Gelatin-lecithin-F127 gel mediated self-assembly of curcumin vesicles for enhanced wound healing

3,3′-Diselenodipropionic acid immobilised gelatin gel: a biomimic catalytic nitric oxide generating material for topical wound healing application

“…48 Having confirmed the NO generating activity of gelatin–DSePA conjugate, it (5% w/v in water) was mixed with additives such as lecithin (6% w/w of gelatin) and pluronic F127 (3% w/w of gelatin) and processed to prepare G–Se–Se–G gel as per methods reported previously from our group. 31 The additives such as lecithin and F127 were used to improve the mechanical strength of the gel as gelatin per se has the limitation of poor mechanical strength. 49 Indeed, rheological characterisation of G–Se–Se–G gel suggested that it exhibited the desirable mechanical and thermal stability required for topical application.…”

“…The gel (henceforth mentioned as G–Se–Se–G gel) was prepared by temperature dependent gelation method, as reported previously by our group. 31 In brief, DSePA conjugated gelatin (5% w/v) was mixed with lecithin (6% w/w of gelatin) and pluronic F127 (3% w/w of gelatin) in 2 ml of nanopure water, heated at 65 °C with continuous stirring up to 30 min and then allowed to cool at room temperature forming the gel. The morphological characterization of the gel was performed by scanning electron microscope (SEM).…”

“…The fine tissue sections of 5 µm thickness were cut from paraffin embedded wound tissue and subjected to Haematoxylin and Eosin (H&E) and Masson's Trichome (MT) staining, as mentioned previously. 31…”

“…The content of hydroxyproline and lipid peroxidation in the tissue extract was estimated using the colorimetric (560 nm and 532 nm, respectively) method as reported in previous publications. 31,39,40…”

“…30 Our group has recently shown that gelatin gel of optimum mechanical strength can be prepared by subjecting the aqueous mixture containing gelatin (5% w/v), lecithin (6% w/w of gelatin), and F127 (3% w/w of gelatin) to temperature dependent gelation. 31 Further, with regard to organodiselenide, our group has previously reported the GPx like activity of DSePA using acellular and cellular models. 32 Additionally, this compound is also reported for various other pharmacological applications such as radioprotection, anticancer, anti-haemolytic, heavy metal sequestration, and NO generating catalyst, among others.…”