Assessment of multicomponent hydrogel scaffolds of poly(acrylic acid-2-hydroxy ethyl methacrylate)/gelatin for tissue engineering applications

Abstract

The article describes the design of the multicomponent hydrogel system of poly(acrylic acid-HEMA)/gelatin for tissue engineering application. Derivative of polycaprolactone-diol (polycaprolactone diacrylate (PCL-DAr)) was used to cross-link acrylate monomers whereas gelatin was kept free for cell proliferation. Epigallocatechin gallate (EGCG), an anti-oxidant phytochemical, was loaded by diffusion method. Its in vitro release study in PBS (pH 6.5) at 37 ± 0.2°C (75 rpm) revealed a sustained release profile upto 20 days. Fitting of drug release data in Korsmeyer-Peppas model equation revealed probable release mechanism through the value of release coefficient (n), which was found to depend on formulations composition. Drug-polymer interaction, thermal behavior, and surface morphology were investigated by attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy, thermogravimetric analysis (TGA), and scanning electron microscopic (SEM). Swelling behavior of hydrogel in PBS (pH 6.5 and 7.4, 0.2 M) and in distilled water was found to increase with increasing AAc/HEMA ratio. Compression modulus decreased from 203 ± 3.7 KPa to 11.6 ± 1.1KPa, at 30% strain, whereas displacement values significantly increased from 3.2 ± 0.2 to 4.7 ± 0.6 mm at 20 N force (p < 0.05), with increasing AAc/HEMA ratio. Percentage cell viability was analyzed using indirect 3-[4, 5-dimethylthiazolyl-2]-2,5-diphenyltetrazo-liumbromide (MTT) assay with fibroblast L929 cells; showed ≥92.3% cell viability after 24 h incubation. Cell proliferation on the scaffold surface was found to increase with incorporation of HEMA in P(AAc)/G cross-linked hydrogel matrix upto a certain extent. These biocompatible, elastic, and swellable hydrogels can serve as a matrix for drug delivery and tissue engineering applications.

d-Glucose based bisacrylamide crosslinker: synthesis and study of homogeneous biocompatible glycopolymeric hydrogels

“…22 In this assay too both the cell lines grew in a comparable manner to the untreated sample at all the concentrations tested (Fig. At the end of 5 days all the samples attained conuence and the surface of the well was completely covered with the growing cells.…”

PVA-based hydrogels for tissue engineering: A review

“…This polymer becomes polyelectrolyte in water through dissociation of the acid groups [219]. PAAc has been used as hydrogel material in different tissue engineering applications [220][221][222]. weeks period [223].…”

Influence of Poly(acrylic acid) on the Mechanical Properties of Composite Hydrogels

“…It is, hence, possible to make a composite hydrogel, which is desirable for tissue regeneration by promoting cell adhesion, migration, differentiation, and proliferation. 9 Gel is also of potential interest because of its ability to be cross-linked with chitosan, 10 poly(vinyl) alcohol, [11][12][13][14] poly(ethylene glycol), 15 sodium alginate, 16 and poly(acrylic acid) (PAA). 7 However, due to the lack of mechanical strength, it cannot be used to support the growth of the new tissue.…”