An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat

Abstract

Hasancebi S., Mert Z., Ertugrul F., Akan K., Aydin Y., Senturk Akfirat F., Altinkut Uncuoglu A. (2014): An EST-SSR marker, bu099658, and its potential use in breeding for yellow rust resistance in wheat. Czech J. Genet. Plant Breed., 50: 11-18.EST-SSR markers, derived from the A and B genomes of wheat were used to identify molecular markers associated with yellow rust resistance. For this purpose, bulk segregant analysis was performed using 114 EST-SSR primer pairs. They were screened on the parent genotypes and resistant/susceptible DNA pools from the cross between Izgi2001 (resistant male parent) × ES14 (susceptible female parent) at the seedling and adult plant stage. An EST-SSR marker, bu099658, generated the 206 bp DNA fragment that was present in the resistant parent and resistant bulk, but it was not present in the susceptible parent and the susceptible bulk. To investigate its association with Yr genes, 20 individuals of NILs were also amplified with BU099658 and the 206 bp marker fragment was obtained only in Yr1/6 × Avocet S. Additionally, bu099658 was screened on 65 genotypes which possessed different Yr genes/gene combination(s) and Yr1. The results indicate a close linkage of bu099658 with the Yr1 gene.

Haplotype analysis of Ethiopian bread wheat (Triticum aestivum) cultivars and elite lines for yellow rust resistance genes using linked molecular markers

“…An EST-SSR marker, BU099658 and STM marker, Stm673acag (1.1 cM linkage with Yr1 gene) were used to identify this gene in 74 wheat genotypes. The expected fragment size for BU099658 marker was 206 bp (Hasancebi et al, 2014). In the present study, the marker amplified DNA fragment sizes of 157 to 206 bp.…”

“…Bansal et al (2009) reported a molecular marker, stm673acag, that amplified 120 and 124 bp products in most lines carrying Yr1 gene, but in the present study the marker amplified a 129 bp fragment size, which may be due to technical reasons. Hasancebi et al (2014) reported the second marker, bu099658 that is linked to Yr1 gene. This marker amplified 206 bp only in those lines that possessed Yr1 genes, including Yr1/6*Avocet S. However, this marker amplified 162 bp product on the positive control (Yr1*Avocet S).…”

Identification of Stripe Rust Resistance in Ethiopian Durum Wheat by Phenotypic Screening and Kompetitive Allele Specific PCR (KASP) SNP Markers

“…Yr1, is mapped on chromosome 2AL. SSR markers have been used for haplotyping of this gene [38,41,61] in many wheat materials and various levels of detection frequencies were reported. With the KASP SNP marker IWB81533, no detection was achieved in the present study.…”