Assessing Genetic Diversity using RAPD Molecular markers in  Coffea canephora Pierre ex. Froehner (Robusta coffee): A Step  towards Crop Improvement

Awati, Mallikarjun; Tambat, B.; D'Souza, G. F.; Venkataramanan, D.; Kumar, Manish; Anand, C. G.; Raghuramulu, Y.

doi:10.20546/ijcmas.2018.712.198

Cited by 3 publications

(1 citation statement)

References 9 publications

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“…Studies on genetic diversity of Robusta coffee based on RAPD markers have also been reported (Awati et al 2018;Achar et al 2015: Tshilenge et al 2009Diniz et al 2005;Cabral et al 2002). Still, such a study is needed on Robusta coffee grown in a particular agroclimatic condition, such as that of Lampung.…”

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confidence: 99%

Genetic diversity among 24 clones of robusta coffee in Lampung based on RAPD markers

et al. 2021

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Abstract. Ramadiana S, Hapsoro D, Evizal R, Setiawan K, Karyanto A, Yusnita. 2021. Genetic diversity among 24 clones of robusta coffee in Lampung based on RAPD markers. Biodiversitas 22: 3122-3129. This study aimed to estimate the genetic diversity among 24 clones of Robusta coffee from Lampung, Indonesia, by use of RAPD markers. The clones consisted of 18 local and 6 BP clones. These BP clones were developed from a breeding program of The Indonesian Coffee and Cocoa Research Institute. Genomic DNAs extracted from these clones were subjected to polymerase chain reaction and the amplified products were run using gel electrophoresis. Eleven random primers produced clear, reproducible, scorable bands. Fifty-four of 86 bands showed polymorphism and were used to construct a dendrogram based on UPGMA Jaccard's Similarity Coefficients. The genetic base of the population was narrow (average genetic similarity 68.4%), ranging from 26-93%. The genetic similarity of the local clones was higher than that of BP clones. The clones were clustered into five groups. Group 1 contained one clone (BP 534), while each of Group II-V contained more than one clone. The average genetic similarity of BP 534 to each clone of Group II-V was 41%. The genetic similarity of clones in Group II, III, IV, and V were 55.5%, 43.0%, 81.1%, and 80.1%, respectively. This research should be very useful for selecting parents in a breeding program to produce better clones of Robusta coffee.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity among 24 clones of robusta coffee in Lampung based on RAPD markers

et al. 2021

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Phenotypic and genotypic variability among exotic arabica coffee genotypes using morphological and molecular markers (SRAP)

Gokavi,

Gangadharappa,

Satish

et al. 2023

Ecological Genetics and Genomics

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Genetic diversity of Coffea canephora Pierre ex A. Froehner in Temanggung District, Indonesia based on molecular marker RAPD

2021

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Abstract. Authors. 2021. Genetic diversity of Coffea canephora Pierre ex A. Froehner in Temanggung District, Indonesia based on molecular marker RAPD. Biodiversitas 22: 4775-4783. Temanggung District in Central Java Province, Indonesia is one of robusta coffee production centers. The condition of coffee plantations in Temanggung shows variations in some morphological traits. Variations in coffee phenotypes are considered less profitable for farmers because they produce yields of undesirable quality in the global market. This study aimed to evaluate the genetic diversity of robusta coffee in Temanggung. The coffee plants were derived from six villages located at two levels of altitude. The morphological traits were observed from canopy width, trunk diameter, plant height, cherry volume, and bean volume, while the biochemical compositions were determined by caffeine content and brew’s pH value. The molecular assays were performed using PCR-RAPD with ten primers and species identification was based on the ITS rDNA. Our finding showed a variation in all morphological characters and biochemical compositions based on the LSD test 5%. The molecular marker RAPD revealed the genetic diversity by showing the DNA polymorphism levels of 95%, with the genetic similarity coefficient ranged from 0.35 to 0.86. The species identification also demonstrated that our robusta coffee was 97.11-99.70% similar to robusta coffee MK615737.1 from Philippines and robusta coffee DQ153593.1 from Cameroon. Thus, genetic diversity on six populations of robusta coffee was found, along with its variations on phenotypes which might lead the coffee yield quality to become uneven.

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Assessing Genetic Diversity using RAPD Molecular markers in Coffea canephora Pierre ex. Froehner (Robusta coffee): A Step towards Crop Improvement

Cited by 3 publications

References 9 publications

Genetic diversity among 24 clones of robusta coffee in Lampung based on RAPD markers

Genetic diversity among 24 clones of robusta coffee in Lampung based on RAPD markers

Phenotypic and genotypic variability among exotic arabica coffee genotypes using morphological and molecular markers (SRAP)

Genetic diversity of Coffea canephora Pierre ex A. Froehner in Temanggung District, Indonesia based on molecular marker RAPD

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