Assessment of a six-year national survey and eradication program for Plum pox virus in Turkey

Abstract

Plum pox virus (PPV) is the most dangerous viral agent of stone fruits. PPV can be transmitted by vector aphids and may cause serious damage on fruits, leaves and flowers of Prunus species, especially on plum, apricot, and peach. Eradication of infected plants is one of the most recommended control methods of PPV in the world. The main objective of this study is to evaluate the National PPV survey and eradication program carried by the National Plant Health Authority between 2013 and 2018. During the six years of study, approximately 60.000 trees in 96.26% of Turkey were screened for PPV symptoms and 21.394 samples were collected from suspected plants from seven different fruit species. The samples were tested by ELISA (Enzyme Linked Immunosorbent Assay), and then ELISA questionable samples were verified by RT-PCR (Reverse Transcription Polymerase Chain Reaction). Based on the six years results, 2.718 samples from 161 locations were determined as PPV positive and the infection rate was calculated as 12.70%. During this study, 78.868 trees were eradicated. By the help of intensive monitoring and eradication program, PPV infection rate decreased from 35.72% to 7.47%. On the other hand, PPV infection was firstly recorded and eradicated in seven provinces (Aydın, Bolu, Denizli, Erzincan, Kırıkkale, Sivas, Samsun) which have been knownas PPV-free before. This study showed that eradication is a very effective way to suppress PPV infection and spread. In conclusion, the national survey and eradication program should be operated nationwide and concentrated in nurseries.