with micronucleus were examined, no significant difference was found between the 5 mg/kg and 10 mg/kg ACE groups and control group. However, a statistically significant increase in MNPCE rates was observed in the 15 mg/kg ACE group compared to control ones (P<0.001). It was concluded that ACE at 15 mg/kg dose was genotoxic-cytotoxic.