Genetic diversity among 24 clones of robusta coffee in Lampung based on RAPD markers

Ramadiana, Sri; Hapsoro, Dwi; Evizal, Rusdi; Setiawan, Kukuh; Karyanto, Agus; Yusnita, Yusnita

doi:10.13057/biodiv/d220614

Cited by 3 publications

(1 citation statement)

References 29 publications

(29 reference statements)

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“…Similarity index values were 1.13 (OPA12) and 1.00 each for (OPY20 and OPC04) while Null frequency values ranges from 0.05 to 0.34. Several authors have assessed the genetic diversity/relatedness of plant species with RAPD primers [29,30,26,22,31]. In the work of Srilekha and Ravi, [22], 100 percent polymorphism was observed in OPE-4 and OPA-9 primers similar to the finding of this research.…”

Section: Resultssupporting

confidence: 76%

Intra Specific Relationships and Biochemical Composition of Laportea Aestuans (L.) Chew, Sida Rhombifolia L. And Commelina Verginica L. in Lagos State

E. Ani,

Ofodile,

Nwakanma

et al. 2023

Iraqi Journal of Science

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Laportea aestuans L. (Chew), Commelina virginica (L.) and Sida rhombifolia (L.) are common wild plants used in treating several ailments including diarrhea, dysentery, hernia, oedema, ulcers and many more in traditional African medicine especially, Nigeria. The potentials of Random Amplified polymorphic DNA (RAPD) primers in delimiting intra-specific variation in L. aestuans, C. virginica and S. rhombifolia was assessed using three RAPD primers. Plant and soil samples were collected from 19 local government areas in Lagos State and assessed for genetic and biochemical relationships. A total of 56 bands were produced of which 44 were polymorphic. Maximum number of bands (21) was produced by OPY20 while OPC04 produced minimum number of bands (14). The maximum and minimum percentage polymorphisms were 100% (OPC04) and 50% (OPA12), The dendrogram of genetic diversity had a genetic distance range of 0.57 to 1.00, 0.58 to 1.00 and 0.52 to 1.00 and clustered at 0.57, 0.58, 0.52 implying 57%, 58%, 52% similarity and 43%, 42% and 48% variability for L. aestuans, C. virginica and S. rhombifolia respectively. Results of phytochemical analysis show that S. rhombifolia is particularly high in steroids (I134.19±0.23), flavonoids (118.65±0.18), phenolics (125.25±1.43), alkaloids (63.56±0.11) and triterpenes (72.44±0.12) but low in saponins (23.29±0.05), glycosides (6.79±0.01) and tannins (7.10±0.02).

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Section: Resultssupporting

confidence: 76%

Intra Specific Relationships and Biochemical Composition of Laportea Aestuans (L.) Chew, Sida Rhombifolia L. And Commelina Verginica L. in Lagos State

E. Ani,

Ofodile,

Nwakanma

et al. 2023

Iraqi Journal of Science

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Applications of omics technologies in Coffea

Mishra¹,

Jingade²,

Huded³

2022

Omics in Horticultural Crops

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Estimating the Genetic Diversity of Oranges Citrus spp. in South Sulawesi, Indonesia, Using RAPD Markers

Tuwo,

Kuswinanti,

Nasruddin

et al. 2023

Scientifica

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Oranges hold significant economic importance, being cultivated extensively worldwide and having a large global market. Indonesia, ranked eighth globally as a producer of oranges, is one of the countries with high genetic diversity of oranges. This diversity is distributed across various regions of Indonesia, including South Sulawesi. Despite the advancements in DNA-based molecular marker techniques for assessing genetic diversity, information on orange diversity in South Sulawesi is currently unavailable and under-researched. In this study, random amplified polymorphic DNA (RAPD) markers were utilized to analyze the genetic diversity of oranges in five production centers in South Sulawesi. Leaf samples of 13 orange varieties were collected from the five production centers: Pangkep, Sidrap, Bantaeng, North Luwu, and Selayar in South Sulawesi, Indonesia. Genomic DNA extraction from the orange leaves followed the protocol of the DNA Mini Kit Geneaid. DNA amplification was carried out using the RAPD method with 14 primers: OPE-04, OPH-04, OPH-15, OPN-14, OPN-16, OPR-08, OPR-20, OPW-06, OPW-09, OPX-07, OPX-11, OPX-17, UBC-18, and UBC-51. The RAPD primers yielded 109 amplified fragments ranging in size from 200 to 2000 base pairs (bp), and all RAPD primers showed 100% polymorphism. The genetic diversity value (He) of oranges in South Sulawesi was moderate (0.236). Cluster analysis based on a similarity coefficient of 77% divided the 175 orange genotypes into five groups. The most closely related genotypes were SB6 and SB7, exhibiting 100% similarity, followed by genotypes JS8 and JS9 and JS13 and JS17, with genetic similarities exceeding 99% for each pair. Genotypes P9 and SI5 displayed the highest genetic distance, with a similarity coefficient of 57%. The dendrogram diagram can serve as a basis for selecting desired plant traits in the improvement of plant characteristics through both conventional breeding and genetic engineering activities.

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Genetic diversity among 24 clones of robusta coffee in Lampung based on RAPD markers

Cited by 3 publications

References 29 publications

Intra Specific Relationships and Biochemical Composition of Laportea Aestuans (L.) Chew, Sida Rhombifolia L. And Commelina Verginica L. in Lagos State

Intra Specific Relationships and Biochemical Composition of Laportea Aestuans (L.) Chew, Sida Rhombifolia L. And Commelina Verginica L. in Lagos State

Applications of omics technologies in Coffea

Estimating the Genetic Diversity of Oranges Citrus spp. in South Sulawesi, Indonesia, Using RAPD Markers

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