Assay of superoxide dismutase activity in animal tissues

Nandi, Anuradha; Chatterjee, Indu B.

doi:10.1007/bf02712155

Cited by 229 publications

(133 citation statements)

References 23 publications

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“…Malondialdehyde (MDA) was estimated by thiobarbituric acid reactive substances (TBARS) test protocol from Satoh K at 532 nm by ELICO-SL-104 double beam UL-UV Spectrophotometer. 12 Superoxide dismutase (SOD) activity was estimated on the inhibition of the formation of NADH-phenazine methosulphate-nitro blue tetrazolium formazon from modified protocol of Nandi A et al 13 The colour formed at the end of the reaction and measured at 560nm by ELICO-SL-104 double beam UL-UV Spectrophotometer. Reduced glutathione (GR) level was estimated on the development of yellow colour when 5'5' dithiobis (2-nitrobenzioc acid) was added to sulphydryl compound test protocol from Butler E et al 14 This reaction was read at 420 nm by ELICO-SL-104 double beam UL-UV Spectrophotometer.…”

Section: Sample Collection and Bio Chemical Assaymentioning

confidence: 99%

Oxidative stress in cigarette smoker and smokeless tobacco user among ethnic group north-eastern population of Uttar Pradesh, India

2017

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Smokeless tobacco may also refer to tobacco dipping, snuff, snus, creamy snuff or tobacco toothpaste, tobacco gum, dissolvable tobacco, topical tobacco paste, tobacco water, herbal smokeless tobacco, etc. Smokeless tobacco use is more prevalent in countries of Asia, Africa and the Middle East than in Europe and the Americas. 5Cigarette smoke (CS) contains over 4000 chemical compounds such as nicotine, nitrogen oxides, carbon monoxide, hydrogen cyanide and free radicals, leading to generation of more toxic and carcinogenic compounds harmful to the health. 6 Nicotine is a major addictive agent in tobacco which acts as a psychoactive drug.CS causes metabolic activation leads to formation of reactive intermediates and potentially damaging metabolites that can promote cell injury and elicit toxic ABSTRACT Background: Cigarette smoking and other form of tobacco abusing habits are high prevalence in India at present which can be compare the oxidative level among them. This study aimed to measure the oxidative level among different cigarette smoke and other form of users in north-eastern, Uttar Pradesh of India. Methods: Total of 934 male and female subjects were selected in which 387 were controls (Group I), 140 were active smokers (Group II), 105 were passive smokers (group III), 182 were tobacco users (Group IV) and 120 were active smokers plus tobacco users (group V). Cigarette smoker and tobacco user prevalence <10/day, >10/day for 5 years' duration were collected. MDA, SOD, GR and CAT were measured. Results: Cigarette smoker and tobacco user prevalence is high in >10 cigarette/day for 5 years, Mean and SD value in oxidative stress in cigarette smokers and tobacco users MDA level 1314±330.1µmol/mg is increased, whereas SOD, GR and CAT level 2.229±0.248 units/ml, 0.0152±0.0071mg/ml and 0.345±0.046mg/ml respectively were reduced in active smoker plus tobacco users. Conclusions: Present study concluded that cigarette smoker and tobacco user showed their increased MDA and decreased SOD, GR and CAT which represented the significantly increased oxidative stress in north-eastern of Uttar Pradesh, India.

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Section: Sample Collection and Bio Chemical Assaymentioning

confidence: 99%

Oxidative stress in cigarette smoker and smokeless tobacco user among ethnic group north-eastern population of Uttar Pradesh, India

2017

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“…SOD was assayed utilizing the technique of Nandi, et al (1988) 10 . This method utilizes the inhibition of autooxidation of pyrogallol by superoxide dismutase enzyme.…”

Section: Assay Of Superoxide Dismutase Activity (Sod)mentioning

confidence: 99%

Antioxidant status and level of oxidants in patients of coronary heart disease

Jha¹,

Parchwani²,

Ahmad³

2013

Int J of Biomed & Adv Res

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“…Glutathione peroxidase activity (GSH-Px) was estimated in erythrocytes, as described by Paglia and Valentine 12) and as modified by Lawrence and Burk 13) . Superoxide dismutase (SOD) was estimated in serum by the pyrogallol autooxidation assay, as described by Marklund and Marklund 14) and as modified by Nandi and Chatterjee 15) . Ascorbate was estimated in serum by the dinitrophenyl hydrazine assay 16) .…”

Section: Biochemical Analysesmentioning

confidence: 99%

Effect of High Fat Diet Without Cholesterol Supplementation on Oxidative Stress and Lipid Peroxidation in New Zealand White Rabbits

Sabitha

Vasudevan

Kamath

2010

JAT

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Aim: Dietary fats may affect coronary artery disease risk by influencing factors other than serum cholesterol. The effect of diets containing coconut oil and sunflower oil without cholesterol supplementation on oxidative stress and lipid peroxidation was studied in male New Zealand White rabbits. Methods: Animals assigned to four groups (control, cholesterol-fed, coconut oil-fed and sunflower oil-fed), given an isocaloric diet and studied for 6 months. The lipid profile, reduced glutathione, glutathione peroxidase, superoxide dismutase, vitamin C and lipid peroxidation were evaluated at the beginning of the study, at the third month and at the end of the study period. Results: Serum lipid values did not show significant variation between animals fed coconut oil and sunflower oil, but total cholesterol, triglycerides and LDL-cholesterol were significantly higher and HDL-cholesterol was reduced in cholesterol-fed animals. Lipid peroxidation was higher in cholesterol-fed and sunflower oil-fed rabbits compared to controls and coconut oil-fed rabbits. Though other parameters such as reduced glutathione, glutathione peroxidase, superoxide dismutase and ascorbate did not vary between the two oil-fed rabbit groups, cholesterol-fed rabbits showed severe oxidative stress. Conclusion: We conclude that in the absence of cholesterol supplementation, coconut oil intake up to 30% of daily energy supply did not cause hypercholesterolemia or oxidative stress in rabbits.J Atheroscler Thromb, 2010; 17:213-218.

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Assay of superoxide dismutase activity in animal tissues

Cited by 229 publications

References 23 publications

Oxidative stress in cigarette smoker and smokeless tobacco user among ethnic group north-eastern population of Uttar Pradesh, India

Oxidative stress in cigarette smoker and smokeless tobacco user among ethnic group north-eastern population of Uttar Pradesh, India

Antioxidant status and level of oxidants in patients of coronary heart disease

Effect of High Fat Diet Without Cholesterol Supplementation on Oxidative Stress and Lipid Peroxidation in New Zealand White Rabbits

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