Effect of High Fat Diet Without Cholesterol Supplementation on Oxidative Stress and Lipid Peroxidation in New Zealand White Rabbits

Abstract

Aim: Dietary fats may affect coronary artery disease risk by influencing factors other than serum cholesterol. The effect of diets containing coconut oil and sunflower oil without cholesterol supplementation on oxidative stress and lipid peroxidation was studied in male New Zealand White rabbits. Methods: Animals assigned to four groups (control, cholesterol-fed, coconut oil-fed and sunflower oil-fed), given an isocaloric diet and studied for 6 months. The lipid profile, reduced glutathione, glutathione peroxidase, superoxide dismutase, vitamin C and lipid peroxidation were evaluated at the beginning of the study, at the third month and at the end of the study period. Results: Serum lipid values did not show significant variation between animals fed coconut oil and sunflower oil, but total cholesterol, triglycerides and LDL-cholesterol were significantly higher and HDL-cholesterol was reduced in cholesterol-fed animals. Lipid peroxidation was higher in cholesterol-fed and sunflower oil-fed rabbits compared to controls and coconut oil-fed rabbits. Though other parameters such as reduced glutathione, glutathione peroxidase, superoxide dismutase and ascorbate did not vary between the two oil-fed rabbit groups, cholesterol-fed rabbits showed severe oxidative stress. Conclusion: We conclude that in the absence of cholesterol supplementation, coconut oil intake up to 30% of daily energy supply did not cause hypercholesterolemia or oxidative stress in rabbits.J Atheroscler Thromb, 2010; 17:213-218.

Biochemical and Ultrastructural Cardiac Changes Induced by High-Fat Diet in Female and Male Prepubertal Rabbits

“…In our control rabbits, protection via ascorbic acid in females is better compared to males; however under HFD, the AA levels decrease in the two genders as mentioned in the study of Moor de Burgos et al [ 6 ], Sethi et al [ 63 ], and Sabitha et al [ 64 ].…”

Effect of Dietary Fats on Oxidative-Antioxidative Status of Blood in Rats

“…This is consistent with recent study showing enhancement of systemic lipid peroxidation in rabbits fed with high amount of sunflower oil [46]. In addition, high DNA adducts level as markers for DNA-damage derived from lipid peroxidation products were found in the liver of sunflower oil fed rats [47].…”

A randomized study of coconut oil versus sunflower oil on cardiovascular risk factors in patients with stable coronary heart disease

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