Assessing the effects of basal media on the in vitro propagation and nutritional status of Eucalyptus dunnii Maiden

Oberschelp, Gustavo Pedro Javier; Gonçalves, Antônio Natal

doi:10.1007/s11627-015-9740-7

Cited by 26 publications

(23 citation statements)

References 38 publications

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“…Studies comparing forest products, such as those provided by Eucalyptus, have also shown reduced environmental impact and reduced costs of delivered biomass of their products (Daystar et al 2014). Therefore, Corymbia An Acad Bras Cienc (2017) 2 GILVANO E. BRONDANI et al and Eucalyptus have been among the major trees to which researchers have devoted their studies, especially considering strategies for efficiently cloning superior genotypes, individual species or hybrids (Nourissier and Monteuuis 2008, Hung and Trueman 2011, Brondani et al 2012, Oliveira et al 2012, Baccarin et al 2015, Oberschelp and Gonçalves 2016. In recent decades, increasing research has been devoted to breeding Corymbia and Eucalyptus.…”

Section: Introductionmentioning

confidence: 99%

Mini-incubators improve the adventitious rooting performance of Corymbia and Eucalyptus microcuttings according to the environment in which they are conditioned

Brondani

Oliveira

Konzen

et al. 2018

An. Acad. Bras. Ciênc.

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We addressed a major challenge in the in vitro clonal propagation of Corymbia citriodora, Eucalyptus urophylla and E. benthamii by using an ex vitro adventitious rooting strategy in a mini-incubator. Miniincubators were placed in four environments for rooting. A shade house with no fogging system and a greenhouse with no ventilation but with a fogging environment had the best performance in terms of rooting, root growth and survival of microcuttings. Daily recording of the temperature within each miniincubator in each environment allowed the verification of negative correlations between the maximum average temperature and the survival, adventitious rooting and root growth. The ideal maximum air temperature for the efficient production of clonal plants was 28.4°C (± 5.5°C), and the minimum was 20.3°C (± 6.2°C). E. benthamii was more sensitive to higher temperatures than C. citriodora and E. urophylla. Nevertheless, placing mini-incubators in the shade house with no fogging system resulted in a stable and uniform performance among the three species, with 100.0% survival and 81.4% rooting. Histological sections of the adventitious roots revealed connection with the stem vascular cambium. Therefore, our experimental system demonstrated the potential of mini-incubators coupled with the proper environment to optimize the adventitious rooting performance of microcuttings.

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Section: Introductionmentioning

confidence: 99%

Mini-incubators improve the adventitious rooting performance of Corymbia and Eucalyptus microcuttings according to the environment in which they are conditioned

Brondani

Oliveira

Konzen

et al. 2018

An. Acad. Bras. Ciênc.

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“…Prior to bioreactor cultivation, explants were preconditioned in thin layer culture (TLC) using halfstrength MS without hormones. Oberschelp & Gonçalves (2016) found that decreasing the salt content in MS medium could increase the number of shoots and roots, so this approach was adopted. Pre-conditioning was carried out to adapt the explants in liquid medium and subsequently allowing the optimal plant response upon cultured in bioreactors.…”

Section: Resultsmentioning

confidence: 99%

Shoot multiplication and growth rates of Aquilaria malaccensis Lamk. shoot cultures in temporary immersion system (TIS)-RITA® and bubble column bioreactors

Esyanti¹,

Fadholi²,

Rizki³

et al. 2019

PAK.J.BOT.

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Agarwood is the resinous product of several tree species of family Thymelaeaceae such as Aquilaria spp. Unsustainable harvesting has threatened the existence of agarwood producing species in their natural habitat. Therefore, the present study was developed to facilitate an effective and efficient method to produce Aquilaria malaccensis seedlings by In vitro culture. Here, we optimized the aeration rate in the bubble column reactor and the immersion time on the temporary immersion system (TIS)-RITA ® bioreactor and compare the growth rate of A. malaccensis with both systems. A. malaccensis shoot cultures were propagated in Murashige and Skoog (MS) semisolid medium and then pre-conditioned in thin layer culture before bioreactor cultivation. A. malaccensis shoots in the bubble column reactor were subjected to variable aeration rates of 0.05 and 0.1 vvm, while the immersion variation within the TIS-RITA ® bioreactor was 5 and 15 min for every 4 h. The results showed that 15 min immersion for every 4 h increased the number of A. malaccensis shoot regeneration. Nonetheless, no significance difference was observed on biomass acquisition in both bubble column bioreactor and TIS-RITA ® bioreactor. Therefore, our findings indicate that immersion period was more critical than aeration rate for A. malaccensis shoot cultivation.

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“…In this regard, Richard-Forget et al [15] report that the thiol group of L-cysteine traps free radicals and quinones by inhibiting the enzyme polyphenol oxidase. The use of this amino acid to reduce in vitro oxidation has been reported by Hussain et al [16] in black pepper (Piper Nigrum),, Oberschelp and Gonçalves [17] in eucalyptus (Eucalyptus dunnii Maideny) and Akhtar and Shahzad [18] in sandalwood (Santalum album L.).…”

Section: Treatments With Antioxidant Agentsmentioning

confidence: 99%

Evaluation of different antioxidants during in vitro establishment of allspice (Pimenta dioica L. Merrill): a recalcitrant species

Vásquez-Hernández

Cruz‐Cruz

Santiago-Santiago³

et al. 2020

Preprint

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Background: Pimenta dioica L. Merrill is a tree whose fruits are used as a spice due to their culinary and therapeutic uses. Conventional propagation techniques using seeds and cuttings do not guarantee the phytosanitary quality of this crop. Therefore, the use of Plant Tissue Culture techniques are an option for in vitro establishment. The aim of this study was to evaluate the effect of different antioxidant agents (Methylene blue, L-cysteine and silver nanoparticles) added to MS (Murashige and Skoog) culture medium at different concentrations (0, 50, 100 and 200 mg L-1) during axenic establishment of buds used as explants of P. dioica. Results: The percentage of survival, oxidation and contamination was determined, as well as the content of soluble phenols, cell wall-linked phenols, antioxidant capacity and lipid peroxidation. Results showed significant differences among the different antioxidants for the evaluated variables; the highest survival occurred in the treatments with the addition of L-cysteine with percentages greater than 40 %, while the lowest survival occurred in the control treatment, 100 and 200 mg L-1 methylene blue, with 0, 3.3 and 0% survival, respectively. The highest percentage of oxidation was observed in the control treatments, 100 and 200 mg L-1 methylene blue with 96.67% oxidation, while the lowest percentages were observed in explants treated with L-cysteine, with 30% oxidation. Treatments with 100 and 200 mg L-1 AgNPs had the lowest contamination values, with 20%. Biochemical determinations showed that L-cysteine and 50 and 100 mgL-1 AgNPs resulted in an increase in the content of soluble phenols. The highest contents of cell wall-linked phenols were obtained in treatments with 200 mg L-1 methylene blue, L-cysteine, and 200 mg L-1 AgNPs. Analysis of antioxidant capacity revealed that all treatments had a reaction of scavenging / reduction mechanisms free radicals. Regarding lipid peroxidation, the highest content of malondialdehydes was observed in the control treatment and 200 mgL-1 methylene blue. Conclusion: the addition of L-cysteine to the culture medium showed a higher survival rate, decreased oxidation, greater production of phenolic compounds, increased antioxidant capacity and decreased lipid peroxidation, this amino acid being an alternative to reduce oxidation during in vitro introduction of allspice and other species that exhibit recalcitrance in vitro during establishment.

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Assessing the effects of basal media on the in vitro propagation and nutritional status of Eucalyptus dunnii Maiden

Cited by 26 publications

References 38 publications

Mini-incubators improve the adventitious rooting performance of Corymbia and Eucalyptus microcuttings according to the environment in which they are conditioned

Mini-incubators improve the adventitious rooting performance of Corymbia and Eucalyptus microcuttings according to the environment in which they are conditioned

Shoot multiplication and growth rates of Aquilaria malaccensis Lamk. shoot cultures in temporary immersion system (TIS)-RITA® and bubble column bioreactors

Evaluation of different antioxidants during in vitro establishment of allspice (Pimenta dioica L. Merrill): a recalcitrant species

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