Aspects of Bioanalytical Method Validation for the Quantitative Determination of Trace Elements

Levine, Keith; Tudan, Christopher; Grohse, Peter; Weber, Frank; Levine, Michael A.; Kim, Yu-Seon J

doi:10.4155/bio.11.163

Cited by 13 publications

(9 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This technique allows precise quantification of the gold ion content in biological samples. 43 The uptake of citrate-GNS by B cells was confirmed, with approximately 10 μg of gold measured per 10 6 cells (calculated by dividing measured GNP mass per total cell count and multiplying by 10 6 ) ( Figure 3B). This corresponds to approximately 1% GNP-B cell association as a function of measured GNP mass per total exposed GNP mass.…”

Section: Gnps With Different Polymer Coatings and Shapesmentioning

confidence: 77%

“…To quantify the uptake of GNPs with human B cells and to compare this to the association by other APCs, we measured the gold content within B cells and MDMs after exposure to 20 μg/mL of GNPs by using inductively coupled plasma mass spectroscopy (ICP-MS). This technique allows precise quantification of the gold ion content in biological samples . The uptake of citrate-GNS by B cells was confirmed, with approximately 10 μg of gold measured per 10 6 cells (calculated by dividing measured GNP mass per total cell count and multiplying by 10 6 ) (Figure B).…”

Section: Resultsmentioning

confidence: 99%

“…The results fulfill the precision (RSD ≤ 15%) and accuracy (100% ± 15%) criteria. 43 Flow Cytometry: Cell Viability and Immune Cell Status. Cells were stained for 15 min at room temperature with a Zombie NIR Fixable Viability Kit (BioLegend, USA) and washed with FACS buffer (1% BSA, 0.5% sodium azide (Sigma-Aldrich, Switzerland) in PBS).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Polymer-Coated Gold Nanospheres Do Not Impair the Innate Immune Function of Human B Lymphocytes in Vitro

et al. 2019

View full text Add to dashboard Cite

Gold nanoparticles (GNPs) are intended for use within a variety of biomedical applications due to their physicochemical properties. Although, in general, biocompatibility of GNPs with immune cells such as macrophages and dendritic cells is well established, the impact of GNPs on B lymphocyte immune function remains to be determined. Since B lymphocytes play an important role in health and disease, the suitability of GNPs as a B cell-targeting tool is of high relevance. Thus, we provide information on the interactions of GNPs with B lymphocytes. Herein, we exposed freshly isolated human B lymphocytes to a set of well-characterized and biomedically relevant GNPs with distinct surface (polyethylene glycol (PEG), PEG/poly(vinyl alcohol) (PEG/PVA)) and shape (spheres, rods) characteristics. Polymer-coated GNPs poorly interacted with B lymphocytes, in contrast to uncoated GNPs. Importantly, none of the GNPs significantly affected cell viability, even at the highest concentration of 20 μg/mL over a 24 h suspension exposure period. Furthermore, none of the nanosphere formulations affected the expression of activation markers (CD69, CD86, MHC II) of the naive B lymphocytes, nor did they cause an increase in the secretion of pro-inflammatory cytokines (i.e. , IL-6, IL-1β). However, the absence of polymer coating on the sphere GNPs and the rod shape caused a decrease in IL-6 cytokine production by activated B lymphocytes, suggesting a functional impairment. With these findings, the present study contributes imperative knowledge toward the safe-by-design approaches being conducted to benefit the development of nanomaterials, specifically those as theranostic tools.

show abstract

Section: Gnps With Different Polymer Coatings and Shapesmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Polymer-Coated Gold Nanospheres Do Not Impair the Innate Immune Function of Human B Lymphocytes in Vitro

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Therefore, regardless of the atomic spectrometric technique, an increasingly global economy will place greater emphasis on the development and validation of rugged bioanalytical methods that are compliant with harmonized Guidance and are readily transferable to CROs in several countries. The approach to validating elemental analytical methods in biological systems is described in the article by Levine et al in this issue [15].…”

Section: Validating Icp-ms/oes Bioanalytical Methodsmentioning

confidence: 99%

The Status of Trace Elements Analysis in Biological Systems

Tudan¹,

Weber

Levine

2011

Bioanalysis

Self Cite

View full text Add to dashboard Cite

“…In order to draw sound scientific conclusions from bioanalysis research projects, it is important to have a robust validated analytical method. [6,7] While some methods may focus on the analysis of a single analyte to investigate causal relationships between the analyte and the health outcome, biological systems are often more complicated and require multifactorial analyses to elucidate the complex interrelationships of a broad panel of analytes, or “-omics” type approaches. “Metallomics” is an encompassing term for the study of metals and their function in cells that has seen increased visibility and attention in recent years.…”

Section: Introductionmentioning

confidence: 99%

Validation of a Metallomics Analysis of Placenta Tissue by Inductively-Coupled Plasma Mass Spectrometry

Harrington

Young

Fry

et al. 2015

Biol Trace Elem Res

View full text Add to dashboard Cite

Trace elements can play an important role in maternal health and fetal development, and deficiencies in some essential minerals including zinc and copper have been correlated in some individuals to the development of birth defects and adverse health outcomes later in life. The exact etiology of conditions like preeclampsia and the effects of fetal exposure to toxic metals has not been determined, making the assessment of trace element levels crucial to the elucidation of the causes of conditions like preeclampsia. Previous studies analyzing serum and placenta tissue have produced conflicting findings, suggesting the need for a robust, validated sample preparation and analysis method for the determination of trace elements in placenta. In this report, an acid digestion method and analysis by ICP-MS for a broad metallomics/mineralomics panel of trace elements is developed and validated over three experimental days for inter- and intraday precision and accuracy, linear range, matrix impact, and dilution verification. Spike recovery experiments were performed for the essential elements chromium (Cr), iron (Fe), cobalt (Co), nickel (Ni), copper (Cu), and zinc (Zn), and the toxic elements arsenic (As), cadmium (Cd), and lead (Pb) at levels equal to and in excess of native concentrations in control placenta tissue. The validated method will be essential for the development of scientific studies of maternal health and toxic metal exposure effects in childhood.

show abstract

Aspects of Bioanalytical Method Validation for the Quantitative Determination of Trace Elements

Cited by 13 publications

References 18 publications

Polymer-Coated Gold Nanospheres Do Not Impair the Innate Immune Function of Human B Lymphocytes in Vitro

Polymer-Coated Gold Nanospheres Do Not Impair the Innate Immune Function of Human B Lymphocytes in Vitro

The Status of Trace Elements Analysis in Biological Systems

Validation of a Metallomics Analysis of Placenta Tissue by Inductively-Coupled Plasma Mass Spectrometry

Contact Info

Product

Resources

About