The ability of sugar pendants in glycopolymers to mimic that on the cell surface can be used as a reliable method for the site specific delivery of drugs. In the search for new possibilities, we herein report a Dglucose based synthesis of hitherto unknown bisacrylamide crosslinker (Glc-bis), a related acrylamide monomer (Glc-acryl), with hemiacetal functionality. A one pot process to transform the synthesized Glcacrylamides to sterilized homogeneous glycopolymeric gel (Glc-gel) was investigated using radiation induced polymerization technique. The Glc-gel was subjected to swelling studies, viscoelastic, and thermal analysis. The in vitro cytotoxicity test revealed that the constituents (Glc-acryl and Glc-bis) as well as the Glc-gel were non toxic to the cells and the cells grew normally in their presence. The synthesized Glc-gel was found to show good affinity to lectin Con A demonstrating that replacing hydroxyl group in D-glucose moiety with amide bond does not largely affect its interaction with lectins.
A novel synthetic method was developed to synthesize resorcinol-formaldehyde (RF) resin in spherical form, of required mesh size, using XAD-4 as template beads. The synthesized RF-coated XAD (RF-XAD) beads were characterized, using different techniques. Suitable size and mechanical stability, along with their spherical shape, make these beads most appropriate for column operation. The efficiency of these beads was evaluated for removal of cesium from alkaline medium, in batch conditions, using a radioanalytical technique. The effect of sodium ion concentration, the initial cesium ion concentration and the contact time were also investigated. It was observed that the K d value for Cs + ions decreases with increase in Na + ion concentration. The equilibrium data were fitted into different isotherm models, and were found to be represented well by the Langmuir isotherm equation, with a monolayer sorption capacity of 287 mg g 21 . Kinetic modeling analysis, using pseudo first-order, pseudo second-order and intraparticle diffusion equations, shows that the pseudo secondorder equation is the most appropriate model for the description of the sorption of cesium ions onto the RF-XAD beads. The rate constants were determined at different initial concentrations. The process mechanism was found to be complex, consisting of both surface sorption and pore diffusion.
Gold nanoparticles (AuNPs) with shelf lives of more than 6 months at 25 °C were synthesized by in situ reduction and stabilization using a D-glucose-based bis(acrylamide) solution. Transmission electron microscopy (TEM) images support the homogeneous coating of AuNPs by Glc-bis. In the process, intrinsic emission of self-assembled glycobis(acrylamide) (Glc-bis) was quenched by AuNPs, and the resultant system, Glc-bis@AuNPs, can work as a turn-on sensor, wherein the quenched emission was restored upon the addition of lectin, Concanavalin A (Con A). The aggregation of Glc-bis@AuNPs upon interaction with Con A was pragmatic from the TEM images. The quenching as well as recovery of the emission based on the proximity of AuNPs with Glc-bis in Glc-bis@AuNPs was further confirmed by steady-state as well as fluorescence lifetime measurements. The Glc-bis@AuNPs system was found to be stable up to 1 M NaCl concentration, a factor that fulfills the requirement for biosensing applications. The binding constant of Glc-bis@AuNPs toward Con A was 9.8 × 10 4 M −1 , while the limit of detection was 1.6 nM. The interaction of Glc-bis@ AuNPs with a FimH-positive, pathogenic K12 strain of Escherichia coli resulted in restoration of the emission, but experiments with the FimH-negative TOP10 strain did not yield any emission.
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