Assessment of Genotypic Variation among Cultivated Durum Wheat Based on EST-SSRs and Genomic SSRs

Eujayl, Imad A.; Sorrells, Mark E.; Baum, Michaël; Wolters, Petra; Powell, W.

doi:10.1007/978-94-017-3674-9_80

Cited by 84 publications

(76 citation statements)

References 6 publications

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“…Although the exclusive alleles may only be inferred for the sampled gene pool, the results presented here may be useful for differentiating accessions and fingerprinting varieties of our coffee breeding program, as suggested in other species (Donini et al, 1998, Eujayl et al, 2001, He et al, 2005. Null alleles were observed here with gSSR primers, but not with EST-SSR primers.…”

Section: Discussionsupporting

confidence: 47%

“…Higher percentages of polymorphism with gSSR markers were also reported for grape (Scott et al, 2000), rice (Cho et al, 2000), wheat (Eujayl et al, 2001), sugar cane (Cordeiro et al, 2001), and barley (Chabane et al, 2006). It may occur due to a high conservation of DNA sequences in transcribed regions of the genome (Varshney et al, 2005), such as the ESTs.…”

Section: Discussionmentioning

confidence: 90%

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Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers

Missio

Caixeta²,

Zambolim³

et al. 2011

Genet. Mol. Res.

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Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 90%

Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers

Missio

Caixeta²,

Zambolim³

et al. 2011

Genet. Mol. Res.

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“…Express Sequence Tags derived from SSRs (ESTSSRs), as genetic markers, have been evaluated in several studies and these tend to be considerably less polymorphic than those from genomic DNA for wheat (Eujayl et al 2001). However, EST-SSRs have received a lot of attention because they are derived from the transcribed region of genes responsible for the traits of interest.…”

mentioning

confidence: 99%

An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat

Hasançebi¹,

Mert²,

Ertuğrul³

et al. 2014

CZECH J GENET PLANT

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Hasancebi S., Mert Z., Ertugrul F., Akan K., Aydin Y., Senturk Akfirat F., Altinkut Uncuoglu A. (2014): An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat. Czech J. Genet. Plant Breed., 50: 11-18.EST-SSR markers, derived from the A and B genomes of wheat were used to identify molecular markers associated with yellow rust resistance. For this purpose, bulk segregant analysis was performed using 114 EST-SSR primer pairs. They were screened on the parent genotypes and resistant/susceptible DNA pools from the cross between Izgi2001 (resistant male parent) × ES14 (susceptible female parent) at the seedling and adult plant stage. An EST-SSR marker, bu099658, generated the 206 bp DNA fragment that was present in the resistant parent and resistant bulk, but it was not present in the susceptible parent and the susceptible bulk. To investigate its association with Yr genes, 20 individuals of NILs were also amplified with BU099658 and the 206 bp marker fragment was obtained only in Yr1/6 × Avocet S. Additionally, bu099658 was screened on 65 genotypes which possessed different Yr genes/gene combination(s) and Yr1. The results indicate a close linkage of bu099658 with the Yr1 gene.

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“…The goal was to obtain useful molecular data regarding the local gene pool. Wheat genotypes have been analyzed using different DNA marker systems and have identified genetic diversity or similarity levels within a specific group of genotypes (Eujay et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat

Ejaz¹,

Zhu²,

Zhang³

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Amplified fragment length polymorphism markers were used to assess genetic diversity in 10 male sterile wheat crop lines (hetero-cytoplasm with the same nucleus) in relation to a restorer wheat line. These male sterile lines were evaluated using 64 amplified fragment length polymorphism primer combinations, and 13 primers produced polymorphic bands, generating a total 682 fragments. Of the 682 fragments, 113 were polymorphic. The polymorphic information content and marker index values demonstrated the utility of the primer combinations used in the present study. Unweighted pair group method with arithmetic mean and principal coordinate analysis of the genotypic data revealed clustering of accessions based on genetic relationships, and accessions were separated into 2 groups with their restorer line. M. Ejaz et al. 8936©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8935-8946 (2015) Jaccard's similarity coefficient values suggested good variability among the male sterile lines, indicating their utility in breeding programs. The fallouts of analysis of molecular variance showed large within-group population variation, accounting for 77% of variation, while amonggroup comparison accounted for 23% of the total molecular variation, which was statistically significant. The molecular diversity observed in this study will be useful for selecting appropriate accessions for plant improvement and hybridization through molecular-breeding approaches and for developing suitable conservation strategies.

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Assessment of Genotypic Variation among Cultivated Durum Wheat Based on EST-SSRs and Genomic SSRs

Cited by 84 publications

References 6 publications

Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers

Genetic characterization of an elite coffee germplasm assessed by gSSR and EST-SSR markers

An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat

Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat

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