Analysis of genetic diversity identified by amplified fragment length polymorphism marker in hybrid wheat

Abstract

ABSTRACT. Amplified fragment length polymorphism markers were used to assess genetic diversity in 10 male sterile wheat crop lines (hetero-cytoplasm with the same nucleus) in relation to a restorer wheat line. These male sterile lines were evaluated using 64 amplified fragment length polymorphism primer combinations, and 13 primers produced polymorphic bands, generating a total 682 fragments. Of the 682 fragments, 113 were polymorphic. The polymorphic information content and marker index values demonstrated the utility of the primer combinations used in the present study. Unweighted pair group method with arithmetic mean and principal coordinate analysis of the genotypic data revealed clustering of accessions based on genetic relationships, and accessions were separated into 2 groups with their restorer line. M. Ejaz et al. 8936©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (3): 8935-8946 (2015) Jaccard's similarity coefficient values suggested good variability among the male sterile lines, indicating their utility in breeding programs. The fallouts of analysis of molecular variance showed large within-group population variation, accounting for 77% of variation, while amonggroup comparison accounted for 23% of the total molecular variation, which was statistically significant. The molecular diversity observed in this study will be useful for selecting appropriate accessions for plant improvement and hybridization through molecular-breeding approaches and for developing suitable conservation strategies.

Morphological and sequence-related amplified polymorphism-based molecular diversity of local and exotic wheat genotypes

“…They highlighted their usefulness for wheat improvement and hybridization. Furthermore, Ejaz et al (2015) used the same method to study diversity among 10 cytoplasmic male sterile CMS lines and their restorers and found similar results, and emphasized the power of molecular markers for detecting genetic variations. Similarly, the results of the present study were somewhat consistent with those reported by Filiz (2012) who screened 30 Russian wheat genotypes collected from CIMMYT (International Maize and Wheat Improvement Center) using SRAP markers.…”

Genetic diversity within local and introduced cultivars of wheat (Triticum aestivum L.) grown under Mediterranean environment as revealed by AFLP markers

“…The use of molecular markers for wheat genetic studies has recently received a great deal of attention from wheat breeders. However, the efficiency of polymorphism detection by amplified fragment length polymorphism (AFLP) in wheat is high compared with other available marker systems (Martos et al 2005;Ejaz et al 2015) since the AFLP technique combines the RFLP reliability with the power of PCR to amplify simultaneously many restriction fragments (Vos et al 1995).…”